Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts

Biochemical and Biophysical Research Communications

Volumn 427, Issue 4, 2012, Pages 785-789

  Recolin, Bénédicte ^a,b   Maiorano, Domenico ^a  

^a INSTITUTE OF HUMAN GENETICS   (France)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

ATR; Chk1; Replication protein A; Replication stress

Indexed keywords

APHIDICOLIN; ATR PROTEIN; CHECKPOINT KINASE 1; PROTEIN SERINE THREONINE KINASE; REPLICATION PROTEIN A 32; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE S PHASE; CONTROLLED STUDY; DENSITOMETRY; DNA DAMAGE; DNA REPLICATION; DNA SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; WESTERN BLOTTING; XENOPUS;

ANIMALS; CELL EXTRACTS; CELLS, CULTURED; DNA REPLICATION; MUTATION; OVUM; PHOSPHORYLATION; REPLICATION PROTEIN A; S PHASE CELL CYCLE CHECKPOINTS; SIGNAL TRANSDUCTION; XENOPUS LAEVIS;

EID: 84868192373     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.09.139     Document Type: Article

References (29)

1. Cimprich K.A., Cortez D. ATR: an essential regulator of genome integrity. Nat. Rev. Mol. Cell Biol. 2008, 9:616-627.
2. Walter J., Newport J. Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. Mol. Cell 2000, 5:617-627.
3. Byun T.S., Pacek M., Yee M.C., Walter J.C., Cimprich K.A. Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint. Genes Dev. 2005, 19:1040-1052.
4. Zou L., Elledge S.J. Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science 2003, 300:1542-1548.
5. Costanzo V., Shechter D., Lupardus P.J., Cimprich K.A., Gottesman M., Gautier J. An ATR- and Cdc7-dependent DNA damage checkpoint that inhibits initiation of DNA replication. Mol. Cell 2003, 11:203-213.
6. Van C., Yan S., Michael W.M., Waga S., Cimprich K.A. Continued primer synthesis at stalled replication forks contributes to checkpoint activation. J. Cell Biol. 2010, 189:233-246.
7. Recolin B., Van der Laan S., Maiorano D. Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts. Nucleic Acids Res. 2012, 40:3431-3442.
8. Wu X., Shell S.M., Zou Y. Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. Oncogene 2005, 24:4728-4735.
9. Shiotani B., Zou L. Single-stranded DNA orchestrates an ATM-to-ATR switch at DNA breaks. Mol. Cell 2009, 33:547-558.
10. Lambert S., Carr A.M. Checkpoint responses to replication fork barriers. Biochimie 2005, 87:591-602.
11. Adachi Y., Laemmli U.K. Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts. EMBO J. 1994, 13:4153-4164.
12. Mimura S., Masuda T., Matsui T., Takisawa H. Central role for cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts. Genes Cells 2000, 5:439-452.
13. Wold M.S. Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Annu. Rev. Biochem. 1997, 66:61-92.
14. Robison J.G., Elliott J., Dixon K., Oakley G.G. Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks. J. Biol. Chem. 2004, 279:34802-34810.
15. Carty M.P., Zernik-Kobak M., McGrath S., Dixon K. UV light-induced DNA synthesis arrest in HeLa cells is associated with changes in phosphorylation of human single-stranded DNA-binding protein. EMBO J. 1994, 13:2114-2123.
16. Liu V.F., Weaver D.T. The ionizing radiation-induced replication protein A phosphorylation response differs between ataxia telangiectasia and normal human cells. Mol. Cell. Biol. 1993, 13:7222-7231.
17. Shao R.G., Cao C.X., Zhang H., Kohn K.W., Wold M.S., Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999, 18:1397-1406.
18. Nuss J.E., Patrick S.M., Oakley G.G., Alter G.M., Robison J.G., Dixon K., Turchi J.J. DNA damage induced hyperphosphorylation of replication protein A. 1. identification of novel sites of phosphorylation in response to DNA damage. Biochemistry 2005, 44:8428-8437.
19. Vassin V.M., Wold M.S., Borowiec J.A. Replication protein A (RPA) phosphorylation prevents RPA association with replication centers. Mol. Cell. Biol. 2004, 24:1930-1943.
20. Murray A.W. Cell cycle extracts. Methods Cell Biol. 1991, 36:581-605.
21. Cuvier O., Lutzmann M., Mechali M. ORC is necessary at the interphase-to-mitosis transition to recruit cdc2 kinase and disassemble RPA foci. Curr. Biol. 2006, 16:516-523.
22. Olson E., Nievera C.J., Klimovich V., Fanning E., Wu X. RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint. J. Biol. Chem. 2006, 281:39517-39533.
23. Shechter D., Costanzo V., Gautier J. ATR and ATM regulate the timing of DNA replication origin firing. Nat. Cell Biol. 2004, 6:648-655.
24. Marheineke K., Hyrien O. Control of replication origin density and firing time in Xenopus egg extracts: role of a caffeine-sensitive ATR-dependent checkpoint. J. Biol. Chem. 2004, 279:28071-28081.
25. Michael W.M., Ott R., Fanning E., Newport J. Activation of the DNA replication checkpoint through RNA synthesis by primase. Science 2000, 289:2133-2137.
26. Francon P., Lemaitre J.M., Dreyer C., Maiorano D., Cuvier O., Mechali M. A hypophosphorylated form of RPA34 is a specific component of pre-replication centers. J. Cell Sci. 2004, 117:4909-4920.
27. Binz S.K., Lao Y., Lowry D.F., Wold M.S. The phosphorylation domain of the 32-kDa subunit of replication protein A (RPA) modulates RPA-DNA interactions. Evidence for an intersubunit interaction. J. Biol. Chem. 2003, 278:35584-35591.
28. Liu S., Opiyo S.O., Manthey K., Glanzer J.G., Ashley A.K., Amerin C., Troksa K., Shrivastav M., Nickoloff J.A., Oakley G.G. Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress. Nucleic Acids Res. 2012.
29. Lindsey-Boltz L.A., Reardon J.T., Wold M.S., Sancar A. In vitro analysis of the role of RPA and RPA phosphorylation in ATR-mediated checkpoint signaling. J. Biol. Chem. 2012.