The characterization and quantitation of glycomic changes in CHO cells during a bioreactor campaign

Biotechnology and Bioengineering

Volumn 109, Issue 12, 2012, Pages 3007-3017

  Tep, Samnang ^a   Hincapie, Marina ^b   Hancock, William S ^b  

^a BIOGEN   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

Author keywords

CHO; Glycomics; Isotopic label; MALDI TOF MS

Indexed keywords

BIOTECHNOLOGY INDUSTRY; CHO; CHO CELL; DEGLYCOSYLATION; GLYCANS; GLYCOMICS; INTRACELLULAR PROTEINS; ISOTOPIC LABELS; ISOTOPIC TAGS; LINEAR DYNAMIC RANGES; MALDI TOF MS; ORDERS OF MAGNITUDE; SIMULTANEOUS ANALYSIS;

AFFINITY CHROMATOGRAPHY; BIOREACTORS; CELLS; DIGITAL STORAGE; ISOTOPES; PROTEINS;

CYTOLOGY;

CELL PROTEIN; GLYCAN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BIOREACTOR; BIOTECHNOLOGY; CHO CELL; DEGLYCOSYLATION; GLYCOBIOLOGY; GLYCOSYLATION; ISOTOPE LABELING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; QUANTITATIVE ANALYSIS;

ANIMALS; BIOREACTORS; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOMICS; GLYCOPROTEINS; GLYCOSYLATION; ISOTOPE LABELING; ORTHO-AMINOBENZOATES; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; POLYSACCHARIDES; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAPHYLOCOCCAL PROTEIN A;

EID: 84868134349     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24590     Document Type: Article

References (39)

1. Ahn J, Bones J, Yu YQ, Rudd PM, Gilar M. 2010. Separation of 2-aminobenzamide labeled glycans using hydrophilic interaction chromatography columns packed with 1.7μm sorbent. J Chromatogr B 878(3-4):403-408.
2. Alvarez-Manilla G, Warren NL, Abney T, Atwood J, Azadi P, York WS, Pierce M, Orlando R. 2007. Tools for glycomics: Relative quantitation of glycans by isotopic permethylation using 13CH3I. Glycobiology 17(7):677-687.
3. Atwood JA III, Cheng L, Alvarez-Manilla G, Warren NL, York WS, Orlando R. 2008. Quantitation by isobaric labeling: Applications to glycomics. J Proteome Res 7(1):367-374.
4. Bowman MJ, Zaia J. 2007. Tags for the stable isotopic labeling of carbohydrates and quantitative analysis by mass spectrometry. Anal Chem 79(15):5777-5784.
5. Carlage T, Hincapie M, Zang L, Lyubarskaya Y, Madden H, Mhatre R, Hancock WS. 2009. Proteomic profiling of a high-producing Chinese hamster ovary cell culture. Anal Chem 81(17):7357-7362.
6. Ciucanu I, Kerek F. 1984. A simple and rapid method for the permethylation of carbohydrates. Carbohydr Res Vol 131(2):209-217.
7. Darling RJ, Kuchibhotla U, Glaesner W, Micanovic R, Witcher DR, Beals JM. 2002. Glycosylation of erythropoietin affects receptor binding kinetics: Role of electrostatic interactions. Biochemistry 41(49):14524-14531.
8. Ebe Y. 2006. Application of lectin microarray to crude samples: Differential glycan profiling of lec mutants. J Biochem 139(3):323-327.
9. Gu X, Wang DI. 1998. Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine. Biotechnol Bioeng 58(6):642-648.
10. Harvey DJ. 2005. Collision-induced fragmentation of negative ions from N-linked glycans derivatized with 2-aminobenzoic acid. J Mass Spectrom 40(5):642-653.
11. Hirabayashi J. 2004. Lectin-based structural glycomics: Glycoproteomics and glycan profiling. Glycoconj J 21(1-2):35-40.
12. Julka S, Regnier F. 2004. Quantification in proteomics through stable isotope coding: A review. J Proteome Res 3(3):350-363.
13. Kurihara T, Min JZ, Hirata A, Toyo'oka T, Inagaki S. 2009. Rapid analysis of N-linked oligosaccharides in glycoproteins (ovalbumin, ribonuclease B and fetuin) by reversed-phase ultra-performance liquid chromatography with fluorescence detection and electrospray ionization time-of-flight mass spectrometry. Biomed Chromatogr 23(5):516-523.
14. Li H, d'Anjou M. 2009. Pharmacological significance of glycosylation in therapeutic proteins. Curr Opin Biotechnol 20(6):678-684.
15. Misaizu T, Matsuki S, Strickland TW, Takeuchi M, Kobata A, Takasaki S. 1995. Role of antennary structure of N-linked sugar chains in renal handling of recombinant human erythropoietin. Blood 86(11):4097-4104.
16. Niwa R, Hatanaka S, Shoji-Hosaka E, Sakurada M, Kobayashi Y, Uehara A, Yokoi H, Nakamura K, Shitara K. 2004. Enhancement of the antibody-dependent cellular cytotoxicity of low-fucose IgG1 Is independent of FcgammaRIIIa functional polymorphism. Clin Cancer Res 10(18 Pt 1):6248-6255.
17. Orlando R, Lim JM, Atwood JA, 3rd, Angel PM, Fang M, Aoki K, Alvarez-Manilla G, Moremen KW, York WS, Tiemeyer M, Pierce M, Dalton S, Wells L. 2009. IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J Proteome Res 8(8):3816-3823.
18. Packer NH, Lawson MA, Jardine DR, Redmond JW. 1998. A general approach to desalting oligosaccharides released from glycoproteins. Glycoconj J 15(8):737-747.
19. Papac DI, Wong A, Jones AJ. 1996. Analysis of acidic oligosaccharides and glycopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem 68(18):3215-3223.
20. Presta LG. 2008. Molecular engineering and design of therapeutic antibodies. Curr Opin Immunol 20(4):460-470.
21. Raju TS, Stanley P. 1996. LEC14, a dominant Chinese hamster ovary glycosylation mutant expresses complex N-glycans with a new N-acetylglucosamine residue in the core region. J Biol Chem 271(13):7484-7493.
22. Raju TS, Ray MK, Stanley P. 1995. LEC18, a dominant chinese hamster ovary glycosylation mutant synthesizes N-linked carbohydrates with a novel core structure. J Biol Chem 270:30294-30302.
23. Ridlova G, Mortimer JC, Maslen SL, Dupree P, Stephens E. 2008. Oligosaccharide relative quantitation using isotope tagging and normal-phase liquid chromatography/mass spectrometry. Rapid Commun Mass Spectrom 22(17):2723-2730.
24. Shields RL. 2002. Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. J Biol Chem 277(30):26733-26740.
25. Shinkawa T, Nakamura K, Yamane N, Shoji-Hosaka E, Kanda Y, Sakurada M, Uchida K, Anazawa H, Satoh M, Yamasaki M, et al. 2002. The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity. J Biol Chem 278(5):3466-3473.
26. Spiro RG. 1973. Glycoproteins. Adv Protein Chem 27:349-467.
27. Stanley P. 1989. Chinese Hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity. Mol Cell Biol 9:377-383.
28. Takeuchi M, Inoue N, Strickland TW, Kubota M, Wada M, Shimizu R, Hoshi S, Kozutsumi H, Takasaki S, Kobata A. 1989. Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. Proc Natl Acad Sci USA 86(20):7819-7822.
29. Tateno H, Uchiyama N, Kuno A, Togayachi A, Sato T, Narimatsu H, Hirabayashi J. 2007. A novel strategy for mammalian cell surface glycome profiling using lectin microarray. Glycobiology 17(10):1138-1146.
30. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, et al. 2006. Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells. Biotechnol Bioeng 94(6):1045-1052.
31. Wuhrer M, Deelder AM. 2005. Negative-mode MALDI-TOF/TOF-MS of oligosaccharides labeled with 2-aminobenzamide. Anal Chem 77(21):6954-6959.
32. Wuhrer M, Koeleman CAM, Deelder AM. 2009. Two-Dimensional HPLC Separation with Reverse-Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools After Labeling with 2-Aminobenzamide. In: Packer NH, Karlsson NG, editors. Methods in Molecular Biology, Glycomics: Methods and Protocols. Human Press. p 1-13.
33. Xia B, Feasley CL, Sachdev GP, Smith DF, Cummings RD. 2009. Glycan reductive isotope labeling for quantitative glycomics. Anal Biochem 387(2):162-170.
34. Yamane-Ohnuki N, Kinoshita S, Inoue-Urakubo M, Kusunoki M, Iida S, Nakano R, Wakitani M, Niwa R, Sakurada M, Uchida K, et al. 2004. Establishment ofFUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity. Biotechnol Bioeng 87(5):614-622.
35. Yang M, Butler M. 2002. Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms. Biotechnol Prog 18(1):129-138.
36. Yoon SK, Choi SL, Song JY, Lee GM. 2005. Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0C. Biotechnol Bioeng 89(3):345-356.
37. Yu YQ, Ahn J, Gilar M. 2010. Trastuzumab Glycan Batch-to-Batch Profiling using a UPLC/FLR/Mass Spectrometry Platform - Application Note. Milford, MA: Waters Corporation. p 1-8.
38. Yuan J, Hashii N, Kawasaki N, Itoh S, Kawanishi T, Hayakawa T. 2005. Isotope tag method for quantitative analysis of carbohydrates by liquid chromatography-mass spectrometry. J Chromatogr A 1067(1-2):145-152.
39. Zhou Q, Shankara S, Roy A, Qiu H, Estes S, McVie-Wylie A, Culm-Merdek K, Park A, Pan C, Edmunds T. 2008. Development of a simple and rapid method for producing non-fucosylated oligomannose containing antibodies with increased effector function. Biotechnol Bioeng 99(3):652-665.