Smad7 inhibits differentiation and mineralization of mouse osteoblastic cells

Endocrine Journal

Volumn 59, Issue 8, 2012, Pages 653-662

  Yano, Masato ^a   Inoue, Yoshifumi ^b   Tobimatsu, Takako ^b   Hendy, Geoffrey N ^c   Canaff, Lucie ^c   Sugimoto, Toshitsugu ^d   Seino, Susumu ^b   Kaji, Hiroshi ^a,b  

^a KINKI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b KOBE UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c ROYAL VICTORIA HOSPITAL   (Canada)

^d SHIMANE UNIVERSITY FACULTY OF MEDICINE   (Japan)

Author keywords

Bone morphogenetic protein; Osteoblast; Parathyroid hormone; Smad; Transforming growth factor

Indexed keywords

ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN; COLLAGEN TYPE 1; CYCLINE; MESSENGER RNA; PARATHYROID HORMONE[1-34]; SMAD6 PROTEIN; SMAD7 PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME INHIBITION; HORMONE ACTION; MINERALIZATION; MOUSE; NONHUMAN; OSTEOBLAST; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; SIGNAL TRANSDUCTION;

ALKALINE PHOSPHATASE; ANIMALS; CELL DIFFERENTIATION; COLLAGEN TYPE I; MICE; OSTEOBLASTS; PARATHYROID HORMONE; RNA, SMALL INTERFERING; SMAD6 PROTEIN; SMAD7 PROTEIN;

EID: 84867904765     PISSN: 09188959     EISSN: 13484540     Source Type: Journal    
DOI: 10.1507/endocrj.EJ12-0022     Document Type: Article

References (28)

1. Massague J (1998) TGF-β signal transduction. Annu Rev Biochem 67: 753-791.
2. Bhushan A, Chen Y, Vale W (1998) Smad7 inhibits mesoderm formation and promotes neural cell fate in Xenopus embryos. Dev Biol 200: 260-268.
3. Itoh S, Landstrom M, Hermansson A, Itoh F, Heldin CH, Heldin NE, ten Dijke P (1998) Transforming growth factor β1 induces nuclear export of inhibitory Smad7. J Biol Chem 273: 29195-29201.
4. Wang EA, Rosen V, D'Alessandro JS, Bauduy M, Cordes P, Harada T, Israel DI, Hewick RM, Kerns KM, LaPan P, Luxenberg DH, McQuiad D, Moutsatsos IK, Nove J, Wozney JM (1990) Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci USA 87: 2220-2224.
5. Harris SE, Sabatini M, Harris MA, Feng JQ, Wozney J, Mundy GR (1994) Expression of bone morphogenetic protein messenger RNA in prolonged cultures of fetal rat calvarial cells. J Bone Miner Res 9: 389-394.
6. Chen D, Harris M A, Rossini G, Dunstan CR, Dallas SL, Feng JQ, Mundy GR, Harris SE (1997) Bone morphogenetic protein 2 (BMP-2) enhances BMP-3, BMP-4, and bone cell differentiation marker gene expression during the induction of mineralized bone matrix formation in cultures of fetal rat calvarial osteoblasts. Calcif Tissue Int 60: 283-290.
7. Ju W, Hoffmann A, Verschueren K, Tylzanowski P, Kaps C, Gross G, Huylebroeck D (2000) The bone morphogenetic protein 2 signaling mediator Smad1 participates predominantly in osteogenic and not in chondrogenic differentiation in mesenchymal progenitors C3H10T1/2. J Bone Miner Res 15: 1889-1899.
8. Nishimura R, Kato Y, Chen D, Harris SE, Mundy GR, Yoneda T (1998) Smad5 and DPC4 are key molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal precursor cell line C2C12. J Biol Chem 273: 1872-1879.
9. Janssens K, ten Dijke P, Janssens S, Van Hul W (2005) Transforming growth factor-β1 to the bone. Endocr Rev 26: 743-774.
10. Sowa H, Kaji H, Yamaguchi T, Sugimoto T, Chihara K (2002) Smad3 promotes alkaline phosphatase activity and mineralization of osteoblastic MC3T3-E1 cells. J Bone Miner Res 17: 1190-1199.
11. Sowa H, Kaji H, Yamaguchi T, Sugimoto T, Chihara K (2002) Activations of ERK1/2 and JNK by transforming growth factor β negatively regulate Smad3-induced alkaline phosphatase activity and mineralization in mouse osteoblastic cells. J Biol Chem 277: 36024-36031.
12. Borton AJ, Frederick JP, Datto MB, Wang XF, Weinstein RS (2001) The loss of Smad3 results in a lower rate of bone formation and osteopenia through dysregulation of osteoblast differentiation and apoptosis. J Bone Miner Res 16: 1754-1764.
13. Sowa H, Kaji H, Iu MF, Tsukamoto T, Sugimoto T, Chihara K (2003) Parathyroid hormone-Smad3 axis exerts anti-apoptotic action and augments anabolic action of transforming growth factor β in osteoblasts. J Biol Chem 278: 52240-52252.
14. Tobimatsu T, Kaji H, Sowa H, Naito J, Canaff L, Hendy, G.N., Sugimoto T, Chihara K (2006) Parathyroid hormone increases β-catenin levels through Smad3 in mouse osteoblastic cells. Endocrinology 147: 2583-2590.
15. Inoue Y, Canaff L, Hendy GN, Hisa I, Sugimoto T, Chihara K, Kaji H (2009) Role of Smad3, acting independently of transforming growth factor-β, in the early induction of Wnt-beta-catenin signaling by parathyroid hormone in mouse osteoblastic cells. J Cell Biochem 108: 285-294.
16. Fujii M, Takeda K, Imamura T, Aoki H, Sampath TK, Enomoto S, Kawabata M, Kato M, Ichijo H, Miyazono K (1999) Roles of bone morphogenetic protein type I receptors and Smad proteins in osteoblast and chondroblast differentiation. Mol Biol Cell 10: 3801-3813.
17. Choy L, Skillington J, Derynck R (2000) Roles of autocrine TGF-β receptor and Smad signaling in adipocyte differentiation. J Cell Biol 149: 667-682.
18. Valcourt U, Gouttenoire J, Moustakas A, Herbage D, Mallein-Gerin F (2002) Functions of transforming growth factor-β family type I receptors and Smad proteins in the hypertrophic maturation and osteoblastic differentiation of chondrocytes. J Biol Chem 277: 33545-33558.
19. Hiraki Y, Inoue H, Shigeno C, Sanma Y, Bentz H, Rosen DM, Asada A, Suzuki F (1991) Bone morphogenetic protein (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitro. J Bone Miner Res 6: 1373-1385.
20. Higuchi C, Myouji A, Hashimoto N, Kuriyama K, Yoshioka K, Yoshikawa H, Itoh K (2002) Continuous inhibition of MAPK signaling promotes the early osteoblastic differentiation and mineralization of the extracellular matrix. J Bone Miner Res 17: 1785-1794.
21. Canalis E, Economides AN, Gazzerro E (2003) Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr Rev 24: 218-235.
22. Zhu HJ, Iaria J, Sizeland AM (1999) Smad7 differentially regulates transforming growth factor β-mediated signaling pathways. J Biol Chem 274: 32258-32264.
23. Nakao A, Afrakhte M, Moren A, Nakayama T, Christian JL, Heuchel R, Itoh S, Kawabata M, Heldin NE, Heldin CH, ten Dijke P (1997) Identification of Smad7, a TGF-β-inducible antagonist of TGF-β signaling. Nature 389: 631-635.
24. Afrakhte M, Moren A, Jossan S, Itoh S, Smapath K, Westermark B, Heldin CH, Heldin NE, ten Dijke P (1998) Induction of inhibitory Smad6 and Smad7 mRNA by TGF-β family members. Biochem Biophys Res Commun 249: 505-511.
25. Nagarajan RP, Zhang J, Li W, Chen Y (1999) Regulation of Smad7 promoter by direct association with Smad3 and Smad4. J Biol Chem 274: 33412-33418.
26. von Gersdorff G, Susztak K, Rezvani F, Bitzer M, Liang D, Bottinger EP (2000) Smad3 and Smad4 mediate transcriptional activation of the human Smad7 promoter by transforming growth factor β. J Biol Chem 275: 11320-11326.
27. Denissova NG, Pouponnot C, Long J, He D, Liu F (2000) Transforming growth factor β-inducible independent binding of SMAD to the Smad7 promoter. Proc Natl Acad Sci USA 97: 6397-6402.
28. Stopa M, Anhuf D, Terstegen L, Gatsios P, Gressner AM, Dooley S (2000) Participation of Smad2, Smad3, and Smad4 in transforming growth factor β (TGF-β)-induced activation of Smad7: the TGF-β response element of the promoter requires functional Smad binding element and E-box sequences for transcriptional regulation. J Biol Chem 275: 29308-29317.