Viral latency drives 'memory inflation': A unifying hypothesis linking two hallmarks of cytomegalovirus infection

Medical Microbiology and Immunology

Volumn 201, Issue 4, 2012, Pages 551-566

  Seckert, Christof K ^a   Grießl, Marion ^a   Büttner, Julia K ^a   Scheller, Sabine ^a   Simon, Christian O ^a   Kropp, Kai A ^a   Renzaho, Angélique ^a   Kühnapfel, Birgit ^a   Grzimek, Natascha K A ^a   Reddehase, Matthias J ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Antigen presentation; CD8 T cells; Cytomegalovirus; Endothelial cells; Hematopoietic cell transplantation (HCT); Immune surveillance; Latency associated transcripts; Latent infection; Liver sinusoidal endothelial cells; Memory inflation; Memory T cells; Viral latency; Virus reactivation

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; EPITOPE; INTERLEUKIN 7 RECEPTOR; KILLER CELL IMMUNOGLOBULIN LIKE RECEPTOR; L SELECTIN; MESSENGER RNA;

ANTIGEN PRESENTATION; CD8+ T LYMPHOCYTE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; CYTOPATHOGENIC EFFECT; DISEASE MODEL; EFFECTOR CELL; ENDOTHELIUM CELL; GENE EXPRESSION; GENE LOCUS; GENE SILENCING; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; HYPOTHESIS; IMMEDIATE EARLY GENE; IMMUNOCOMPROMISED PATIENT; IMMUNOLOGICAL MEMORY; IMMUNOSURVEILLANCE; LIVER SINUSOID; MEMORY CELL; NONHUMAN; ORGAN TRANSPLANTATION; PRIORITY JOURNAL; REVIEW; VIRUS GENE; VIRUS GENOME; VIRUS LATENCY; VIRUS REACTIVATION;

ANIMALS; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DISEASE MODELS, ANIMAL; HUMANS; IMMUNOLOGIC MEMORY; MICE; VIRUS LATENCY;

EID: 84867893617     PISSN: 03008584     EISSN: 14321831     Source Type: Journal    
DOI: 10.1007/s00430-012-0273-y     Document Type: Review

References (92)

1. Seo S, Boeckh M (2013) Clinical cytomegalovirus research: hematopoietic cell transplantation. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol II, chap 16. Caister Academic Press, Norfolk, UK (in press)
2. Avery RK (2013) Clinical cytomegalovirus research: thoracic organ transplantation. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol II, chap 13. Caister Academic Press, Norfolk, UK (in press)
3. Emery VC, Milne RS, Griffiths PD (2013) Clinical cytomegalovirus research: liver and kidney transplantation. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol II, chap 14. Caister Academic Press, Norfolk, UK (in press)
4. Reeves M, Sinclair J (2008) Aspects of human cytomegalovirus latency and reactivation. In: Shenk TE, Stinski MF (eds) Human cytomegalovirus. Current Topics in Microbiology and Immunology, vol 325. Springer, Berlin, pp 297-314
5. Reeves M, Sinclair J (2013) Epigenetic regulation of human cytomegalovirus gene expression: impact on latency and reactivation. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 19. Caister Academic Press, Norfolk, UK (in press)
6. Slobedman B, Avdic S, Abendroth A (2013) Transcription associated with human cytomegalovirus latency. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 20. Caister Academic Press, Norfolk, UK (in press)
7. Smith MS, Streblow DN, Caposio P, Nelson JA (2013) Humanized mouse models of cytomegalovirus pathogenesis and latency. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 23. Caister Academic Press, Norfolk, UK (in press)
8. Reddehase MJ, Podlech J, Grzimek NK (2002) Mouse models of cytomegalovirus: overview. J Clin Virol 25:S23-S36
9. Hummel M, Abecassis MM (2002) A model for reactivation of CMV from latency. J Clin Virol 25:S123-S136
10. Reddehase MJ, Simon CO, Seckert CK, Lemmermann N, Grzimek NK (2008) Murine model of cytomegalovirus latency and reactivation. In: Shenk TE, Stinski MF (eds) Human cytomegalovirus. Current Topics in Microbiology and Immunology, vol 325. Springer, Berlin, pp 315-331
11. Seckert CK, Grießl M, Büttner JK, Freitag K, Lemmermann NA, Hummel MA, Liu XF, Abecassis MI, Angulo A, Messerle M, Cook CH, Reddehase MJ (2013) Immune surveillance of cytomegalovirus latency and reactivation in murine models: link to "memory inflation". In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 22. Caister Academic Press, Norfolk, UK (in press)
12. Emery VC (1998) Relative importance of cytomegalovirus load as a risk factor for cytomegalovirus disease in the immunocompromised host. In: Scholz M, Rabenau HF, Doerr HW, Cinatl J Jr (eds) Monographs in virology 21:CMV-related immunopathology. Karger, Basel, pp 288-301
13. Davison AJ, Holton M, Dolan A, Dargan DJ, Gatherer D, Hayward GS (2013) Comparative genomics of primate cytomegaloviruses. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 1. Caister Academic Press, Norfolk, UK (in press)
14. Adler B, Sinzger C (2013) Cytomegalovirus inter-strain variance in cell-type tropism. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 17. Caister Academic Press, Norfolk, UK (in press)
15. Mercer JA, Wiley CA, Spector DH (1988) Pathogenesis of murine cytomegalovirus infection: identification of infected cells in the spleen during acute and latent infection. J Virol 62:987-997
16. Pomeroy C, Hilleren PJ, Jordan MC (1991) Latent murine cytomegalovirus DNA in splenic stromal cells of mice. J Virol 65:3330-3334
17. Koffron AJ, Hummel M, Patterson BK, Yan S, Kaufmann DB, Fryer JP, Stuart FP, Abecassis MI (1998) Cellular localization of latent murine cytomegalovirus. J Virol 72:95-103
18. Balthesen M, Messerle M, Reddehase MJ (1993) Lungs are a major organ site of cytomegalovirus latency and recurrence. J Virol 67:5360-5366
19. Reddehase MJ, Balthesen M, Rapp M, Jonjić S, Pavić I, Koszinowski UH (1994) The conditions of primary infection define the load of latent viral genome in organs and the risk of recurrent cytomegalovirus disease. J Exp Med 179:185-193
20. Kurz SK, Rapp M, Steffens HP, Grzimek NKA, Schmalz S, Reddehase MJ (1999) Focal transcriptional activity of murine cytomegalovirus during latency in the lungs. J Virol 73:482-494
21. Seckert CK, Renzaho A, Reddehase MJ, Grzimek NK (2008) Hematopoietic stem cell transplantation with latently infected donors does not transmit virus to immunocompromised recipients in the murine model of cytomegalovirus infection. Med Microbiol Immunol 197:251-259
22. Daley-Bauer LP, Mocarski ES (2013) Myeloid cell recruitment and function in cytomegalovirus immunity and pathogenesis. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 21. Caister Academic Press, Norfolk, UK (in press)
23. Marquardt A, Halle S, Seckert CK, Lemmermann NA, Veres TZ, Braun A, Maus UA, Förster R, Reddehase MJ, Messerle M, Busche A (2011) Single cell detection of latent cytomegalovirus reactivation in host tissue. J Gen Virol 92:1279-1291
24. Seckert CK, Renzaho A, Tervo HM, Krause C, Deegen P, Kühnapfel B, Reddehase MJ, Grzimek NKA (2009) Liver sinusoidal endothelial cells are a site of murine cytomegalovirus latency and reactivation. J Virol 83:8869-8884
25. Roizman B, Sears AE (1987) An inquiry into the mechanisms of herpes simplex virus latency. Annu Rev Microbiol 41:543-571
26. Sacher T, Podlech J, Mohr CA, Jordan S, Ruzsics Z, Reddehase MJ, Koszinowski UH (2008) The major virus-producing cell type during murine cytomegalovirus infection, the hepatocyte, is not the source of virus dissemination in the host. Cell Host Microbe 3:263-272
27. Sacher T, Andrassy J, Kalnins A, Dölken L, Jordan S, Podlech J, Ruzsics Z, Jauch KW, Reddehase MJ, Koszinowski UH (2011) Shedding light on the elusive role of endothelial cells in cytomegalovirus dissemination. PLoS Pathog 7:e1002366
28. Kern M, Popov A, Scholz K, Schumak B, Djandji D, Limmer A, Eggle D, Sacher T, Zawatzky R, Holtappels R, Reddehase MJ, Hartmann G, Debey-Pascher S, Diehl L, Kalinke U, Koszinowski U, Schultze J, Knolle PA (2010) Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity. Gastroenterology 138:336-346
29. Stinski MF, Petrik DT (2008) Functional roles of the human cytomegalovirus essential IE86 protein. In: Shenk TE, Stinski MF (eds) Human cytomegalovirus. Current Topics in Microbiology and Immunology, vol 325. Springer, Berlin, pp 133-152
30. Meier JL, Stinski MF (2013) Major immediate-early enhancers and its gene products. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 10. Caister Academic Press, Norfolk, UK (in press)
31. Mocarski ES Jr, Hahn G, Lofgren White K, Xu J, Slobedman B, Hertel L, Aguirre SA, Noda S (2006) Myeloid cell recruitment and function in pathogenesis and latency. In: Reddehase MJ (ed) Cytomegaloviruses. Caister Academic Press, Norfolk (UK), pp 465-481
32. White KL, Slobedman B, Mocarski ES (2000) Human cytomegalovirus latency-associated protein pORF94 is dispensable for productive and latent infection. J Virol 74:9333-9337
33. Kurz S, Steffens HP, Mayer A, Harris JR, Reddehase MJ (1997) Latency versus persistence or intermittent recurrences: evidence for a latent state of murine cytomegalovirus in the lungs. J Virol 71:2980-2987
34. Meyers JD, Flournoy N, Thomas ED (1982) Nonbacterial pneumonia after allogeneic marrow transplantation: a review of ten years' experience. Rev Infect Dis 4:1119-1132
35. Zaia JA (1993) Prevention and treatment of cytomegalovirus pneumonia in transplant recipients. Clin Infect Dis 17:S392-S399
36. Riddell SR (1995) Pathogenesis of cytomegalovirus pneumonia in immunocompromised hosts. Semin Respir Infect 10:199-208
37. Reddehase MJ, Weiland F, Münch K, Jonjic S, Lüske A, Koszinowski UH (1985) Interstitial murine cytomegalovirus pneumonia after irradiation: characterization of cells that limit viral replication during established infection of the lungs. J Virol 55:264-273
38. Holtappels R, Podlech J, Geginat G, Steffens HP, Thomas D, Reddehase MJ (1998) Control of murine cytomegalovirus in the lungs: relative but not absolute immunodominance of the immediate- early 1 nonapeptide during the antiviral cytolytic T-lymphocyte response in pulmonary infiltrates. J Virol 72:7201-7212
39. Podlech J, Holtappels R, Pahl-Seibert MF, Steffens HP, Reddehase MJ (2000) Murine model of interstitial cytomegalovirus pneumonia in syngeneic bone marrow transplantation: persistence of protective pulmonary CD8-T-cell infiltrates after clearance of acute infection. J Virol 74:7496-7507
40. Cook CH, Zhang Y, Sedmak DD, Martin LC, Jewell S, Ferguson RM (2006) Pulmonary cytomegalovirus reactivation causes pathology in immunocompetent mice. Crit Care Med 34:842-849
41. Grzimek NKA, Dreis D, Schmalz S, Reddehase MJ (2001) Random, asynchronous, and asymmetric transcriptional activity of enhancer-flanking major immediate-early genes ie1/3 and ie2 during murine cytomegalovirus latency in the lungs. J Virol 75:2692-2705
42. Simon CO, Seckert CK, Dreis D, Reddehase MJ, Grzimek NK (2005) Role for tumor necrosis factor alpha in murine cytomegalovirus transcriptional reactivation in latently infected lungs. J Virol 79:326-340
43. Simon CO, Holtappels R, Tervo HM, Böhm V, Däubner T, Oehrlein-Karpi SA, Kühnapfel B, Renzaho A, Strand D, Podlech J, Reddehase MJ, Grzimek NK (2006) CD8 T cells control cytomegalovirus latency by epitope-specific sensing of transcriptional reactivation. J Virol 80:10436-10456
44. Simon CO, Kühnapfel B, Reddehase MJ, Grzimek NKA (2007) Murine cytomegalovirus major immediate-early enhancer region operating as a genetic switch in bidirectional gene pair transcription. J Virol 81:7805-7810
45. Simon CO, Seckert CK, Grzimek NKA, Reddehase MJ (2006) Murine model of cytomegalovirus latency and reactivation: the silencing/desilencing and immune sening hypothesis. In: Reddehase MJ (ed) Cytomegaloviruses. Caister Academic Press, Norfolk (UK), pp 483-500
46. Boshart M, Weber F, Jahn G, Dorsch-Häsler K, Fleckenstein B, Schaffner W (1985) A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41:521-530
47. Dorsch-Häsler K, Keil GM, Weber F, Jasin M, Schaffner W, Koszinowski UH (1985) A long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mRNA in murine cytomegalovirus. Proc Natl Acad Sci USA 82:8325-8329
48. Stinski MF, Isomura H (2008) Role of the cytomegalovirus major immediate early enhancer in acute infection and reactivation from latency. Med Microbiol Immunol 197:223-231
49. Keil GM, Ebeling-Keil A, Koszinowski UH (1987) Immediateearly genes of murine cytomegalovirus: location, transcripts, and translation products. J Virol 61:526-533
50. Keil GM, Ebeling-Keil A, Koszinowski UH (1987) Sequence and structural organization of murine cytomegalovirus immediateearly gene 1. J Virol 61:1901-1908
51. Messerle M, Keil GM, Koszinowski UH (1991) Structure and expression of murine cytomegalovirus immediate-early gene 2. J Virol 65:1638-1643
52. Messerle M, Bühler B, Keil GM, Koszinowski UH (1992) Structural organization, expression, and functional characterization of the murine cytomegalovirus immediate-early gene 3. J Virol 66:27-36
53. Adachi N, Lieber MR (2002) Bidirectional gene organization: a common architectural feature of the human genome. Cell 109:807-809
54. Trinklein ND, Aldred SF, Hartman SJ, Schroeder DI, Otillar RP, Myers RM (2004) An abundance of bidirectional promoters in the human genome. Genome Res 14:62-66
55. Li YY, Yu H, Guo ZM, Guo TQ, Tu K, Li YX (2006) Systematic analysis of head-to-head gene organization: evolutionary conservation and potential biological relevance. PLoS Comput Biol 2:e74
56. Redwood AJ, Shellam GR, Smith LM (2013) Molecular evolution of murine cytomegalovirus genomes. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 2. Caister Academic Press, Norfolk, UK (in press)
57. Busche A, Angulo A, Kay-Jackson P, Ghazal P, Messerle M (2008) Phenotypes of major immediate-early gene mutants of mouse cytomegalovirus. Med Microbiol Immunol 197:233-240
58. Angulo A, Ghazal P, Messerle M (2000) The major immediateearly gene ie3 of mouse cytomegalovirus is essential for viral growth. J Virol 74:11129-11136
59. Gribaudo G, Riera L, Lembo D, De Andrea M, Gariglio M, Rudge TL, Johnson LF, Landolfo S (2000) Murine cytomegalovirus stimulates cellular thymidylate synthase gene expression in quiescent cells and requires the enzyme for replication. J Virol 74:4979-4987
60. Lembo D, Gribaudo G, Hofer A, Riera L, Cornaglia M, Mondo A, Angeretti A, Gariglio M, Thelander L, Landolfo S (2000) Expression of an altered ribonucleotide reductase activity associated with the replication of murine cytomegalovirus in quiescent cells. J Virol 74:11557-11565
61. Wilhelmi V, Simon CO, Podlech J, Böhm V, Däubner T, Emde S, Strand D, Renzaho A, Lemmermann NAW, Seckert CK, Reddehase MJ, Grzimek NKA (2008) Transactivation of cellular genes involved in nucleotide metabolism by the regulatory IE1 protein of murine cytomegalovirus is not critical for viral replicative fitness in quiescent cells and host tissues. J Virol 82:9900-9916
62. Maul GG (2008) Initiation of cytomegalovirus infection at ND10. In: Shenk TE, Stinski MF (eds) Human cytomegalovirus. Current Topics in Microbiology and Immunology, vol 325. Springer, Berlin, pp 117-132
63. Ghazal P, Visser AE, Gustems M, Garcia R, Borst EM, Sullivan K, Messerle M, Angulo A (2005) Elimination of ie1 significantly attenuates murine cytomegalovirus virulence but does not alter replicative capacity in cell culture. J Virol 79:7182-7194
64. Rodríguez-Martín S, Kropp K, Wilhelmi V, Juranic Lisnic V, Yuan Hsieh W, Blanc M, Livingston A, Busche A, Tekotte H, Messerle M, Auer M, Jonjic S, Angulo A, Reddehase MJ, Ghazal P (2012) Ablation of the regulatory IE1 protein of murine cytomegalovirus alters in vivo pro-inflammatory TNF-alpha production during acute infection. PLoS Pathog 8:e1002901
65. Cardin RD, Abenes GB, Stoddart CA, Mocarski ES (1995) Murine cytomegalovirus IE2, an activator of gene expression, is dispensable for growth and latency in mice. Virology 209:236-241
66. Chatellard P, Pankiewicz R, Meier E, Durrer L, Sauvage C, Imhof MO (2007) The IE2 promoter/enhancer region from mouse CMV provides high levels of therapeutic protein expression in mammalian cells. Biotechnol Bioeng 96:106-117
67. Kropp KA, Simon CO, Fink A, Renzaho A, Kühnapfel B, Podlech J, Reddehase MJ, Grzimek NKA (2009) Synergism between the components of the bipartite major immediate-early transcriptional enhancer of murine cytomegalovirus does not accelerate virus replication in cell culture and host tissues. J Gen Virol 90:2395-2401
68. Angulo A, Messerle M, Koszinowski UH, Ghazal P (1998) Enhancer requirement for murine cytomegalovirus growth and genetic complementation by the human cytomegalovirus enhancer. J Virol 72:8502-8509
69. Ghazal P, Messerle M, Osborn K, Angulo A (2003) An essential role of the enhancer for murine cytomegalovirus in vivo growth and pathogenesis. J Virol 77:3217-3322
70. Podlech J, Pintea R, Kropp KA, Fink A, Lemmermann NAW, Erlach KC, Isern E, Angulo A, Ghazal P, Reddehase MJ (2010) Enhancerless cytomegalovirus is capable of establishing a lowlevel maintenance infection in severe immunodeficient host tissues but fails in exponential growth. J Virol 84:6254-6261
71. Wang D, Bodovitz S (2010) Single cell analysis: the new frontier in "Omics". Trends Biotechnol 28:281-290
72. Toriello NM, Douglas ES, Thaitrong N, Hsiao SC, Francis MB, Bertozzi CR, Mathies RA (2008) Integrated microfluidic bioprocessor for single-cell gene expression analysis. Proc Natl Acad Sci USA 105:20173-20178
73. Lemmermann NA, Podlech J, Seckert CK, Kropp KA, Grzimek NK, Reddehase MJ, Holtappels R (2010) CD8 T-cell immunotherapy of cytomegalovirus disease in the murine model. In: Kabelitz D, Kaufmann SHE (eds) Methods in microbiology: immunology of infection. Academic Press, London, pp 369-420
74. Steffens HP, Kurz S, Holtappels R, Reddehase MJ (1998) Preemptive CD8 T-cell immunotherapy of acute cytomegalovirus infection prevents lethal disease, limits the burden of latent viral genomes, and reduces the risk of virus recurrence. J Virol 72:1797-1804
75. Böhm V, Seckert CK, Simon CO, Thomas D, Renzaho A, Gendig D, Holtappels R, Reddehase MJ (2009) Immune evasion proteins enhance cytomegalovirus latency in the lungs. J Virol 83:10293-10298
76. Thomas M, Reuter N, Stamminger T (2013) Multifaceted regulation of human cytomegalovirus gene expression. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol I, chap 11. Caister Academic Press, Norfolk, UK, (in press)
77. Hummel M, Zhang Z, Yan S, DePlaen I, Golia P, Varghese T, Thomas G, Abecassis MI (2001) Allogeneic transplantation induces expression of cytomegalovirus immediate-early gene in vivo: a model for reactivation from latency. J Virol 75:4814-4822
78. Cook CH, Zhang Y, McGuinness BJ, Lahm MC, Sedmak DD, Ferguson RM (2002) Intra-abdominal bacterial infection reactivates latent pulmonary cytomegalovirus in immunocompetent mice. J Infect Dis 185:1395-1400
79. Cook CH, Trgovcich J, Zimmerman PD, Zhang Y, Sedmak DD (2006) Lipopolysaccharide, tumor necrosis factor alpha, or interleukin-1beta triggers reactivation of latent cytomegalovirus in immunocompetent mice. J Virol 80:9151-9158
80. Kurz SK, Reddehase MJ (1999) Patchwork pattern of transcriptional reactivation in the lungs indicates sequential checkpoints in the transition from murine cytomegalovirus latency to recurrence. J Virol 73:8612-8622
81. Reddehase MJ, Rothbard JB, Koszinowski UH (1989) A pentapeptide as minimal antigenic determinant for MHC class I-restricted T lymphocytes. Nature 337:651-653
82. Reddehase MJ (2002) Antigens and immunoevasins: opponents in cytomegalovirus immune surveillance. Nat Rev Immunol 2:831-844
83. Reddehase MJ, Koszinowski UH (1984) Significance of herpesvirus immediate early gene expression in cellular immunity to cytomegalovirus infection. Nature 312:369-371
84. Holtappels R, Pahl-Seibert MF, Thomas D, Reddehase MJ (2000) Enrichment of immediate-early 1 (m123/pp 89) peptidespecific CD8 T cells in a pulmonary CD62L(lo) memory-effector cell pool during latent murine cytomegalovirus infection of the lungs. J Virol 74:11495-11503
85. Holtappels R, Thomas D, Podlech J, Reddehase MJ (2002) Two antigenic peptides from genes m123 and m164 of murine cytomegalovirus quantitatively dominate CD8 T-cell memory in the H-2d haplotype. J Virol 76:151-160
86. Munks MW, Cho KS, Pinto AK, Sierro S, Klenerman P, Hill AB (2006) Four distinct patterns of memory CD8 T cell responses to chronic murine cytomegalovirus infection. J Immunol 177: 450-458
87. Lemmermann NA, Kropp KA, Seckert CK, Grzimek NK, Reddehase MJ (2011) Reverse genetics modification of cytomegalovirus antigenicity and immunogenicity by CD8 T-cell epitope deletion and insertion. J Biomed Biotechnol 2011:812742
88. Torti N, Walton SM, Murphy KM, Oxenius A (2011) Batf3 transcription factor-dependent DC subsets in murine CMV infection: differential impact on T-cell priming and memory inflation. Eur J Immunol 41:2612-2618
89. Torti N, Walton SM, Brocker T, Rülicke T, Oxenius A (2011) Non-hematopoietic cells in lymph nodes drive memory CD8 T cell inflation during murine cytomegalovirus infection. PLoS Pathog 7:e1002313
90. Seckert CK, Schader SI, Ebert S, Thomas D, Freitag K, Renzaho A, Podlech J, Reddehase MJ, Holtappels R (2011) Antigen-presenting cells of haematopoietic origin prime cytomegalovirusspecific CD8 T-cells but are not sufficient for driving memory inflation during viral latency. J Gen Virol 92:1994-2005
91. Karrer U, Wagner M, Sierro S, Oxenius A, Hengel H, Dumrese T, Freigang S, Koszinowski UH, Phillips RE, Klenerman P (2004) Expansion of protective CD8+ T-cell responses driven by recombinant cytomegaloviruses. J Virol 78:2255-2264
92. Jarvis MA, Hansen SG, Nelson JA, Picker LJ, Früh K (2013) Vaccine vectors using the unique biology and immunology of cytomegalovirus. In: Reddehase MJ (ed) Cytomegaloviruses: from molecular pathogenesis to intervention, vol II, chap 21. Caister Academic Press, Norfolk, UK (in press)