The IE2 promoter/enhancer region from mouse CMV provides high levels of therapeutic protein expression in mammalian cells

Biotechnology and Bioengineering

Volumn 96, Issue 1, 2007, Pages 106-117

  Chatellard, Philippe ^a   Pankiewicz, Renata ^a   Meier, Evelyne ^a   Durrer, Laurence ^a   Sauvage, Christophe ^a   Imhof, Markus O ^a  

^a Serono International SA   (Switzerland)

Author keywords

Enhancer; Fed batch culture; Mouse CMV; Therapeutic protein expression

Indexed keywords

CELLS; CLONING; DISEASES; DRUG PRODUCTS; GENES; MOLECULAR STRUCTURE; PROTEINS;

ENHANCER; FED BATCH CULTURE; MOUSE CYTOMEGALOVIRUS (CMV); THERAPEUTIC PROTEIN EXPRESSION;

LIVING SYSTEMS STUDIES;

INTERLEUKIN 18 BINDING PROTEIN; PROTEIN;

ANIMAL CELL; ARTICLE; AUTOIMMUNE DISEASE; BIOTECHNOLOGY; CONTROLLED STUDY; CYTOMEGALOVIRUS; DRUG DESIGN; DRUG INDICATION; DRUG MANUFACTURE; DRUG RESEARCH; ENHANCER REGION; FED BATCH CULTURE; GENE ACTIVITY; GENE EXPRESSION SYSTEM; IMMEDIATE EARLY GENE; MAMMAL CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN EXPRESSION; THERAPEUTIC PROTEIN EXPRESSION;

ANIMALS; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; GENETIC VECTORS; HUMANS; IMMEDIATE-EARLY PROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANS-ACTIVATORS;

CYTOMEGALOVIRUS; MAMMALIA;

EID: 33845925860     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21172     Document Type: Article

References (26)

1. Adachi N, Lieber MR. 2002. Bidirectional gene organization: A common architectural feature of the human genome. Cell 109:807-809.
2. Angulo A, Messerle M, Koszinowski UH, Ghazal P. 1998. Enhancer requirement for murine cytomegalovirus growth and genetic complementation by the human cytomegalovirus enhancer. J Virol 72:8502-8509.
3. Boshart M, Weber F, Jahn G, Dorsch-Häsler K, Fleckenstein B, Schaffner W. 1985. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41:521-530.
4. Butler M. 2005. Animal cell cultures: Recent achievements and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotech 68:283-291.
5. Cardin RD, Abens GB, Stoddart CA, Mocarski ES. 1995. Murine cytomegalovirus IE2, an activator of gene expression, is dispensable for growth and latency in mice. Virology 209:236-241.
6. Dorsch-Häsler K, Keil GM, Weber F, Jasin M, Schaffner W, Koszinowski UH. 1985. A long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mRNA in murine cytomegalovirus. Proc Natl Acad Sci USA 82:8325-8329.
7. Eszterhas SK, Bouhassira EE, Martin DIK, Fiering S. 2002. Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position. Mol Cell Biol 22(2):469-479.
8. Fujii H, Shinya E, Shimada T. 1992. The GC box in the bidirectional promoter is essential for expression of the human dihydrofolate reductase and mismatch repair protein 1 genes. FEBS Lett 314:33-36.
9. Fussenegger M, Bailey JE, Hauser H, Mueller PP. 1999. Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends Biotech 17:35-42.
10. Garrick D, Fiering S, Martin DIK, Whitelaw E. 1998. Repeat-induced gene silencing in mammals. Nat Genet 18:56-59.
11. Grzimek NKA, Dreis D, Schmalz S, Reddehase MJ. 2001. Random, asynchronous, and asymmetric transcriptional activity of enhancer-flanking major immediate-early genes ie1/3 and ie2 during murine cytomegalovirus latency in the lungs. J Virol 75:2692-2705.
12. Hartenbach S, Fussenegger M. 2005. Autoregulated, bidirectional and multicistronic gas-inducible mammalian as well as lentiviral expression vectors. J Biotech 120:83-98.
13. Kaufman RJ, Wasley LC, Spiliotes AJ, Gossels SD, Latt SA, Larsen GR, Kay RM. 1985. Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells. Mol Cell Biol 5:1750-1759.
14. Kim DW, Uetsuki T, Kaziro Y, Yamaguchi N, Sugano S. 1990. Use of human elongation factor 1α promoter as a versatile and efficient expression system. Gene 91:217-223.
15. Kim S-Y, Lee J-H, Shin H-S, Kang H-J, Kim Y-S. 2002. The human elongation factor 1 alpha (EF-1α) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter. J Biotech 93:183-187.
16. Z, Jayasankar S, Gray D. 2004. Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco. Transgenic Res 13:143-154.
17. Makrides SC. 1999. Components for vectors for gene transfer and expression in mammalian cells. Prot Expr Purif 17:183-202.
18. McBurney MW, Mai T, Yang X, Jardine K. 2002. Evidence for repeat-induced gene silencing in cultured mammalian cells: Inactivation of tandem repeats of transfected genes. Exp Cell Res 274:1-8.
19. Messerle M, Keil GM, Koszinowski UH. 1991. Structure and expression of murine cytomegalovirus immediate-early gene 2. J Virol 65:1638-1643.
20. Messerle M, Bühler B, Keil GM, Koszinowski UH. 1992. Structural organization, expression, and functional characterization of the murine cytomegalovirus immediate-early gene 3. J Virol 66:27-36.
21. Sanford GR, Burns WH. 1996. Rat cytomegalovirus has a unique immediate early gene enhancer. Virology 222:310-317.
22. Stennett L, Riker A, Kroll T, Chamberlin J, Miki T, Nickoloff B, Le Poole I. 2004. Expression of gp100 and CDK2 in melanoma cells is not coregulated by a shared promoter region. Pigment Cell Res 17:525-532.
23. Strathdee C, McLeod M, Hall J. 1999. Efficient control of tetracycline-responsive gene expression from an autoregulated bi-directional expression vector. Genes Dev 229:21-29.
24. Trinklein ND, Aldred SF, Hartman SJ, Schroeder DI, Otillar RP, Myers RM. 2004. An abundance of bidirectional promoters in the human genome. Genome Res 14:62-66.
25. Xia W, Bringmann P, McClary J, Jones PP, Manzana W, Zhu Y, Wang S, Liu Y, Harvey S, Madlansacay MR, McLean K, Rosser MP, MacRobbie J, Olsen CL, Cobb RR. 2006. High levels of protein expression using different mammalian CMV promoters in several cell lines. Prot Expr Purif 45:115-124.
26. Xu Z-L, Mizuguchi H, Ishii-Watabe A, Uchida E, Mayumi T, Hayakawa T. 2001. Optimization of transcriptional regulatory elements for constructing plasmid vectors. Gene 272:149-156.