Reconstitution of eukaryotic translation initiation factor 3 by co-expression of the subunits in a human cell-derived in vitro protein synthesis system

Protein Expression and Purification

Volumn 87, Issue 1, 2013, Pages 5-10

  Masutani, Mamiko ^a,b   Machida, Kodai ^a   Kobayashi, Tominari ^a   Yokoyama, Shigeyuki ^b,c   Imataka, Hiroaki ^a,b  

^a UNIVERSITY OF HYOGO   (Japan)

^b RIKEN   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cell free; Co expression; Eukaryotic translation initiation factor; In vitro protein expression; Protein complex; Reconstitution

Indexed keywords

INITIATION FACTOR 3;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CELL FREE SYSTEM; GENE DELETION; GENETIC TRANSCRIPTION; GENETICS; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; PROTEIN SUBUNIT; PROTEIN SYNTHESIS;

CELL-FREE SYSTEM; CHROMATOGRAPHY, AFFINITY; EUKARYOTIC INITIATION FACTOR-3; HELA CELLS; HUMANS; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

EUKARYOTA;

EID: 84867793660     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.10.001     Document Type: Article

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