N-terminally truncated GADD34 proteins are convenient translation enhancers in a human cell-derived in vitro protein synthesis system

Biotechnology Letters

Volumn 32, Issue 7, 2010, Pages 897-902

  Mikami, Satoshi ^a   Kobayashi, Tominari ^b   Machida, Kodai ^b   Masutani, Mamiko ^a,b   Yokoyama, Shigeyuki ^a   Imataka, Hiroaki ^a,b  

^a RIKEN   (Japan)

^b UNIVERSITY OF HYOGO   (Japan)

Author keywords

Cell free system; GADD34; In vitro translation; Mammalian cells; Protein synthesis

Indexed keywords

CELL FREE PROTEIN SYNTHESIS; CELL-FREE SYSTEM; E. COLI; HUMAN CELLS; IN-VITRO; MAMMALIAN CELLS; N-TERMINALS; PROTEIN SYNTHESIS; RECOMBINANT PROTEIN;

AMINO ACIDS; CELLS; ESCHERICHIA COLI; MAMMALS; ORGANIC ACIDS;

PROTEINS;

CELL CYCLE PROTEIN; CELL EXTRACT; DIFFERENTIATION ANTIGEN; PPP1R15A PROTEIN, HUMAN; PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN SYNTHESIS;

ANTIGENS, DIFFERENTIATION; CELL CYCLE PROTEINS; CELL EXTRACTS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; PROTEIN BIOSYNTHESIS; PROTEINS; RECOMBINANT PROTEINS; SEQUENCE DELETION;

ESCHERICHIA COLI; MAMMALIA;

EID: 77953869587     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0251-7     Document Type: Article

References (7)

1. Brush MH, Shenolikar S (2008) Control of cellular GADD34 levels by the 26S proteasome. Mol Cell Biol 28: 6989-7000.
2. Brush MH, Weiser DC, Shenolikar S (2003) Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatase 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2. Mol Cell Biol 23: 1292-1303.
3. Carroll K, Elroy-Stein O, Moss B, Jagus R (1993) Recombinant vaccinia virus K3L gene product prevents activation of double-stranded RNA-dependent, initiation factor 2 alpha-specific protein kinase. J Biol Chem 268: 12837-12842.
4. Mikami S, Kobayashi T, Yokoyama S, Imataka H (2006a) A hybridoma-based in vitro translation system that efficiently synthesizes glycoproteins. J Biotechnol 127: 65-78.
5. Mikami S, Masutani M, Sonenberg N, Yokoyama S, Imataka H (2006b) An efficient mammalian cell-free translation system supplemented with translation factors. Protein Expr Purif 46: 348-357.
6. Mikami S, Kobayashi T, Masutani M, Yokoyama S, Imataka H (2008) A human cell-derived in vitro coupled transcription/translation system optimized for production of recombinant proteins. Protein Expr Purif 62: 190-198.
7. Novoa I, Zeng H, Harding HP, Ron D (2001) Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha. J Cell Biol 153: 1011-1022.