Genetic features and molecular epidemiology of Enterococcus faecium isolated in two university hospitals in Brazil

Diagnostic Microbiology and Infectious Disease

Volumn 74, Issue 3, 2012, Pages 267-271

  da Silva, Leila Priscilla Pinheiro ^a   Pitondo Silva, André ^a   Martinez, Roberto ^a   da Costa Darini, Ana Lúcia ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

CC17; E. faecium; MLST; PFGE; Tn1546; Virulence factors; VREs

Indexed keywords

DAPTOMYCIN; GENOMIC DNA; VANCOMYCIN;

ACM GENE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; BRAZIL; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; ESP GENE; GENE AMPLIFICATION; GENE DELETION; GENE INSERTION; GENOTYPE; HOUSEKEEPING GENE; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR EPIDEMIOLOGY; MOLECULAR GENETICS; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; STRAIN IDENTIFICATION; TN1546 GENE; UNIVERSITY HOSPITAL; VANA GENE; VANCOMYCIN RESISTANT ENTEROCOCCUS; VANH GENE; VANS GENE;

ADHESINS, BACTERIAL; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BRAZIL; CARBON-OXYGEN LIGASES; DAPTOMYCIN; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; ENTEROCOCCUS FAECIUM; GRAM-POSITIVE BACTERIAL INFECTIONS; HOSPITALS, UNIVERSITY; HUMANS; MICROBIAL SENSITIVITY TESTS; MOLECULAR EPIDEMIOLOGY; MULTILOCUS SEQUENCE TYPING; VANCOMYCIN; VANCOMYCIN RESISTANCE; VIRULENCE FACTORS;

EID: 84867642645     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.07.012     Document Type: Article

References (41)

1. Billsstrom H., Lund B., Sullivan A., Nord C.E. Virulence and antimicrobial resistance in clinical Enterococcus faecium. Int J Antimicrob Agents 2008, 32:38-49.
2. Bolano A., Stinchi S., Preziosi R., Bistoni F., Allegrucci M., Baldelli F., et al. Rapid methods to extract DNA and RNA from Cryptococcus neoformans. FEMS Yeast Res 2001, 1:221-224.
3. Camargo I.L.B.C., Del Peloso P.F., Da Costa C.F., Goldman G.H., Darini A.L.C. Identification of an unusual VanA element in glycopeptide-resistant Enterococcus faecium in Brazil following international transfer of a bone marrow transplant patient. Can J Microbiol 2004, 50:767-770.
4. Camargo I.L.B.C., Gilmore M.S., Darini A.L.C. Multilocus sequence typing and analysis of putative virulence factors in vancomycin-resistant and vancomycin-sensitive Enterococcus faecium isolates from Brazil. Clin Microbiol Infect 2006, 12:1123-1130.
5. Clinical and Laboratory Standards Institute (CLSI) Zone diameter and MIC interpretive standards for Enterococcus spp. v. 29, n. 3; approved standard, M2 M7 2009, CLSI, Wayne, PA.
6. D'azevedo P.A., Kacman S.B., Schmalfuss T., Silva A., Rodrigues L.F. Primeiro caso de Enterococcus resistente a vancomicina isolado em Porto Alegre, RS. J Bras Pat 2000, 36:258.
7. Dalla Costa L.M., Reynolds P.E., Souza H.A.P.H.M., Souza D.C., Palepou M.F.I., Woodford N. Characterization of a divergent van D-type resistance element from the first glycopeptide-resistant strain of Enterococcus faecium isolated in Brazil. Antimicrob Agents Chemother 2000, 44:3444-3446.
8. Darini A.L.C., Palepou M.F., Woodford N. Effects of the movement of insertion sequences on the structure of VanA glycopeptide resistance elements in Enterococcus faecium. Antimicrob Agents Chemother 2000, 44:1362-1364.
9. Dutka-Malen S., Evers S., Courvalin P. Detection of glycopeptides resistance genotype and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995, 33:24-27.
10. Fallico L., Boldrin C., Grossato A., Franchin E., De Canale E., Tommasini T., et al. Molecular epidemiology of Enterococcus faecium isolates from an Italian hospital. Infect 2011, 39:127-133.
11. Freitas A.R., Novais C., Ruiz-Garbajosa P., Coque T.M., Peixe L. Dispersion of multidrug-resistant Enterococcus faecium isolates belonging to major clonal complexes in different Portuguese settings. Appl Environ Microbiol 2009, 75:4904-4908.
12. Efm colonization-virulence gene in megaplasmids of the Enterococcus faecium CC17 polyclonal subcluster. Antimicrob Agents Chemother 2010, 45:2660-2665.
13. Handwerger S., Skoble J., Discotto L.F., Pucci M.J. Heterogeneity of the vanA gene cluster in clinical isolates of enterococci from the northeastern United States. Antimicrob Agents Chemother 1995, 39:362-368.
14. Homan W.L., Tribe D., Poznanski S., Li M., Hogg G., Spalburg E., et al. Multilocus sequence typing scheme for Enterococcus faecium. J Clin Microbiol 2002, 40:1963-1971.
15. Hsieh Y.C., Lee W.S., Ou T.Y., Hsueh P.R. Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan. Eur J Microbiol Infect Dis 2010, 29:25-30.
16. Jensen L.B., Ahrens P., Dons L., Jones R.N., Hammerum A.M., Aarestrup F.M. Molecular analysis of Tn1546 in Enterococcus faecium isolated from animals and humans. J Clin Microbiol 1998, 36:437-442.
17. Kaulfmann M.E. Pulse-field gel electrophoresis. Molecular bacteriology: protocols and clinical applications 1998, 33-50. Humana Press, New Jersey. N. Woodford, A.P. Johnson (Eds.).
18. Kerbauy G., Perugini M.R., Yamauchi L.M., Yamada-Ogatta S.F. Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil. Braz J Med Biol Res 2011, 44(3):253-257.
19. Khan M.A., Northwood J.B., Loor R.G.J., Tholen A.T., Riera E., Falcón M., et al. High prevalence of ST-78 infection associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay. Clin Microbiol Infect 2010, 16:624-627.
20. Ko K.S., Baek J.Y., Lee J.Y., Oh W.S., Peck K.R., Lee N., et al. Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Korea. J Clin Microbiol 2005, 43:2303-2306.
21. Libisch B., Lepsanovic Z., Top J., Muzslay M., Konkoly-Thege M., Gacs M. Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates from Hungary and Serbia. Scand J Infect Dis 2008, 40:778-784.
22. López M., Sáenz Y., Álvarez-Martínez M.J., Marco F., Robredo B., Rojo-Bezares B., et al. Tn1546 structures and multilocus sequence typing of vanA-containing enterococci of animal, human and food origin. J Antimicrob Chemother 2010, 65:1570-1575.
23. Novais C., Freitas A.R., Sousa J.C., Baquero F., Coque T.M., Peixe L.V. Diversity of Tn1546 and its role in the dissemination of vancomycin-resistant Enterococci in Portugal. Antimicrob Agents Chemother 2008, 52:1001-1008.
24. Palazzo I.C.V., Camargo I.L.B.C., Zanella R.C., Darini A.L.C. Evaluation of clonality in enterococci isolated in Brazil carrying Tn1546-like elements associated with vanA plasmids. FEMS Microbiol Lett 2006, 258:29-36.
25. Palazzo I.C.V., Pitondo-Silva A., Levy C.E., Darini A.L.C. Changes in vancomycin-resistant Enterococcus faecium causing outbreaks in Brazil. J Hosp Infect 2011, 1-5.
26. Palepou M.F., Adebivi A.M., Tremlett C.H., Jensen L.B., Woodford N. Molecular analysis of diverse elements mediating VanA glycopeptide resistance in enterococci. J Antimicrob Chemother 1998, 42:605-612.
27. Panesso D., Reyes J., Rincón S., Díaz L., Galloway-Peña J., Zurita J., et al. Molecular epidemiology of vancomycin-resistant Enterococcus faecium: a prospective, multicenter study in South American hospitals. J Clin Microbiol 2010, 48:1562-1569.
28. Pitondo-Silva A., Minarini L.A.R., Camargo I.L.B.C., Darini A.L.C. Clonal relationships determined by multilocus sequence typing among enteropathogenic Escherichia coli isolated in Brazil. Can J Microbiol 2009, 55:672-679.
29. Qu T.T., Chen Y.G., Yu Y.S., Lv H.X., Dong X.Q., Xiao Z., et al. Molecular characterization of vancomycin-resistant enterococci in Hangzhou, China. J Antimicrob Chemother 2007, 1403-1405.
30. Top J., Willens R., Bonten M. Emergence of CC17 Enterococcus faecium: from commensal to hospital-adapted pathogen. FEMS Immunol Med Microbiol 2008, 52:297-308.
31. Van Wamel W.J., Hendrickx A.P., Bonten M.J., Top J., Posthuma G., Willems R.J. Growth condition-dependent Esp expression by Enterococcus faecium affects initial adherence and biofilm formation. Infect Immun 2007, 75:924-931.
32. Watanabe S., Kobayashi N., Quiñones D., Nagashima S., Uehara N., Watanabe N. Genetic diversity of Enterococci harboring the high-level gentamicin resistance gene aac(6')-le-aph-(2″)-la or aph(2″)-le in a Japanese hospital. Microb Drug Resist 2009, 15:185-194.
33. Werner G., Coque T.M., Hammerum A.M., Hope R., Hryniewicz W., Johnson A., et al. Emergence and spread of vancomycin resistance among enterococci in Europe. Euro Surveill 2008, 13:1-11.
34. Werner G., Fleige C., Geringer U., Van Schaik W., Klare I., Witte W. IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium. BMC Infect Dis 2011, 11:1-8.
35. Willems R.J., Homan W., Top J., van Santen-Verheuvel M., Tribe D., Manzioros X., et al. Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals. Lancet 2001, 357:853-855.
36. Willems R.J., Top J., Van Santen M., Robinson D.A., Coque T.M., Baquero F. Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis 2005, 11:821-828.
37. Willems R.J.L., Top J., Van Den Braak N., Van Belkum A., Mevius D.J., Hendriks G., et al. Molecular diversity and evolutionary relationships of Tn1546-like elements in Enterococci from humans and animals. Antimicrob Agents Chemother 1999, 43:483-491.
38. Woodford N., Adebiyi A.M.A., Palepou M.F.I., Cookson B.D. Diversity of VanA glycopeptide resistance elements in Enterococci from humans and nonhuman sources. Antimicrob Agents Chemother 1998, 42:502-508.
39. Woodford N., Morrison D., Johnson A.P., Briant V., George R.C., Cookson B. Application of DNA probes for rRNA and vanA genes to investigation of a nosocomial cluster of vancomycin-resistant enterococci. J Clin Microbiol 1993, 31:653-658.
40. Woodford N., Stigter L. Molecular investigation of glycopeptide resistance in Gram positive bacteria. Molecular bacteriology: protocols and clinical applications 1998, 579-615. Humana Press, Totowa, NJ. N. Woodford, A.P. Johnson (Eds.).
41. Zanella R.C., Valderato F., Lovgren M., Tyrrel G.J., Bokermann S., Almeida S.C., et al. First confirmed case of a vancomycin-resistant Enterococcus faecium with VanA phenotype from Brazil: isolation from a meningitis case in São Paulo. Microb Drug Resist 1999, 5:159-161.