Molecular epidemiology of Enterococcus faecium isolates from an Italian hospital

Infection

Volumn 39, Issue 2, 2011, Pages 127-133

  Fallico, L ^a   Boldrin, C ^a   Grossato, A ^b   Franchin, E ^a,b   De Canale, E ^a   Tommasini, T ^a   Parisi, S G ^a,b   Manganelli, R ^a,b   Palu G ^a,b  

^a UNIVERSITY HOSPITAL OF PADOVA   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Enterococcus faecium; Esp; Hospital infection; MLST; PFGE; Vancomycin; VRE

Indexed keywords

BACTERIAL PROTEIN; GLYCOPEPTIDE; PROTEIN ESP4; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIAL TRANSMISSION; BACTERIUM ISOLATE; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; GENETIC CODE; HOSPITAL INFECTION; ITALY; MOLECULAR EPIDEMIOLOGY; MOLECULAR PHYLOGENY; MULTILOCUS SEQUENCE TYPING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN VARIANT; PULSED FIELD GEL ELECTROPHORESIS; VANCOMYCIN RESISTANT ENTEROCOCCUS;

ANTI-BACTERIAL AGENTS; BACTERIAL TYPING TECHNIQUES; CLUSTER ANALYSIS; CROSS INFECTION; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECIUM; GENES, BACTERIAL; GENOTYPE; GRAM-POSITIVE BACTERIAL INFECTIONS; HOSPITALS; HUMANS; ITALY; MICROBIAL SENSITIVITY TESTS; MOLECULAR EPIDEMIOLOGY; MULTILOCUS SEQUENCE TYPING; VANCOMYCIN;

EID: 79955619786     PISSN: 03008126     EISSN: None     Source Type: Journal    
DOI: 10.1007/s15010-011-0086-8     Document Type: Article

References (28)

1. Uttley AH, Collins CH, Naidoo J, et al. Vancomycin-resistant enterococci. Lancet. 1988;1:57-8. (Pubitemid 18017235)
2. Werner G, Coque TM, Hammerum AM, et al. Emergence and spread of vancomycin resistance among enterococci in Europe. Euro Surveill 2008;13. pii: 19046
3. Klare I, Konstabel C, Mueller-Bertling S, et al. Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals. Eur J Clin Microbiol Infect Dis. 2005;24:815-25. (Pubitemid 43042788)
4. Henwood CJ, Livermore DM, Johnson AP, et al. Susceptibility of gram-positive cocci from 25 UK hospitals to antimicrobial agents including linezolid. The Linezolid Study Group. J Antimicrob Chemother. 2000;46:931-40. (Pubitemid 32000604)
5. Lambiase A, Del Pezzo M, Piazza O, et al. Typing of vancomycin-resistant Enterococcus faecium strains in a cohort of patients in an Italian intensive care unit. Infection. 2007;35:428-33.
6. Theilacker C, Jonas D, Huebner J, et al. Outcomes of invasive infection due to vancomycin-resistant Enterococcus faecium during a recent outbreak. Infection. 2009;37:540-3.
7. Werner G, Klare I, Fleige C, et al. Increasing rates of vancomycin resistance among Enterococcus faecium isolated from German hospitals between 2004 and 2006 are due to wide clonal dissemination of vancomycin-resistant enterococci and horizontal spread of vanA clusters. Int J Med Microbiol. 2008;298:515-27.
8. Willems RJ, Top J, van Santen M, et al. Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis. 2005;11:821-8. (Pubitemid 40720841)
9. Shankar N, Lockatell CV, Baghdayan AS, et al. Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infect Immun. 2001; 69:4366-72. (Pubitemid 32574434)
10. Shankar V, Baghdayan AS, Huycke MM, et al. Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein. Infect Immun. 1999;67:193-200. (Pubitemid 29021648)
11. Tendolkar PM, Baghdayan AS, Shankar N, et al. The N-terminal domain of enterococcal surface protein, Esp, is sufficient for Esp-mediated biofilm enhancement in Enterococcus faecalis. J Bacteriol. 2005;187:6213-22. (Pubitemid 41263411)
12. Shankar N, Baghdayan AS, Gilmore MS. Modulation of virulence within a pathogenicity island in vancomycin-resistant Enterococcus faecalis. Nature. 2002;417:746-50. (Pubitemid 34640628)
13. Leavis H, Top J, Shankar N, et al. A novel putative enterococcal pathogenicity island linked to the esp virulence gene of Enterococcus faecium and associated with epidemicity. J Bacteriol. 2004;186:672-82. (Pubitemid 38129644)
14. Valdezate S, Labayru C, Navarro A, et al. Large clonal outbreak of multidrug-resistant CC17 ST17 Enterococcus faecium containing Tn5382 in a Spanish hospital. J Antimicrob Chemother. 2009;63:17-20.
15. Bonora MG, Ligozzi M, De Fatima M, et al. Vancomycin-resistant Enterococcus faecium isolates causing hospital outbreaks in northern Italy belong to the multilocus sequence typing C1 lineage. Microb Drug Resist. 2004;10:114-23. (Pubitemid 38879011)
16. Bonora MG, Olioso D, Lo Cascio G, et al. Phylogenetic analysis of vancomycin-resistant Enterococcus faecium genotypes associated with outbreaks or sporadic infections in Italy. Microb Drug Resist. 2007;13:171-7. (Pubitemid 47622335)
17. Stampone L, Del Grosso M, Boccia D, et al. Clonal spread of a vancomycin-resistant Enterococcus faecium strain among bloodstream-infecting isolates in Italy. J Clin Microbiol. 2005;43:1575-80.
18. Zhu X, Zheng B, Wang S, et al. Molecular characterisation of outbreak-related strains of vancomycin-resistant Enterococcus faecium from an intensive care unit in Beijing, China. J Hosp Infect. 2009;72:147-54.
19. Hsieh YC, Lee WS, Ou TY, et al. Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan. Eur J Clin Microbiol Infect Dis 2010;29:25-30.
20. Ko KS, Baek JY, Lee JY, et al. Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Korea. J Clin Microbiol. 2005;43:2303-6. (Pubitemid 40656244)
21. Standards, NCCLS. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A5. Wayne, PA, 2001.
22. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol. 1995;33:24-7. (Pubitemid 24382391)
23. Leavis HL, Willems RJ, Top J, et al. Epidemic and nonepidemic multidrug-resistant Enterococcus faecium. Emerg Infect Dis. 2003;9:1108-15. (Pubitemid 37071553)
24. Homan WL, Tribe D, Poznanski S, et al. Multilocus sequence typing scheme for Enterococcus faecium. J Clin Microbiol. 2002;40:1963-71. (Pubitemid 34596369)
25. Murchan S, Kaufmann ME, Deplano A, et al. Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol. 2003;41:1574-85. (Pubitemid 36428107)
26. Tenover FC, Arbeit RD, Goering RV, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33:2233-9.
27. Leavis HL, Bonten MJ, Willems RJ. Identification of high-risk enterococcal clonal complexes: global dispersion and antibiotic resistance. Curr Opin Microbiol. 2006;9:454-60. (Pubitemid 44397629)
28. Top J, Schouls LM, Bonten MJ, et al. Multiple-locus variable-number tandem repeat analysis, a novel typing scheme to study the genetic relatedness and epidemiology of Enterococcus faecium isolates. J Clin Microbiol. 2004;42:4503-11. (Pubitemid 39362363)