Persistent Mycoplasma genitalium infection of human endocervical epithelial cells elicits chronic inflammatory cytokine secretion

Infection and Immunity

Volumn 80, Issue 11, 2012, Pages 3842-3849

  McGowin, Chris L ^a   Annan, Rochelle S ^a   Quayle, Alison J ^a   Greene, Sheila J ^a   Ma, Liang ^a,c   Mancuso, Miriam M ^a   Adegboye, David ^b   Martin, David H ^a  

^a LOUISIANA STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b SOUTHERN UNIVERSITY AT NEW ORLEANS   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 8; MACROPHAGE INFLAMMATORY PROTEIN 1BETA; MONOCYTE CHEMOTACTIC PROTEIN 1; TOLL LIKE RECEPTOR;

ARTICLE; CELL DIVISION; CELL MIGRATION; CHRONIC INFLAMMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOTOXICITY; EPITHELIUM CELL; FEMALE; HOST CELL; HUMAN; HUMAN CELL; LEUKOCYTE; MYCOPLASMA GENITALIUM; MYCOPLASMOSIS; PHAGOCYTE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; UTERINE CERVIX EPITHELIUM; UTERINE ENDOCERVIX;

ANALYSIS OF VARIANCE; CELLS, CULTURED; CERVIX UTERI; CYTOKINES; EPITHELIAL CELLS; FEMALE; HUMANS; MYCOPLASMA GENITALIUM; MYCOPLASMA INFECTIONS; PELVIC INFLAMMATORY DISEASE; REAL-TIME POLYMERASE CHAIN REACTION; UTERINE CERVICITIS;

EID: 84867591984     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.00819-12     Document Type: Article

References (32)

1. Barrila J, et al. 2010. Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions. Nat. Rev. Microbiol. 8:791-801.
2. Baseman JB, Lange M, Criscimagna NL, Giron JA, Thomas CA. 1995. Interplay between mycoplasmas and host target cells. Microb. Pathog.19:105-116.
3. Blaylock MW, Musatovova O, Baseman JG, Baseman JB. 2004. Determination of infectious load of Mycoplasma genitalium in clinical samples of human vaginal cells. J. Clin. Microbiol. 42:746 -752.
4. Bradshaw CS, Chen MY, Fairley CK. 2008. Persistence of Mycoplasma genitalium following azithromycin therapy. PLoS One 3:e3618. doi:10.1371/journal.pone.0003618.
5. Carlsen KH, Jensen JS. 2010. Mycoplasma genitalium PCR: does freezing of specimens affect sensitivity? J. Clin. Microbiol. 48:3624 -3627.
6. Cohen CR, et al. 2007. Mycoplasma genitalium infection and persistence in a cohort of female sex workers in Nairobi, Kenya. Sex. Transm. Dis. 34:274 -279.
7. Dallo SF, Baseman JB. 2000. Intracellular DNA replication and longterm survival of pathogenic mycoplasmas. Microb. Pathog. 29:301-309.
8. Ficarra M, et al. 2008. A distinct cellular profile is seen in the human endocervix during Chlamydia trachomatis infection. Am. J. Reprod. Immunol.60:415-425.
9. Herbst-Kralovetz MM, et al. 2008. Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia. Am. J. Reprod. Immunol.59:212-224.
10. Hjorth SV, et al. 2006. Sequence-based typing of Mycoplasma genitalium reveals sexual transmission. J. Clin. Microbiol. 44:2078 -2083.
11. Isberg RR. 1991. Discrimination between intracellular uptake and surface adhesion of bacterial pathogens. Science 252:934 -938.
12. Ito S, et al. 2011. Selection of Mycoplasma genitalium strains harbouring macrolide resistance-associated 23S rRNA mutations by treatment with a single 1 g dose of azithromycin. Sex. Transm. Infect. 87:412-414.
13. Iverson-Cabral SL, Astete SG, Cohen CR, Rocha EP, Totten PA. 2006.Intrastrain heterogeneity of the mgpB gene in Mycoplasma genitalium is extensive in vitro and in vivo and suggests that variation is generated via recombination with repetitive chromosomal sequences. Infect. Immun.74:3715-3726.
14. Iverson-Cabral SL, Astete SG, Cohen CR, Totten PA. 2007. mgpB and mgpC sequence diversity in Mycoplasma genitalium is generated by segmental reciprocal recombination with repetitive chromosomal sequences.Mol. Microbiol. 66:55-73.
15. Jensen JS. 2004. Mycoplasma genitalium: the aetiological agent of urethritis and other sexually transmitted diseases. J. Eur. Acad. Dermatol. Venereol.18:1-11.
16. Jensen JS, Bjornelius E, Dohn B, Lidbrink P. 2004. Use of TaqMan 5=nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J. Clin. Microbiol. 42:683-692.
17. Jensen JS, Blom J, Lind K. 1994. Intracellular location of Mycoplasma genitalium in cultured Vero cells as demonstrated by electron microscopy. Int. J. Exp. Pathol. 75:91-98.
18. Jensen JS, Bradshaw CS, Tabrizi SN, Fairley CK, Hamasuna R. 2008. Azithromycin treatment failure in Mycoplasma genitalium-positive patients with nongonococcal urethritis is associated with induced macrolide resistance. Clin. Infect. Dis. 47:1546 -1553.
19. Jensen JS, Hansen HT, Lind K. 1996. Isolation of Mycoplasma genitalium strains from the male urethra. J. Clin. Microbiol. 34:286 -291.
20. Lusk MJ, et al. 2011. Mycoplasma genitalium is associated with cervicitis and HIV infection in an urban Australian STI clinic population. Sex. Transm. Infect. 87:107-109.
21. Ma L, et al. 2010. Genetic variation in the complete MgPa operon and its repetitive chromosomal elements in clinical strains of Mycoplasma genitalium. PLoS One 5:e15660. doi:10.1371/journal.pone.0015660.
22. Ma L, et al. 2007. Mycoplasma genitalium: an efficient strategy to generate genetic variation from a minimal genome. Mol. Microbiol. 66:220 -236.
23. Manhart LE, Broad JM, Golden MR. 2011. Mycoplasma genitalium: should we treat and how?. Clin. Infect. Dis. 53(Suppl 3):S129 -S142.
24. Manhart LE, et al. 2008. High Mycoplasma genitalium organism burden is associated with shedding of HIV-1 DNA from the cervix. J. Infect. Dis.197:733-736.
25. McGowin CL, Anderson-Smits C. 2011. Mycoplasma genitalium: an emerging cause of sexually transmitted disease in women. PLoS Pathog. 7:e1001324. doi:10.1371/journal.ppat.1001324.
26. McGowin CL, Popov VL, Pyles RB. 2009. Intracellular Mycoplasma genitalium infection of human vaginal and cervical epithelial cells elicits distinct patterns of inflammatory cytokine secretion and provides a possiblesurvival niche against macrophage-mediated killing. BMC Microbiol. 9:139. doi:10.1186/1471-2180-9-139.
27. McGowin CL, Spagnuolo RA, Pyles RB. 2010. Mycoplasma genitalium rapidly disseminates to the upper reproductive tracts and knees of female mice following vaginal inoculation. Infect. Immun. 78:726 -736.
28. Mitchell C, et al. 2011. Cervicovaginal shedding of HIV type 1 is related to genital tract inflammation independent of changes in vaginal microbiota. AIDS Res. Hum. Retroviruses 27:35-39.
29. Napierala Mavedzenge S, Weiss HA. 2009. Association of Mycoplasma genitalium and HIV infection: a systematic review and meta-analysis. AIDS 23:611-620.
30. Taylor-Robinson D, Jensen JS. 2011. Mycoplasma genitalium: from Chrysalis to multicolored butterfly. Clin. Microbiol. Rev. 24:498 -514.
31. Ueno PM, et al. 2008. Interaction of Mycoplasma genitalium with host cells: evidence for nuclear localization. Microbiology 154:3033-3041.
32. Walker J, et al. 2011. The difference in determinants of Chlamydia trachomatis and Mycoplasma genitalium in a sample of young Australian women. BMC Infect. Dis. 11:35. doi:10.1186/1471-2334-11-35.