Fluoxetine prevents development of an early stress-related molecular signature in the rat infralimbic medial prefrontal cortex. Implications for depression?

BMC Neuroscience

Volumn 13, Issue 1, 2012, Pages

  Barreto, Rafael A ^a,b,c   Walker, Frederick R ^a,b,c   Dunkley, Peter R ^a,b,c   Day, Trevor A ^a,b,c,d   Smith, Doug W ^a,b,c  

^a UNIVERSITY OF NEWCASTLE   (Australia)

^b UNIVERSITY OF NEWCASTLE   (Australia)

^c JOHN HUNTER HOSPITAL   (Australia)

^d DEAKIN UNIVERSITY   (Australia)

Author keywords

Gene expression; Microrarray; Neurotrophin; Restraint stress; Signalling pathways

Indexed keywords

BRAIN DERIVED NEUROTROPHIC FACTOR; FLUOXETINE; GLYCOGEN SYNTHASE KINASE 3BETA; MITOGEN ACTIVATED PROTEIN KINASE; NEUROTROPHIN RECEPTOR; NEUROTROPHIN RECEPTOR TYROSINE KINASE 2; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BRAIN REGION; CHRONIC STRESS; CONTROLLED STUDY; DEPRESSION; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; IMMOBILIZATION STRESS; INFRALIMBIC MEDIAL PREFRONTAL CORTEX; MALE; MENTAL STRESS; MICROARRAY ANALYSIS; NONHUMAN; PREFRONTAL CORTEX; QUANTITATIVE ANALYSIS; RAT; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

ANIMALS; ANTIDEPRESSIVE AGENTS, SECOND-GENERATION; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE TYPE 2; DISEASE MODELS, ANIMAL; FLUOXETINE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; LIMBIC SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PREFRONTAL CORTEX; PROTO-ONCOGENE PROTEINS B-RAF; RATS; RATS, SPRAGUE-DAWLEY; RECEPTOR, TRKC; RNA, MESSENGER; SIGNAL TRANSDUCTION; STRESS, PSYCHOLOGICAL; TOR SERINE-THREONINE KINASES;

EID: 84867504799     PISSN: None     EISSN: 14712202     Source Type: Journal    
DOI: 10.1186/1471-2202-13-125     Document Type: Article

References (83)

1. Hammen C. Stress and depression. Annu Rev Clin Psychol 2005, 1:293-319. 10.1146/annurev.clinpsy.1.102803.143938, 17716090.
2. Mazure C. Life stressors as risk factors in depression. Clin Psychol Sci Prac 1998, 5:291-313.
3. Ulrich-Lai YM, Herman JP. Neural regulation of endocrine and autonomic stress responses. Nat Rev Neurosci 2009, 10(6):397-409. 10.1038/nrn2647, 19469025.
4. Christoffel DJ, Golden SA, Russo SJ. Structural and synaptic plasticity in stress-related disorders. Rev Neurosci 2011, 22(5):535-549. 3212803, 21967517.
5. Popoli M, Yan Z, McEwen BS, Sanacora G. The stressed synapse: the impact of stress and glucocorticoids on glutamate transmission. Nat Rev Neurosci 2012, 13(January):22-37.
6. Sousa N, Cerqueira JJ, Almeida OF. Corticosteroid receptors and neuroplasticity. Brain Res Rev 2008, 57(2):561-570. 10.1016/j.brainresrev.2007.06.007, 17692926.
7. Castren E, Rantamaki T. The role of BDNF and its receptors in depression and antidepressant drug action: Reactivation of developmental plasticity. Dev Neurobiol 2010, 70(5):289-297. 10.1002/dneu.20758, 20186711.
8. Duman RS, Voleti B. Signaling pathways underlying the pathophysiology and treatment of depression: novel mechanisms for rapid-acting agents. Trends Neurosci 2012, 35(1):47-56. 10.1016/j.tins.2011.11.004, 22217452.
9. Pittenger C, Duman RS. Stress, depression, and neuroplasticity: a convergence of mechanisms. Neuropsychopharmacology 2008, 33(1):88-109. 10.1038/sj.npp.1301574, 17851537.
10. Hinwood M, Tynan RJ, Day TA, Walker FR. Repeated social defeat selectively increases deltaFosB expression and histone H3 acetylation in the infralimbic medial prefrontal cortex. Cereb Cortex 2011, 21(2):262-271. 10.1093/cercor/bhq080, 20513656.
11. Koenigs M, Young L, Adolphs R, Tranel D, Cushman F, Hauser M, Damasio A. Damage to the prefrontal cortex increases utilitarian moral judgements. Nature 2007, 446(7138):908-911. 10.1038/nature05631, 2244801, 17377536.
12. Mayberg HS, Lozano AM, Voon V, McNeely HE, Seminowicz D, Hamani C, Schwalb JM, Kennedy SH. Deep brain stimulation for treatment-resistant depression. Neuron 2005, 45(5):651-660. 10.1016/j.neuron.2005.02.014, 15748841.
13. Kennedy SH, Giacobbe P, Rizvi SJ, Placenza FM, Nishikawa Y, Mayberg HS, Lozano AM. Deep brain stimulation for treatment-resistant depression: follow-up after 3 to 6 years. Am J Psychiatry 2011, 168(5):502-510. 10.1176/appi.ajp.2010.10081187, 21285143.
14. Krishnan V, Han MH, Graham DL, Berton O, Renthal W, Russo SJ, Laplant Q, Graham A, Lutter M, Lagace DC, et al. Molecular adaptations underlying susceptibility and resistance to social defeat in brain reward regions. Cell 2007, 131(2):391-404. 10.1016/j.cell.2007.09.018, 17956738.
15. Mirnics K, Pevsner J. Progress in the use of microarray technology to study the neurobiology of disease. Nat Neurosci 2004, 7(5):434-439. 10.1038/nn1230, 15114354.
16. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Royal Statistical Soc 1995, 57(1):289-300.
17. Dennis G, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA. DAVID: database for annotation, visualization, and integrated discovery. Genome Biol 2003, 4(5):P3. 10.1186/gb-2003-4-5-p3, 12734009.
18. Shansky RM, Hamo C, Hof PR, McEwen BS, Morrison JH. Stress-induced dendritic remodeling in the prefrontal cortex is circuit specific. Cereb Cortex 2009, 19(10):2479-2484. 10.1093/cercor/bhp003, 2742599, 19193712.
19. Radley JJ, Rocher AB, Rodriguez A, Ehlenberger DB, Dammann M, McEwen BS, Morrison JH, Wearne SL, Hof PR. Repeated stress alters dendritic spine morphology in the rat medial prefrontal cortex. J Comp Neurol 2008, 507(1):1141-1150. 10.1002/cne.21588, 2796421, 18157834.
20. Brown SM, Henning S, Wellman CL. Mild, short-term stress alters dendritic morphology in rat medial prefrontal cortex. Cereb Cortex 2005, 15(11):1714-1722. 10.1093/cercor/bhi048, 15703248.
21. Goldwater DS, Pavlides C, Hunter RG, Bloss EB, Hof PR, McEwen BS, Morrison JH. Structural and functional alterations to rat medial prefrontal cortex following chronic restraint stress and recovery. Neuroscience 2009, 164(2):798-808. 10.1016/j.neuroscience.2009.08.053, 2762025, 19723561.
22. Liston C, Miller MM, Goldwater DS, Radley JJ, Rocher AB, Hof PR, Morrison JH, McEwen BS. Stress-induced alterations in prefrontal cortical dendritic morphology predict selective impairments in perceptual attentional set-shifting. J Neurosci 2006, 26(30):7870-7874. 10.1523/JNEUROSCI.1184-06.2006, 16870732.
23. Hinwood M, Morandini J, Day TA, Walker FR. Evidence that microglia mediate the neurobiological effects of chronic psychological stress on the medial prefrontal cortex. Cereb Cortex 2012, 22(6):1442-1454. 10.1093/cercor/bhr229, 21878486.
24. Chana G, Landau S, Beasley C, Everall IP, Cotter D. Two-dimensional assessment of cytoarchitecture in the anterior cingulate cortex in major depressive disorder, bipolar disorder, and schizophrenia: evidence for decreased neuronal somal size and increased neuronal density. Biol Psychiatry 2003, 53(12):1086-1098. 10.1016/S0006-3223(03)00114-8, 12814860.
25. Frodl TS, Koutsouleris N, Bottlender R, Born C, Jager M, Scupin I, Reiser M, Moller HJ, Meisenzahl EM. Depression-related variation in brain morphology over 3 years: effects of stress?. Arch Gen Psychiatry 2008, 65(10):1156-1165. 10.1001/archpsyc.65.10.1156, 18838632.
26. Drevets WC, Price JL, Simpson JR, Todd RD, Reich T, Vannier M, Raichle ME. Subgenual prefrontal cortex abnormalities in mood disorders. Nature 1997, 386(6627):824-827. 10.1038/386824a0, 9126739.
27. Kim KS, Han PL. Optimization of chronic stress paradigms using anxiety- and depression-like behavioral parameters. J Neurosci Res 2006, 83(3):497-507. 10.1002/jnr.20754, 16416425.
28. McLaughlin KJ, Gomez JL, Baran SE, Conrad CD. The effects of chronic stress on hippocampal morphology and function: an evaluation of chronic restraint paradigms. Brain Res 2007, 1161:56-64. 2667378, 17603026.
29. Berton O, McClung CA, Dileone RJ, Krishnan V, Renthal W, Russo SJ, Graham D, Tsankova NM, Bolanos CA, Rios M, et al. Essential role of BDNF in the mesolimbic dopamine pathway in social defeat stress. Science 2006, 311(5762):864-868. 10.1126/science.1120972, 16469931.
30. Tsankova NM, Berton O, Renthal W, Kumar A, Neve RL, Nestler EJ. Sustained hippocampal chromatin regulation in a mouse model of depression and antidepressant action. Nat Neurosci 2006, 9(4):519-525. 10.1038/nn1659, 16501568.
31. Avgustinovich DF, Kovalenko IL, Kudryavtseva NN. A model of anxious depression: persistence of behavioral pathology. Neurosci Behav Physiol 2005, 35(9):917-924. 10.1007/s11055-005-0146-6, 16270173.
32. Willner P. Chronic mild stress (CMS) revisited: consistency and behavioural-neurobiological concordance in the effects of CMS. Neuropsychobiology 2005, 52(2):90-110. 10.1159/000087097, 16037678.
33. Adamec RE, Blundell J, Burton P. Neural circuit changes mediating lasting brain and behavioral response to predator stress. Neurosci Biobehav Rev 2005, 29(8):1225-1241. 10.1016/j.neubiorev.2005.05.007, 16099042.
34. Berton O, Durand M, Aguerre S, Mormede P, Chaouloff F. Behavioral, neuroendocrine and serotonergic consequences of single social defeat and repeated fluoxetine pretreatment in the Lewis rat strain. Neuroscience 1999, 92(1):327-341. 10.1016/S0306-4522(98)00742-8, 10392854.
35. Mitra R, Jadhav S, McEwen BS, Vyas A, Chattarji S. Stress duration modulates the spatiotemporal patterns of spine formation in the basolateral amygdala. Proc Natl Acad Sci USA 2005, 102(26):9371-9376. 10.1073/pnas.0504011102, 1166638, 15967994.
36. Chiba S, Numakawa T, Ninomiya M, Richards MC, Wakabayashi C, Kunugi H. Chronic restraint stress causes anxiety- and depression-like behaviors, downregulates glucocorticoid receptor expression, and attenuates glutamate release induced by brain-derived neurotrophic factor in the prefrontal cortex. Prog Neuropsychopharmacol Biol Psychiatry 2012, 39(1):112-119. 10.1016/j.pnpbp.2012.05.018, 22664354.
37. Bland ST, Tamlyn JP, Barrientos RM, Greenwood BN, Watkins LR, Campeau S, Day HE, Maier SF. Expression of fibroblast growth factor-2 and brain-derived neurotrophic factor mRNA in the medial prefrontal cortex and hippocampus after uncontrollable or controllable stress. Neuroscience 2007, 144(4):1219-1228. 10.1016/j.neuroscience.2006.11.026, 1904349, 17197100.
38. Duric V, Banasr M, Licznerski P, Schmidt HD, Stockmeier CA, Simen AA, Newton SS, Duman RS. A negative regulator of MAP kinase causes depressive behavior. Nat Med 2010, 16(11):1328-1332. 10.1038/nm.2219, 3066515, 20953200.
39. Surget A, Wang Y, Leman S, Ibarguen-Vargas Y, Edgar N, Griebel G, Belzung C, Sibille E. Corticolimbic transcriptome changes are state-dependent and region-specific in a rodent model of depression and of antidepressant reversal. Neuropsychopharmacology 2009, 34(6):1363-1380. 10.1038/npp.2008.76, 2669699, 18536703.
40. Karssen AM, Her S, Li JZ, Patel PD, Meng F, Bunney WE, Jones EG, Watson SJ, Akil H, Myers RM, et al. Stress-induced changes in primate prefrontal profiles of gene expression. Mol Psychiatry 2007, 12(12):1089-1102. 10.1038/sj.mp.4002095, 17893703.
41. Orsetti M, Di Brisco F, Canonico PL, Genazzani AA, Ghi P. Gene regulation in the frontal cortex of rats exposed to the chronic mild stress paradigm, an animal model of human depression. Eur J Neurosci 2008, 27(8):2156-2164. 10.1111/j.1460-9568.2008.06155.x, 18371075.
42. da Huang W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 2009, 4(1):44-57.
43. Huang Da W, Sherman BT, Zheng X, Yang J, Imamichi T, Stephens R, Lempicki RA. Extracting biological meaning from large gene lists with DAVID. Curr Protoc Bioinformatics 2009, Chapter 13:Unit 13 11.
44. Dwivedi Y, Rizavi HS, Conley RR, Roberts RC, Tamminga CA, Pandey GN. Altered gene expression of brain-derived neurotrophic factor and receptor tyrosine kinase B in postmortem brain of suicide subjects. Arch Gen Psychiatry 2003, 60(8):804-815. 10.1001/archpsyc.60.8.804, 12912764.
45. Guilloux JP, Douillard-Guilloux G, Kota R, Wang X, Gardier AM, Martinowich K, Tseng GC, Lewis DA, Sibille E. Molecular evidence for BDNF- and GABA-related dysfunctions in the amygdala of female subjects with major depression. Mol Psychiatry 2011, [Epub ahead of print]..
46. Bocchio-Chiavetto L, Bagnardi V, Zanardini R, Molteni R, Nielsen MG, Placentino A, Giovannini C, Rillosi L, Ventriglia M, Riva MA, et al. Serum and plasma BDNF levels in major depression: a replication study and meta-analyses. World J Biol Psychiatry 2010, 11(6):763-773. 10.3109/15622971003611319, 20334574.
47. Fuchikami M, Morinobu S, Segawa M, Okamoto Y, Yamawaki S, Ozaki N, Inoue T, Kusumi I, Koyama T, Tsuchiyama K, et al. DNA methylation profiles of the brain-derived neurotrophic factor (BDNF) gene as a potent diagnostic biomarker in major depression. PLoS One 2011, 6(8):e23881. 10.1371/journal.pone.0023881, 3166055, 21912609.
48. Roceri M, Cirulli F, Pessina C, Peretto P, Racagni G, Riva MA. Postnatal repeated maternal deprivation produces age-dependent changes of brain-derived neurotrophic factor expression in selected rat brain regions. Biol Psychiatry 2004, 55(7):708-714. 10.1016/j.biopsych.2003.12.011, 15038999.
49. Naert G, Ixart G, Maurice T, Tapia-Arancibia L, Givalois L. Brain-derived neurotrophic factor and hypothalamic-pituitary-adrenal axis adaptation processes in a depressive-like state induced by chronic restraint stress. Mol Cell Neurosci 2011, 46(1):55-66. 10.1016/j.mcn.2010.08.006, 20708081.
50. Zhang Y, Gu F, Chen J, Dong W. Chronic antidepressant administration alleviates frontal and hippocampal BDNF deficits in CUMS rat. Brain Res 2010, 1366:141-148.
51. Altar CA, Cai N, Bliven T, Juhasz M, Conner JM, Acheson AL, Lindsay RM, Wiegand SJ. Anterograde transport of brain-derived neurotrophic factor and its role in the brain. Nature 1997, 389(6653):856-860. 10.1038/39885, 9349818.
52. Smith MA, Makino S, Altemus M, Michelson D, Hong SK, Kvetnansky R, Post RM. Stress and antidepressants differentially regulate neurotrophin 3 mRNA expression in the locus coeruleus. Proc Natl Acad Sci USA 1995, 92(19):8788-8792. 10.1073/pnas.92.19.8788, 41052, 7568018.
53. Roceri M, Hendriks W, Racagni G, Ellenbroek BA, Riva MA. Early maternal deprivation reduces the expression of BDNF and NMDA receptor subunits in rat hippocampus. Mol Psychiatry 2002, 7(6):609-616. 10.1038/sj.mp.4001036, 12140784.
54. Marmigere F, Givalois L, Rage F, Arancibia S, Tapia-Arancibia L. Rapid induction of BDNF expression in the hippocampus during immobilization stress challenge in adult rats. Hippocampus 2003, 13(5):646-655. 10.1002/hipo.10109, 12921353.
55. Rage F, Givalois L, Marmigere F, Tapia-Arancibia L, Arancibia S. Immobilization stress rapidly modulates BDNF mRNA expression in the hypothalamus of adult male rats. Neuroscience 2002, 112(2):309-318. 10.1016/S0306-4522(02)00072-6, 12044449.
56. Huang EJ, Reichardt LF. Trk receptors: roles in neuronal signal transduction. Annu Rev Biochem 2003, 72:609-642. 10.1146/annurev.biochem.72.121801.161629, 12676795.
57. Minichiello L. TrkB signalling pathways in LTP and learning. Nat Rev Neurosci 2009, 10(12):850-860. 10.1038/nrn2738, 19927149.
58. Genoud C, Knott GW, Sakata K, Lu B, Welker E. Altered synapse formation in the adult somatosensory cortex of brain-derived neurotrophic factor heterozygote mice. J Neurosci 2004, 24(10):2394-2400. 10.1523/JNEUROSCI.4040-03.2004, 15014114.
59. Minichiello L, Korte M, Wolfer D, Kuhn R, Unsicker K, Cestari V, Rossi-Arnaud C, Lipp HP, Bonhoeffer T, Klein R. Essential role for TrkB receptors in hippocampus-mediated learning. Neuron 1999, 24(2):401-414. 10.1016/S0896-6273(00)80853-3, 10571233.
60. Abidin I, Kohler T, Weiler E, Zoidl G, Eysel UT, Lessmann V, Mittmann T. Reduced presynaptic efficiency of excitatory synaptic transmission impairs LTP in the visual cortex of BDNF-heterozygous mice. Eur J Neurosci 2006, 24(12):3519-3531. 10.1111/j.1460-9568.2006.05242.x, 17229100.
61. Chourbaji S, Brandwein C, Vogt MA, Dormann C, Hellweg R, Gass P. Nature vs. nurture: can enrichment rescue the behavioural phenotype of BDNF heterozygous mice?. Behav Brain Res 2008, 192(2):254-258. 10.1016/j.bbr.2008.04.015, 18538870.
62. Sengupta S, Peterson TR, Sabatini DM. Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress. Mol Cell 2010, 40(2):310-322. 10.1016/j.molcel.2010.09.026, 2993060, 20965424.
63. Hoeffer CA, Klann E. mTOR signaling: at the crossroads of plasticity, memory and disease. Trends Neurosci 2010, 33(2):67-75. 10.1016/j.tins.2009.11.003, 2821969, 19963289.
64. Jope RS. Glycogen synthase kinase-3 in the etiology and treatment of mood disorders. Front Mol Neurosci 2011, 4:16. 3152743, 21886606.
65. Beurel E, Song L, Jope RS. Inhibition of glycogen synthase kinase-3 is necessary for the rapid antidepressant effect of ketamine in mice. Mol Psychiatry 2011, 16(11):1068-1070. 10.1038/mp.2011.47, 3200424, 21502951.
66. Karege F, Perroud N, Burkhardt S, Schwald M, Ballmann E, La Harpe R, Malafosse A. Alteration in kinase activity but not in protein levels of protein kinase B and glycogen synthase kinase-3beta in ventral prefrontal cortex of depressed suicide victims. Biol Psychiatry 2007, 61(2):240-245. 10.1016/j.biopsych.2006.04.036, 16876135.
67. Li X, Zhu W, Roh MS, Friedman AB, Rosborough K, Jope RS. In vivo regulation of glycogen synthase kinase-3beta (GSK3beta) by serotonergic activity in mouse brain. Neuropsychopharmacology 2004, 29(8):1426-1431. 10.1038/sj.npp.1300439, 1986663, 15039769.
68. Wilkinson MB, Dias C, Magida J, Mazei-Robison M, Lobo M, Kennedy P, Dietz D, Covington H, Russo S, Neve R, et al. A novel role of the WNT-dishevelled-GSK3beta signaling cascade in the mouse nucleus accumbens in a social defeat model of depression. J Neurosci 2011, 31(25):9084-9092. 10.1523/JNEUROSCI.0039-11.2011, 3133937, 21697359.
69. Jope RS, Johnson GV. The glamour and gloom of glycogen synthase kinase-3. Trends Biochem Sci 2004, 29(2):95-102. 10.1016/j.tibs.2003.12.004, 15102436.
70. Okamoto H, Voleti B, Banasr M, Sarhan M, Duric V, Girgenti MJ, Dileone RJ, Newton SS, Duman RS. Wnt2 expression and signaling is increased by different classes of antidepressant treatments. Biol Psychiatry 2010, 68(6):521-527. 10.1016/j.biopsych.2010.04.023, 2929274, 20570247.
71. Otsuki K, Uchida S, Watanuki T, Wakabayashi Y, Fujimoto M, Matsubara T, Funato H, Watanabe Y. Altered expression of neurotrophic factors in patients with major depression. J Psychiatr Res 2008, 42(14):1145-1153. 10.1016/j.jpsychires.2008.01.010, 18313696.
72. Dwivedi Y, Rizavi HS, Zhang H, Mondal AC, Roberts RC, Conley RR, Pandey GN. Neurotrophin receptor activation and expression in human postmortem brain: effect of suicide. Biol Psychiatry 2009, 65(4):319-328. 10.1016/j.biopsych.2008.08.035, 2654767, 18930453.
73. Karege F, Vaudan G, Schwald M, Perroud N, La Harpe R. Neurotrophin levels in postmortem brains of suicide victims and the effects of antemortem diagnosis and psychotropic drugs. Brain Res Mol Brain Res 2005, 136(1-2):29-37.
74. Hock C, Heese K, Muller-Spahn F, Huber P, Riesen W, Nitsch RM, Otten U. Increased cerebrospinal fluid levels of neurotrophin 3 (NT-3) in elderly patients with major depression. Mol Psychiatry 2000, 5(5):510-513. 10.1038/sj.mp.4000743, 11032384.
75. Coppell AL, Pei Q, Zetterstrom TS. Bi-phasic change in BDNF gene expression following antidepressant drug treatment. Neuropharmacology 2003, 44(7):903-910. 10.1016/S0028-3908(03)00077-7, 12726822.
76. Nestler EJ, Hyman SE. Animal models of neuropsychiatric disorders. Nat Neurosci 2010, 13(10):1161-1169. 10.1038/nn.2647, 20877280.
77. Christiansen SH, Olesen MV, Wortwein G, Woldbye DP. Fluoxetine reverts chronic restraint stress-induced depression-like behaviour and increases neuropeptide Y and galanin expression in mice. Behav Brain Res 2011, 216(2):585-591. 10.1016/j.bbr.2010.08.044, 20816900.
78. Reid S, Barbui C. Long term treatment of depression with selective serotonin reuptake inhibitors and newer antidepressants. BMJ 2010, 340:c1468. 10.1136/bmj.c1468, 20348175.
79. McNamara RK, Able JA, Rider T, Tso P, Jandacek R. Effect of chronic fluoxetine treatment on male and female rat erythrocyte and prefrontal cortex fatty acid composition. Prog Neuropsychopharmacol Biol Psychiatry 2010, 34(7):1317-1321. 10.1016/j.pnpbp.2010.07.016, 2939160, 20655971.
80. Thompson MR, Li KM, Clemens KJ, Gurtman CG, Hunt GE, Cornish JL, McGregor IS. Chronic fluoxetine treatment partly attenuates the long-term anxiety and depressive symptoms induced by MDMA ('Ecstasy') in rats. Neuropsychopharmacology 2004, 29(4):694-704. 10.1038/sj.npp.1300347, 14627999.
81. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 6th edition 2007, London: Academic Press, Elsevier.
82. Brown AL, Smith DW. Improved RNA preservation for immunolabeling and laser microdissection. RNA 2009, 15(12):2364-2374. 10.1261/rna.1733509, 2779672, 19850907.
83. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 2008, 3(6):1101-1108. 10.1038/nprot.2008.73, 18546601.