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Volumn 16, Issue 4, 2005, Pages 334-338

Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites

Author keywords

Close proximity sites; Combination primer; Multi site mutagenesis

Indexed keywords


EID: 84867493191     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (7)

References (9)
  • 1
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    • A general method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interactions
    • Higuchi R, Krummel B, Saiki RK. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 1988;16:7351-7367.
    • (1988) Nucleic Acids Res , vol.16 , pp. 7351-7367
    • Higuchi, R.1    Krummel, B.2    Saiki, R.K.3
  • 2
    • 0024520745 scopus 로고
    • Site-directed mutagenesis by overlap extension using the polymerase chain reaction
    • Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 1989;77:51-59.
    • (1989) Gene , vol.77 , pp. 51-59
    • Ho, S.N.1    Hunt, H.D.2    Horton, R.M.3    Pullen, J.K.4    Pease, L.R.5
  • 3
    • 0025325983 scopus 로고
    • The "Megaprimer" method of site-directed mutagenesis
    • Sarkar G, Sommer SS. The "Megaprimer" method of site-directed mutagenesis. BioTechniques 1990;8:404-407.
    • (1990) BioTechniques , vol.8 , pp. 404-407
    • Sarkar, G.1    Sommer, S.S.2
  • 4
    • 0034662681 scopus 로고    scopus 로고
    • Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis
    • Sawano A, Miyawaki A. Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis. Nucleic Acids Res 2000;28:e78.
    • (2000) Nucleic Acids Res , vol.28
    • Sawano, A.1    Miyawaki, A.2
  • 5
    • 0033951912 scopus 로고    scopus 로고
    • Multiple site mutagenesis with high targeting efficiency in one cloning step
    • Kim Y-G, Maas S. Multiple site mutagenesis with high targeting efficiency in one cloning step. BioTechniques 2000;28:196-198.
    • (2000) BioTechniques , vol.28 , pp. 196-198
    • Kim, Y.-G.1    Maas, S.2
  • 6
    • 0032052278 scopus 로고    scopus 로고
    • An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes
    • Kirsch RD, Joly E. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. BioTechniques 1998;26:1848-1850.
    • (1998) BioTechniques , vol.26 , pp. 1848-1850
    • Kirsch, R.D.1    Joly, E.2
  • 7
    • 3843146246 scopus 로고    scopus 로고
    • An efficient one-step site-directed and site-saturation mutagenesis protocol
    • Zheng L, Baumann U, Reymond J-L. An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res 2004;32:e115.
    • (2004) Nucleic Acids Res , vol.32
    • Zheng, L.1    Baumann, U.2    Reymond, J.-L.3
  • 8
    • 0346362261 scopus 로고    scopus 로고
    • Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round
    • Seyfang A, Jin JH. Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round. Anal Biochem 2004;324:285-291.
    • (2004) Anal Biochem , vol.324 , pp. 285-291
    • Seyfang, A.1    Jin, J.H.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.