Multiple site mutagenesis with high targeting efficiency in one cloning step

BioTechniques

Volumn 28, Issue 2, 2000, Pages 196-198

  Kim, Yang Gyun ^a   Maas, Stefan ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA SEQUENCE; DNA TEMPLATE; ESCHERICHIA COLI; GENE TARGETING; GENE TECHNOLOGY; LABORATORY TEST; MOLECULAR BIOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 0033951912     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00282bm01     Document Type: Article

References (9)

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2. Ishii, T.M., P. Zerr, X.M. Xia, C.T. Bond, J. Maylie and J.P. Adelman. 1998. Site-directed mutagenesis. Methods Enzymol. 293:53-71.
3. Kirsch, R.D. and E. Joly. 1998. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids Res. 26:1848-1850.
4. Kunkel, T.A. 1985. Rapid isolation and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
5. Nelson, M. and M. McClelland. 1992. Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes. Methods Enzymol. 216:279-303.
6. Papworth, C., J.C. Bauer, J. Braman and D.A. Wright. 1996. Site-directed mutagenesis in one day with >80% efficiency. Strategies 9:3-4.
7. Sambrook, J., E. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
8. Scott, B., K. Nelson, J. Cline and K. Kretz. 1994. High-fidelity PCR amplification with Pfu DNA polymerase. Strategies 7:62-63.
9. Weiner, M.P., G.L. Costta, W. Schoettlin, J. Cline, E. Mathur and J.C. Bauer. 1994 Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction. Gene 151:119-123.