A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital

Annals of Clinical Microbiology and Antimicrobials

Volumn 9, Issue , 2010, Pages

  Hardegen, Christoph ^a   Messler, Sabine ^a   Henrich, Birgit ^a   Pfeffer, Klaus ^a   Würthner, Jens ^b   MacKenzie, Colin R ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGED; ARTICLE; ATYPICAL ENTEROPATHOGENIC ESCHERICHIA COLI; BACTERIAL GROWTH; BACTERIUM DETECTION; CHILD; CONTROLLED STUDY; DIAGNOSTIC TEST; DIARRHEAGENIC ESCHERICHIA COLI; ENTEROPATHOGENIC ESCHERICHIA COLI; FECES CULTURE; HUMAN; INFECTIOUS DIARRHEA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRESCHOOL CHILD; PREVALENCE; REAL TIME POLYMERASE CHAIN REACTION; SCHOOL CHILD; SEQUENCE ANALYSIS; UNIVERSITY HOSPITAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTION; EVALUATION; FECES; GENETICS; GERMANY; INFANT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; NEWBORN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

PRIMER DNA;

ADOLESCENT; ADULT; AGED; CHILD; CHILD, PRESCHOOL; DNA PRIMERS; ENTEROPATHOGENIC ESCHERICHIA COLI; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FECES; GERMANY; HOSPITALS, UNIVERSITY; HUMANS; INFANT; INFANT, NEWBORN; MIDDLE AGED; POLYMERASE CHAIN REACTION; PREVALENCE; SENSITIVITY AND SPECIFICITY;

EID: 76949092949     PISSN: None     EISSN: 14760711     Source Type: Journal    
DOI: 10.1186/1476-0711-9-5     Document Type: Article

References (31)

1. Cheng AC, McDonald JR, Thielman NM. Infectious diarrhea in developed and developing countries. J Clin Gastroenterol 2005, 39:757-773. 10.1097/01.mcg.0000177231.13770.07, 16145337.
2. Bryce J, Boschi-Pinto C, Shibuya K, Black RE. WHO estimates of the causes of death in children. Lancet 2005, 365:1147-1152. 10.1016/S0140-6736(05)71877-8, 15794969.
3. Olesen B, Neimann J, Bottiger B, Ethelberg S, Schiellerup P, Jensen C, Helms M, Scheutz F, Olsen KE, Krogfelt K, et al. Etiology of diarrhea in young children in Denmark: a case-control study. J Clin Microbiol 2005, 43:3636-3641. 10.1128/JCM.43.8.3636-3641.2005, 1234006, 16081890.
4. Cohen MB, Nataro JP, Bernstein DI, Hawkins J, Roberts N, Staat MA. Prevalence of diarrheagenic Escherichia coli in acute childhood enteritis: a prospective controlled study. J Pediatr 2005, 146:54-61. 10.1016/j.jpeds.2004.08.059, 15644823.
5. Nataro JP, Mai V, Johnson J, Blackwelder WC, Heimer R, Tirrell S, Edberg SC, Braden CR, Glenn MJ, Hirshon JM. Diarrheagenic Escherichia coli infection in Baltimore, Maryland, and New Haven, Connecticut. Clin Infect Dis 2006, 43:402-407. 10.1086/505867, 16838226.
6. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004, 2:123-140. 10.1038/nrmicro818, 15040260.
7. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998, 11:142-201. 121379, 9457432.
8. Nataro JP, Kaper JB, Robins BR, Prado V, Vial P, Levine MM. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J 1987, 6:829-831.
9. Huang DB, Nataro JP, DuPont HL, Kamat PP, Mhatre AD, Okhuysen PC, Chiang T. Enteroaggregative Escherichia coli is a cause of acute diarrheal illness: a meta-analysis. Clin Infect Dis 2006, 43:556-563. 10.1086/505869, 16886146.
10. Steiner TS, Lima AA, Nataro JP, Guerrant RL. Enteroaggregative Escherichia coli produce intestinal inflammation and growth impairment and cause interleukin-8 release from intestinal epithelial cells. J Infect Dis 1998, 177:88-96. 10.1086/513809, 9419174.
11. Bouckenooghe AR, DuPont HL, Jiang ZD, Adachi J, Mathewson JJ, Verenkar MP, Rodrigues S, Steffen R. Markers of enteric inflammation in enteroaggregative Escherichia coli diarrhea in travelers. Am J Trop Med Hyg 2000, 62:711-713.
12. Greenberg DE, Jiang ZD, Steffen R, Verenker MP, DuPont HL. Markers of inflammation in bacterial diarrhea among travelers, with a focus on enteroaggregative Escherichia coli pathogenicity. J Infect Dis 2002, 185:944-949. 10.1086/339617, 11920319.
13. Bischoff C, Luthy J, Altwegg M, Baggi F. Rapid detection of diarrheagenic E. coli by real-time PCR. J Microbiol Methods 2005, 61:335-341. 10.1016/j.mimet.2004.12.007, 15767009.
14. Aranda KR, Fagundes-Neto U, Scaletsky IC. Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp. J Clin Microbiol 2004, 42:5849-5853. 10.1128/JCM.42.12.5849-5853.2004, 535216, 15583323.
15. Brandal LT, Lindstedt BA, Aas L, Stavnes TL, Lassen J, Kapperud G. Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp. J Microbiol Methods 2007, 68:331-341. 10.1016/j.mimet.2006.09.013, 17079041.
16. Kimata K, Shima T, Shimizu M, Tanaka D, Isobe J, Gyobu Y, Watahiki M, Nagai Y. Rapid categorization of pathogenic Escherichia coli by multiplex PCR. Microbiol Immunol 2005, 49:485-492.
17. Pass MA, Odedra R, Batt RM. Multiplex PCRs for identification of Escherichia coli virulence genes. J Clin Microbiol 2000, 38:2001-2004. 86652, 10790141.
18. Vidal M, Kruger E, Duran C, Lagos R, Levine M, Prado V, Toro C, Vidal R. Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections. J Clin Microbiol 2005, 43:5362-5365. 10.1128/JCM.43.10.5362-5365.2005, 1248459, 16208019.
19. Huppertz HI, Rutkowski S, Aleksic S, Karch H. Acute and chronic diarrhoea and abdominal colic associated with enteroaggregative Escherichia coli in young children living in western Europe. Lancet 1997, 349:1660-1662. 10.1016/S0140-6736(96)12485-5, 9186384.
20. Kozub-Witkowski E, Krause G, Frankel G, Kramer D, Appel B, Beutin L. Serotypes and virutypes of enteropathogenic and enterohaemorrhagic Escherichia coli strains from stool samples of children with diarrhoea in Germany. J Appl Microbiol 2008, 104:403-410.
21. Pabst WL, Altwegg M, Kind C, Mirjanic S, Hardegger D, Nadal D. Prevalence of enteroaggregative Escherichia coli among children with and without diarrhea in Switzerland. J Clin Microbiol 2003, 41:2289-2293. 10.1128/JCM.41.6.2289-2293.2003, 156476, 12791838.
22. Franke J, Franke S, Schmidt H, Schwarzkopf A, Wieler LH, Baljer G, Beutin L, Karch H. Nucleotide sequence analysis of enteropathogenic Escherichia coli (EPEC) adherence factor probe and development of PCR for rapid detection of EPEC harboring virulence plasmids. J Clin Microbiol 1994, 32:2460-2463. 264083, 7814482.
23. Schmidt H, Knop C, Franke S, Aleksic S, Heesemann J, Karch H. Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol 1995, 33:701-705. 228017, 7751380.
24. Wilde J, Eiden J, Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol 1990, 28:1300-1307. 267924, 1696283.
25. Rosenstraus M, Wang Z, Chang SY, DeBonville D, Spadoro JP. An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance. J Clin Microbiol 1998, 36:191-197. 124832, 9431945.
26. Persson S, Olsen KE, Scheutz F, Krogfelt KA, Gerner-Smidt P. A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory. Clin Microbiol Infect 2007, 13:516-524. 10.1111/j.1469-0691.2007.01692.x, 17331124.
27. Ochoa TJ, Barletta F, Contreras C, Mercado E. New insights into the epidemiology of enteropathogenic Escherichia coli infection. Trans R Soc Trop Med Hyg 2008, 102:852-856. 10.1016/j.trstmh.2008.03.017, 2575077, 18455741.
28. Prere MF, Bacrie SC, Baron O, Fayet O. Bacterial aetiology of diarrhoea in young children: high prevalence of enteropathogenic Escherichia coli (EPEC) not belonging to the classical EPEC serogroups. Pathol Biol (Paris) 2006, 54:600-602.
29. Trabulsi LR, Keller R, Tardelli Gomes TA. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 2002, 8:508-513. 2732489, 11996687.
30. Afset JE, Bevanger L, Romundstad P, Bergh K. Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhoea. J Med Microbiol 2004, 53:1137-1144. 10.1099/jmm.0.45719-0, 15496393.
31. Bielaszewska M, Middendorf B, Kock R, Friedrich AW, Fruth A, Karch H, Schmidt MA, Mellmann A. Shiga toxin-negative attaching and effacing Escherichia coli: distinct clinical associations with bacterial phylogeny and virulence traits and inferred in-host pathogen evolution. Clin Infect Dis 2008, 47:208-217. 10.1086/589245, 18564929.