Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins

Journal of Clinical Microbiology

Volumn 47, Issue 4, 2009, Pages 1218-1220

  Rodas, Claudia ^a,b,d   Iniguez, Volga ^b   Qadri, Firdausi ^c   Wiklund, Gudrun ^a   Svennerholm, Ann Mari ^a   Sjoling, Åsa ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b UNIVERSIDAD MAYOR DE SAN ANDRÉS   (Bolivia)

^c INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH   (Bangladesh)

^d UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; ESCHERICHIA COLI ENTEROTOXIN; VIRULENCE FACTOR; BACTERIAL TOXIN; ESCHERICHIA COLI PROTEIN;

ARTICLE; BACTERIAL COLONIZATION; BANGLADESH; BOLIVIA; CLINICAL LABORATORY; DIAGNOSTIC PROCEDURE; ESCHERICHIA COLI; GEOGRAPHIC DISTRIBUTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; COMPARATIVE STUDY; ENTEROTOXIGENIC ESCHERICHIA COLI; EVALUATION; GENETICS; HUMAN; METHODOLOGY; PATHOGENICITY;

ESCHERICHIA COLI;

BACTERIAL TOXINS; BANGLADESH; BOLIVIA; ENTEROTOXIGENIC ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; POLYMERASE CHAIN REACTION; VIRULENCE FACTORS;

EID: 65249135466     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00316-09     Document Type: Article

References (15)

1. Bölin, I., G. Wiklund, F. Qadri, O. Torres, A. L. Bourgeois, S. Savarino, and A.-M. Svennerholm. 2006. Enterotoxigenic Escherichia coli with STh and STp genotypes is associated with diarrhea both in children in areas of endemicity and in travelers. J. Clin. Microbiol. 44:3872-3877.
2. Gaastra, W., and A. M. Svennerholm. 1996. Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol. 4:444-452.
3. Gutiérrez-Cázarez, Z., F. Qadri, M. J. Albert, and J. A. Girón. 2000. Identification of enterotoxigenic Escherichia coli harboring longus type IV pilus gene by DNA amplification. J. Clin. Microbiol. 38:1767-1771.
4. Pichel, M. G., N. Binsztein, F. Qadri, and J. A. Giron. 2002. Type IV longus pilus of enterotoxigenic Escherichia coli: occurrence and association with toxin types and colonization factors among strains isolated in Argentina. J. Clin. Microbiol. 40:694-697.
5. Qadri, F., S. K. Das, A. S. Faruque, G. J. Fuchs, M. J. Albert, R. B. Sack, and A. M. Svennerholm. 2000. Prevalence of toxin types and colonization factors in enterotoxigenic Escherichia coli isolated during a 2-year period from diarrheal patients in Bangladesh. J. Clin. Microbiol. 38:27-31.
6. Qadri, F., A. Saha, T. Ahmed, A. Al Tarique, Y. A. Begum, and A. M. Svennerholm. 2007. Disease burden due to enterotoxigenic Escherichia coli in the first 2 years of life in an urban community in Bangladesh. Infect. Immun. 75:3961-3968.
7. Qadri, F., A. M. Svennerholm, A. S. Faruque, and R. B. Sack. 2005. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin. Microbiol. Rev. 18:465-483.
8. Rao, M. R., R. Abu-Elyazeed, S. J. Savarino, A. B. Naficy, T. F. Wierzba, I. Abdel-Messih, H. Shaheen, R. W. Frenck, Jr., A. M. Svennerholm, and J. D. Clemens. 2003. High disease burden of diarrhea due to enterotoxigenic Escherichia coli among rural Egyptian infants and young children. J. Clin. Microbiol. 41:4862-4864.
9. Roy, K., D. Hamilton, K. P. Allen, M. P. Randolph, and J. M. Fleckenstein. 2008. The EtpA exoprotein of enterotoxigenic Escherichia coli promotes intestinal colonization and is a protective antigen in an experimental model of murine infection. Infect. Immun. 76:2106-2112.
10. Shaheen, H. I., I. A. Abdel Messih, J. D. Klena, A. Mansour, Z. El-Wakkeel, T. F. Wierzba, J. W. Sanders, S. B. Khalil, D. M. Rockabrand, M. R. Monteville, P. J. Rozmajzl, A. M. Svennerholm, and R. W. Frenck. 2009. Phenotypic and genotypic analysis of enterotoxigenic Escherichia coli (ETEC) in samples obtained from Egyptian children presenting to referral hospitals. J. Clin. Microbiol. 47:189-197.
11. Sjöling, Å., G. Wiklund, S. J. Savarino, D. I. Cohen, and A. M. Svennerholm. 2007. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli toxins and colonization factors. J. Clin. Microbiol. 45:3295-3301.
12. Sommerfelt, H., H. Steinsland, H. M. Grewal, G. I. Viboud, N. Bhandari, W. Gaastra, A. M. Svennerholm, and M. K. Bhan. 1996. Colonization factors of enterotoxigenic Escherichia coli isolated from children in north India. J. Infect. Dis. 174:768-776.
13. Steinsland, H., P. Valentiner-Branth, H. M. Grewal, W. Gaastra, K. K. Molbak, and H. Sommerfelt. 2003. Development and evaluation of genotypic assays for the detection and characterization of enterotoxigenic Escherichia coli. Diagn. Microbiol. Infect. Dis. 45:97-105.
14. Svennerholm, A. M., and J. Tobias. 2008. Vaccines against enterotoxigenic Escherichia coli. Expert Rev. Vaccines 7:795-804.
15. Walker, R. I., D. Steele, and T. Aguado. 2007. Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease. Vaccine 25:2545-2566.