Oriented covalent immobilization of antibodies onto heterofunctional agarose supports: A highly efficient immuno-affinity chromatography platform

Journal of Chromatography A

Volumn 1262, Issue , 2012, Pages 56-63

  Batalla, Pilar ^a   Bolívar, Juan M ^a   Lopez Gallego, Fernando ^a   Guisan, Jose M ^a  

^a CSIC   (Spain)

Author keywords

Antibody activity; Antibody conjugation; AntiHRP IgG; Immunobiosensors; Protein distribution inside porous supports

Indexed keywords

AGAROSE; ALKALINE CONDITIONS; ANTIBODY CONJUGATION; ANTIHRP-IGG; BINDING ACTIVITIES; BIOCONJUGATES; BIOLOGICAL RECOGNITION; COORDINATION BONDS; COVALENT ATTACHMENT; COVALENT IMMOBILIZATION; FC REGION; GLYOXYL; IMMUNOBIOSENSORS; INERT SOLIDS; LYSINE RESIDUES; OPTIMAL PROTOCOLS; POROUS SUPPORT; SPECIFIC SURFACE;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANTIGENS; BIOMOLECULES; CHELATION; OPTIMIZATION; SILVER;

ANTIBODIES;

AGAROSE; FC RECEPTOR; FUNCTIONAL GROUP; GLYOXYL GROUP; IMIDAZOLE; IMMUNOGLOBULIN G; LYSINE; METAL CHELATE; SILVER; UNCLASSIFIED DRUG;

ALKALINITY; AMINO ACID SEQUENCE; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COVALENT BOND; IMMUNOAFFINITY CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PURIFICATION; SURFACE PROPERTY;

ANTIBODIES, IMMOBILIZED; BIOSENSING TECHNIQUES; BIOTECHNOLOGY; CHELATING AGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ASSAYS; HORSERADISH PEROXIDASE; IMMUNOCHROMATOGRAPHY; IMMUNOGLOBULIN G; KINETICS; MODELS, MOLECULAR; POROSITY; PROTEIN BINDING; SEPHAROSE; SILVER;

EID: 84867222939     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.08.058     Document Type: Article

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