Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Sumitomo, Yasuhiko ^a,b   Higashitsuji, Hiroaki ^a   Higashitsuji, Hisako ^a   Liu, Yu ^a   Fujita, Takanori ^a   Sakurai, Toshiharu ^c   Candeias, Marco M ^a   Itoh, Katsuhiko ^a   Chiba, Tsutomu ^a   Fujita, Jun ^a  

^a KYOTO UNIVERSITY   (Japan)

^b West Hospital   (Japan)

^c KINKI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Cold shock protein; Enhancer; Recombinant protein; Stress response; Transcription factor

Indexed keywords

CELL LINES; CHLORAMPHENICOL ACETYLTRANSFERASE; CHROMATIN IMMUNOPRECIPITATION ASSAY; CMV PROMOTER; COLD SHOCK; COPY NUMBER; DOWN-REGULATION; ENHANCER; FLANKING REGIONS; HEK-293; HEK293 CELLS; IMMUNOHISTOCHEMISTRY; IN-VITRO; LOW CULTURE TEMPERATURE; MAMMALIAN CELLS; MOLECULAR MECHANISM; OVER-EXPRESSION; REGULATORY REGIONS; REGULATORY SEQUENCES; REPORTER GENE; REPORTER-GENE EXPRESSION; RNA-BINDING PROTEIN; STRESS RESPONSE; SUB-PHYSIOLOGICAL TEMPERATURE CULTURING; TRANSFECTANTS; TRANSIENT TRANSFECTION;

ANIMAL CELL CULTURE; MOLECULAR BIOLOGY; RECOMBINANT PROTEINS; RNA; TRANSCRIPTION FACTORS;

GENE EXPRESSION REGULATION;

CHLORAMPHENICOL ACETYLTRANSFERASE; COLD INDUCIBLE RNA BINDING PROTEIN; RNA BINDING PROTEIN; TRANSCRIPTION FACTOR SP1; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; COPY NUMBER VARIATION; DNA FLANKING REGION; DOWN REGULATION; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MAMMAL CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; PROTEIN EXPRESSION; REGULATORY SEQUENCE; TRANSIENT TRANSFECTION;

5' FLANKING REGION; ANIMALS; BALB 3T3 CELLS; CELL NUCLEUS; CHO CELLS; CHROMATIN IMMUNOPRECIPITATION; CRICETINAE; CRICETULUS; DOWN-REGULATION; ENHANCER ELEMENTS, GENETIC; GENE EXPRESSION; GENES, REPORTER; HEK293 CELLS; HUMANS; MICE; MUTATION; NIH 3T3 CELLS; PROTEIN BINDING; RECOMBINANT PROTEINS; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA-BINDING PROTEINS; SP1 TRANSCRIPTION FACTOR; TEMPERATURE; TRANSFECTION;

MAMMALIA;

EID: 84867193473     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-72     Document Type: Article

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