Comparative evaluation of the QIAsymphony RGQ system with the easyMAG/R-gene combination for the quantitation of cytomegalovirus DNA load in whole blood

Virology Journal

Volumn 9, Issue , 2012, Pages

  Pillet, Sylvie ^a   Bourlet, Thomas ^a   Pozzetto, Bruno ^a  

^a UNIVERSITY HOSPITAL OF SAINT ETIENNE   (France)

Author keywords

Automation; CMV; Molecular biology; Real time PCR; Viral load

Indexed keywords

VIRUS DNA;

ARTICLE; AUTOMATION; BLOOD SAMPLING; COMPARATIVE STUDY; CYTOMEGALOVIRUS; DNA EXTRACTION; HUMAN; IMMUNOCOMPROMISED PATIENT; NUCLEIC ACID AMPLIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS LOAD;

AUTOMATION, LABORATORY; BLOOD; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; FRANCE; HUMANS; IMMUNOCOMPROMISED HOST; MOLECULAR DIAGNOSTIC TECHNIQUES; VIRAL LOAD;

CYTOMEGALOVIRUS;

EID: 84867129636     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-9-231     Document Type: Article

References (22)

1. Cytomegalovirus quantitation: Where to next in optimising patient management? Atkinson C, Emery VC, J Clin Virol 2011 51 223 228 10.1016/j.jcv.2011.04.007 21620764
2. How we treat cytomegalovirus in hematopoietic cell transplant recipients. Boeckh M, Ljungman P, Blood 2009 113 5711 5719 10.1182/blood-2008-10-143560 19299333
3. Algorithm based on CMV kinetics DNA viral load for preemptive therapy initiation after hematopoietic cell transplantation. Halfon P, Berger P, Khiri H, Martineau A, Pénaranda G, Merlin M, Faucher C, J Med Virol 2011 83 490 495 10.1002/jmv.21996 21264870
4. International consensus guidelines on the management of cytomegalovirus in solid organ transplantation. Kotton CN, Kumar D, Caliendo AM, Asberg A, Chou S, Snydman DR, Allen U, Humar A, Transplantation 2010 89 779 795 10.1097/TP.0b013e3181cee42f 20224515
5. Management of CMV, HHV-6, HHV-7 and Kaposi-sarcoma herpesvirus (HHV-8) infections in patients with hematological malignancies and after SCT. Ljungman P, de la Camara R, Cordonnier C, Einsele H, Engelhard D, Reusser P, Styczynski J, Ward K, Bone Marrow Transplant 2008 42 227 240 10.1038/bmt.2008.162 18587440
6. Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients. Bravo D, Clari MÁ Costa E, Mũoz-Cobo B, Solano C, José Remigia M, Navarro D, J Clin Microbiol 2011 49 2899 2904 10.1128/JCM.00785-11 21697323
7. Clinical evaluation of a fully automated CMV PCR assay. Kerschner H, Bauer C, Schlag P, Lee S, Goedel S, Popow-Kraupp T, J Clin Virol 2011 50 281 286 10.1016/j.jcv.2010.12.010 21292548
8. Comparison of 2 highly automated nucleic acid extraction systems for quantitation of human cytomegalovirus in whole blood. Mengelle C, Mansuy J-M, Da Silva I, Davrinche C, Izopet J, Diagn Microbiol Infect Dis 2011 69 161 166 10.1016/j.diagmicrobio.2010.08.011 21251559
9. Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load. Pillet S, Bourlet T, Pozzetto B, J Clin Microbiol 2009 47 3753 3755 10.1128/JCM.01497-09 19710270
10. Probit analysis of preference data. Sakuma M, Appl Entomol Zool 2010 33 339 47
11. Comparison of the QIAsymphony automated nucleic acid extraction and PCR setup platforms with NucliSens easyMAG and Corbett CAS-1200 liquid handling station for the detection of enteric pathogens in fecal samples. van Zanten E, Wisselink GJ, de Boer W, Stoll S, Alvarez R, Kooistra-Smid AMD, J Microbiol Methods 2011 84 335 340 10.1016/j.mimet.2010.12.024 21199666
12. Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for the detection of enteric pathogens. van Zanten E, Wisselink GJ, Stoll S, Alvarez R, Kooistra-Smid AMD, J Microbiol Methods 2011 84 365 367 10.1016/j.mimet.2010.12.023 21199665
13. Performance evaluation of the Artus hepatitis C virus QS-RGQ assay. Paba P, Fabeni L, Perno CF, Ciotti M, J Virol Methods 2012 179 77 80 10.1016/j.jviromet.2011.09.024 22001273
14. Comparison of QIAsymphony automated and QIAamp manual DNA extraction systems for measuring epstein-barr virus DNA load in whole blood using real-time PCR. Laus S, Kingsley LA, Green M, Wadowsky RM, J Mol Diagn 2011 13 695 700 10.1016/j.jmoldx.2011.07.006 21889612
15. Evaluation of the QIAsymphonyTM SP and ArtusTM RealTime extraction-quantitation systems for measuring HIV-1 virus load. Sandres-Sauné K, Raymond S, Nicot F, Boineau J, Pasquier C, Izopet J, J Clin Virol 2011 52 349 352 10.1016/j.jcv.2011.08.026 21945406
16. Comparative evaluation of the QIAGEN QIAsymphony SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory. Lee AV, Atkinson C, Manuel RJ, Clark DA, J Clin Virol 2011 52 339 343 10.1016/j.jcv.2011.08.016 22014618
17. Evaluation of real-time PCR laboratory-developed tests using analyte-specific reagents for cytomegalovirus quantitation. Caliendo AM, Ingersoll J, Fox-Canale AM, Pargman S, Bythwood T, Hayden MK, Bremer JW, Lurain NS, J Clin Microbiol 2007 45 1723 1727 10.1128/JCM.02558-06 17409210 (Pubitemid 46933898)
18. Comparison of the Digene Hybrid Capture System Cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests using AcroMetrix OptiQuant CMV DNA quantitation panels and specimens from allogeneic-stem-cell transplant recipients. Hanson KE, Reller LB, Kurtzberg J, Horwitz M, Long G, Alexander BD, J Clin Microbiol 2007 45 1972 1973 10.1128/JCM.02515-06 17314226 (Pubitemid 46933936)
19. Detection of cytomegalovirus (CMV) DNA in EDTA whole-blood samples: evaluation of the quantitative artus CMV LightCycler PCR kit in conjunction with automated sample preparation. Michelin BDA, Hadzisejdic I, Bozic M, Grahovac M, Hess M, Grahovac B, Marth E, Kessler HH, J Clin Microbiol 2008 46 1241 1245 10.1128/JCM.01403-07 18272703 (Pubitemid 351563562)
20. Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR. Raggam RB, Bozic M, Salzer HJF, Hammerschmidt S, Homberg C, Ruzicka K, Kessler HH, Med Microbiol Immunol 2010 199 311 316 10.1007/s00430-010-0164-z 20559848
21. Comparison of QIAGEN automated nucleic acid extraction methods for CMV quantitative PCR testing. Miller S, Seet H, Khan Y, Wright C, Nadarajah R, Am J Clin Pathol 2010 133 558 563 10.1309/AJCPE5VZL1ONZHFJ 20231608
22. Cytomegalovirus DNA quantitation using an automated platform for nucleic Acid extraction and real-time PCR assay setup. Forman M, Wilson A, Valsamakis A, J Clin Microbiol 2011 49 2703 2705 10.1128/JCM.00721-11 21562101