Comparative evaluation of three automated systems for DNA extraction in conjunction with three commercially available real-time PCR assays for quantitation of plasma cytomegalovirus DNAemia in allogeneic stem cell transplant recipients

Journal of Clinical Microbiology

Volumn 49, Issue 8, 2011, Pages 2899-2904

  Bravo, Dayana ^a   Clari, María Ángeles ^a   Costa, Elisa ^a   Muñoz Cobo, Beatriz ^a   Solano, Carlos ^a   Remigia, María José ^a   Navarro, David ^a,b  

^a HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ADULT; AGED; ALLOGENEIC STEM CELL TRANSPLANTATION; ARTICLE; AUTOMATION; BLOOD LEVEL; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA CONTENT; DNA EXTRACTION; FEMALE; HUMAN; INTERMETHOD COMPARISON; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REFERENCE VALUE;

ADULT; AGED; AUTOMATION; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; FEMALE; HUMANS; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; STEM CELL TRANSPLANTATION; TRANSPLANTATION; TRANSPLANTATION, HOMOLOGOUS; VIRAL LOAD; VIREMIA;

CYTOMEGALOVIRUS;

EID: 79960949393     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00785-11     Document Type: Article

References (26)

1. Abbate, I., et al. 2008. Evaluation of an automated extraction system in combination with Affigene® CMV Trender for CMV DNA quantitative determination: comparison with nested PCR and pp65 antigen test. J. Virol. Methods 151:61-65.
2. Allice, T., et al. 2008. Evaluation of a novel real-time PCR system for cytomegalovirus DNA quantitation on whole blood and correlation with pp65-antigen test in guiding pre-emptive antiviral treatment. J. Virol. Methods 148:9-16. (Pubitemid 351248987)
3. Bland, J. M., and D. G. Altman. 1986. Statistical method for assessing agreement between two methods of clinical measurement. Lancet i:307-310.
4. Boom, R., et al. 2002. Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented. J. Clin. Microbiol. 40:4105-4113.
5. Caliendo, A. M., et al. 2007. Evaluation of real-time PCR laboratory-developed test using analyte-specific reagents for cytomegalovirus quantification. J. Clin. Microbiol. 45:1723-1727.
6. Caliendo, A. M., et al. 2001. Comparison of quantitative and qualitative PCR assays for cytomegalovirus DNA in plasma. J. Clin. Microbiol. 39:1334-1348.
7. Espy, M. J., et al. 2006. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin. Microbiol. Rev. 19:165-256.
8. Fryer, J. F., A. B. Heath, R. Anderson, P. D. Minor, and the collaborative study group. 2010. Collaborative study to evaluate the proposed 1st WHO International Standard for human cytomegalovirus (HCMV) for nucleic acid amplification (NAT)-based assays. WHO ECBS report no. WHO/BS/10.2138. WHO, Geneva, Switzerland. http://whaqlibdoc.who.int/hq/2010/WHO-BS-10.2138-eng.pdf.
9. Gimeno, C., et al. 2008. Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients. J. Clin. Microbiol. 46:3311-3318.
10. Gouarin, S., et al. 2007. Multicentric evaluation of a new commercial cytomegalovirus real-time PCR quantification assay. J. Virol. Methods 146:147-154. (Pubitemid 350026628)
11. Gracia-Ahufinger, I., et al. 2010. Differences in cytomegalovirus plasma viral loads measured in allogeneic hematopoietic stem cell transplant recipients using two commercial real-time PCR assays. J. Clin. Virol. 48:142-146.
12. Hanson, K. E., et al. 2007. Comparison of the Digene Hybrid capture system cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analytespecific reagent, and Qiagen RealArt CMV LightCycler PCR reagent test using AcroMetrix OptiQuant CMV DNA quantification panels and specimens from allogeneic-stem-cell transplant recipients. J. Clin. Microbiol. 45:1972-1973.
13. Kerschner, H., et al. 2011. Clinical evaluation of a fully automated CMV PCR assay. J. Clin. Virol. 50:281-286.
14. Mengelle, C., J. M. Mansuy, I. Da Silva, C. Davrinche, and J. Izopet. 2011. Comparison of 2 highly automated nucleic acid extraction systems for quantitation of human cytomegalovirus in whole blood. Diagn. Microbiol. Infect. Dis. 69:161-166.
15. Michelin, B. D., et al. 2008. Detection of cytomegalovirus (CMV) DNA in EDTA whole-blood samples: evaluation of the quantitative artus CMV LightCycler PCR kit in conjunction with automated sample preparation. J. Clin. Microbiol. 46:1241-1245.
16. Miller, S., H. Seet, Y. Khan, C. Wright, and R. Nadarajah. 2010. Comparison of QIAGEN automated nucleic acid extraction methods for CMV quantitative PCR testing. Am. J. Clin. Pathol. 133:558-563.
17. Pang, X. L., L. Chui, J. Fenton, B. LeBlanc, and J. K. Preiksaitis. 2003. Comparison of LightCycler-based PCR, COBAS Amplicor CMV monitor, and pp65 antigenemia assays for quantitative measurement of cytomegalovirus viral load in peripheral blood specimens from patients after solid organ transplantation. J. Clin. Microbiol. 41:3167-3174.
18. Pang, X. L., et al. 2009. Interlaboratory comparison of cytomegalovirus viral load assays. Am. J. Transpl. 9:258-268.
19. Piiparinen, H., K. Höckerstedt, C. Grönhagen-Riska, and I. Lautenschlager. 2004. Comparison of two quantitative CMV PCR tests, Cobas Amplicor CMV Monitor and TaqMan assay, and pp65-antigenemia assay in the determination of viral loads from peripheral blood of organ transplant patients. J. Clin. Virol. 30:258-266.
20. Pillet, S., T. Bourlet, and B. Pozzetto. 2009. Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of Epstein-Barr virus and cytomegalovirus load. J. Clin. Microbiol. 47:3753-3755.
21. Pumannova, M., K. Roubalova, A. Vitek, and J. Sajdova. 2006. Comparison of quantitative competitive PCR-enzyme-linked immunosorbent assay with LightCycler-based PCR for measuring cytomegalovirus DNA in patients after hematopoietic stem cell transplantation. Diagn. Microbiol. Infect. Dis. 54:115-120.
22. Razonable, R. R., et al. 2001. Comparative quantitation of cytomegalovirus (CMV) DNA in solid organ transplant recipients with CMV infection by using two high-throughput automated systems. J. Clin. Microbiol. 39:4472-4476.
23. Solano, C., and D. Navarro. 2010. Clinical virology of cytomegalovirus infection following hematopoietic transplantation. Future Virol. 5:111-124.
24. Vincent, E., et al. 2009. Detection of cytomegalovirus in whole blood using three different real-time PCR chemistries. J. Mol. Diagn. 11:54-59.
25. Wirgart, B. Z., P. Andersson, and L. Grillner. 2005. Evaluation of the ReSSQ assay in relation to the COBAS AMPLICOR CMV MONITOR test and an in-house nested PCR method for detection of cytomegalovirus DNA. J. Clin. Microbiol. 43:4057-4063.
26. Yerly, S., et al. 2007. Cytomegalovirus quantification in plasma by an automated real-time PCR assay. J. Clin. Virol. 38:298-303.