Comparison of QIAsymphony automated and QIAamp manual DNA extraction systems for measuring epstein-barr virus DNA load in whole blood using real-time PCR

Journal of Molecular Diagnostics

Volumn 13, Issue 6, 2011, Pages 695-700

  Laus, Stella ^a   Kingsley, Lawrence A ^b   Green, Michael ^a,c   Wadowsky, Robert M ^a,b,c  

^a CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

^b UNIVERSITY OF PITTSBURGH GRADUATE SCHOOL OF PUBLIC HEALTH   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ARTICLE; AUTOMATION; BLOOD ANALYSIS; COMPARATIVE STUDY; CORRELATION ANALYSIS; DNA EXTRACTION; EPSTEIN BARR VIRUS; HUMAN; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; VIRUS LOAD;

HUMAN HERPESVIRUS 4;

EID: 82955167831     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2011.07.006     Document Type: Article

References (18)

1. Gonzalez I, Green M: Infections of the pediatric liver, intestine, and multivisceral transplant recipient. The Pediatric Transplant Manual, ed 2. Edited by L Remaley, B McGhee, J Reyes, G Mazariegos. Hudson, OH, Lexi-Comp, 2009, pp. 111-129
2. Lee TC, Savoldo B, Rooney CM, Heslop HE, Gee AP, Caldwell Y, Barshes NR, Scott JD, Bristow LJ, O'Mahony CA, Goss JA: Quantitative EBV viral loads and immunosuppression alterations can decrease PTLD incidence in pediatric liver transplant recipients. Am J Transplant 2005, 5:2222-2228 (Pubitemid 41648469)
3. Gulley ML, Tang W: Using Epstein-Barr viral load assays to diagnose, monitor, and prevent posttransplant lymphoproliferative disorder. Clin Microbiol Rev 2010, 23:350-366
4. Stevens SJ, Pronk I, Middeldorp JM: Toward standardization of Epstein-Barr Virus DNA load monitoring: unfractionated whole blood as preferred clinical specimen. J Clin Microbiol 2001, 39:1211-1216 (Pubitemid 32275408)
5. Wadowsky RM, Laus S, Green M, Webber SA, Rowe D: Measurement of Epstein-Barr Virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR. J Clin Microbiol 2003, 41:5245-5249 (Pubitemid 37429951)
6. Fafi-Kremer S, Brengel-Pesce K, Barguès G, Bourgeat MJ, Genoulaz O, Seigneurin JM, Morand P: Assessment of automated DNA extraction coupled with real-time PCR for measuring Epstein-Barr virus load in whole blood, peripheral mononuclear cells and plasma. J Clin Virol 2004, 30:157-164 (Pubitemid 38582023)
7. Mengelle C, Legrand-Abravanel F, Mansuy JM, Barthe C, Da Silva I, Izopet J: Comparison of two highly automated DNA extraction systems for quantifying Epstein-Barr virus in whole blood. J Clin Virol 2008, 43:272-276
8. Raggam RB, Wagner J, Bozic M, Michelin BD, Hammerschmidt S, Homberg C, Kessler H: Detection and quantification of Epstein-Barr virus (EBV) DNA in EDTA whole blood samples using automated sample preparation and real time PCR. Clin Chem Lab Med 2010, 48:413-418
9. Pillet S, Bourlet T, Pozzetto B: Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of Epstein-Barr virus and cytomegalovirus load. J Clin Microbiol 2009, 47:3753-3755
10. Schuurman T, van Breda A, de Boer R, Kooistra-Smid M, Beld M, Savelkoul P, Boom R: Reduced PCR sensitivity due to impaired DNA recovery with the MagNA Pure LC total nucleic acid isolation kit. J Clin Microbiol 2005, 43:4616-4622 (Pubitemid 41298252)
11. Kimura H, Morita M, Yabuta Y, Kuzushima K, Kato K, Kojima S, Matsuyama T, Morishima T: Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J Clin Microbiol 1999, 37:132-136 (Pubitemid 29004193)
12. Lai KK, Cook L, Krantz E, Corey L, Jerome KR: Calibration curves for real-time PCR. Clin Chem 2005, 51:1132-1136 (Pubitemid 43079389)
13. Lo YM, Chan LY, Lo KW, Leung SF, Zhang J, Chan AT, Lee JC, Hjelm NM, Johnson PJ, Huang DP: Quantitative analysis of cell-free Epstein-Barr Virus DNA in plasma of patients with nasopharyngeal carcinoma. Cancer Res 1999, 59:1188-1191 (Pubitemid 29136244)
14. Michelin BD, Hadzisejdic I, Bozic M, Grahovac M, Hess M, Grahovac B, Marth B, Kessler HK: Detection of cytomegalovirus (CMV) DNA in EDTA whole-blood samples: evaluation of the quantitative Artus CMV LightCycler PCR kit in conjunction with automated sample preparation. J Clin Microbiol 2008, 46:1241-1245 (Pubitemid 351563562)
15. Bruce AW: Basic Quality Assurance and Quality Control in the Clinical Laboratory. Boston, Little, Brown, 1984
16. Steel RGD, Torrie JH: Principles and Procedures of Statistics, with Special Reference to the Biological Sciences, ed 1. New York, McGraw-Hill, 1960
17. Hayden RT, Hokanson KM, Pounds SB, Bankowski MJ, Belzer SW, Carr J, Diorio D, Forman MS, Joshi Y, Hillyard D, Hodinka RL, Nikiforova MN, Romain CA, Stevenson J, Valsamakis A, Balfour HH Jr; U.S. EBV Working Group: multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus. J Clin Microbiol 2008, 46:157-163
18. Preiksaitis JK, Pang XL, Fox JD, Fenton JM, Caliendo AM, Miller GG; American Society of Transplantation Infectious Diseases Community of Practice: Interlaboratory comparison of Epstein-Barr virus load assays. Am J Transplant 2009, 9:269-279