Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities

BMC Complementary and Alternative Medicine

Volumn 12, Issue , 2012, Pages

  Alam, Nadia ^a   Hossain, Monzur ^a   Mottalib, Md A ^b   Sulaiman, Siti A ^c   Gan, Siew H ^c   Khalil, Md I ^c,d  

^a UNIVERSITY OF RAJSHAHI   (Bangladesh)

^b Bangladesh Institute of Research and Rehabilitation in Diabetes Endocrine and Metabolic Disorders   (Bangladesh)

^c SCHOOL OF MEDICAL SCIENCES   (Malaysia)

^d JAHANGIRNAGAR UNIVERSITY   (Bangladesh)

Author keywords

Antibacterial; Antioxidant; FRAP; Free radicals; Withania somnifera

Indexed keywords

1,1 DIPHENYL 2 PICRYLHYDRAZYL; ANTHOCYANIN; ASCORBIC ACID; BETA CAROTENE; BUTYLCRESOL; WITHANIA SOMNIFERA EXTRACT;

ANTIBACTERIAL ACTIVITY; ANTIOXIDANT ACTIVITY; ARTICLE; BLEACHING; CITROBACTER FREUNDII; CONTROLLED STUDY; ESCHERICHIA COLI; FRUIT; GRAM NEGATIVE BACTERIUM; IRON CHELATION; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PLANT LEAF; PLANT ROOT; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHI; WITHANIA SOMNIFERA;

EID: 84866996642     PISSN: None     EISSN: 14726882     Source Type: Journal    
DOI: 10.1186/1472-6882-12-175     Document Type: Article

References (41)

1. Ostrowska B, Rzemy KZ. Antioxidant effects a basis of drug selection. Herba Pol 1998, 44(4):417.
2. Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Compl Alternative Med 2008, 8:63.
3. Yazdanparast R, Bahramikias S, Ardestani A. Nasturtium oficinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact 2008, 172:176-184.
4. Jovanovic SV, Simic MG. Antioxidants in nutrition. Ann NY AcadSci 2000, 899:326-334.
5. Sangwan RS, Chaurasiya ND, Misra LN, Lal P, Uniyal GC, Sharma R, Sangwan NS, Suri KA, Qazi GN, Tuli R. Phytochemical variability in commericial herbal products and preparations of Withania somnifera (ashwagandha). Curr Sci 2004, 86:461-465.
6. Kulkarni SK, George B, Mathur R. Protective effect of Withania somnifera root extract on electrographic activity in a lithiumpilocarpine model of status epilepticus. Phytother Res 1998, 12:451-453.
7. Facciola S. Cornucopia - A Source Book of Edible Plants 1990, Vista: Kampong Publications.
8. Alam N, Hossain M, Khalil MI, Moniruzzaman M, Sulaiman SA, Gan SH. High catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis. BMC Compl Alternative Med 2011, 11:65.
9. Jain P, Varshney R. Antimicrobial activity of aqueous and methanolic extracts of Withania somnifera (Ashwagandha). J Chem Pharmaceut Res 2011, 3(3):260-263.
10. Acharyya S, Patra A, Bag PK. Evaluation of the antimicrobial activity of some medicinal plants against enteric bacteria with particular reference to multi-drug resistant Vibrio cholerae. Trop J Pharmaceut Res 2009, 8(3):231-237.
11. Pietta P. Flavonoides as antioxidant. J Nat Prod 2000, 63:1035-1042.
12. Kahkonen MP, Hopia AI, Vuorela HJPRJ, Pihlaja K, Kujala TS, Heinonen M. Antioxidant Activity of Plant Extracts Containing Phenolic Compounds. J Agric Food Chem 1999, 47:3954-3962.
13. Khalil MI, Moniruzzaman M, Boukraâ L, Benhanifia M, Islam MA, Islam MN, Sulaiman SA, Gan SH. Physicochemical and Antioxidant Properties of Algerian Honey. Molecules 2012, 17:11199-11215.
14. Gulcin I. Antioxidant properties of resveratrol: a structure-activity insight. 2010, 11:210-218.
15. Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem 2009, 114:1438-1443.
16. Hatano T, Kagawa H, Yashura T, Okuda T. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chem Pharmaceut Bull 1988, 36:2090-2097.
17. Benzie IFF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999, 299:15-27.
18. Haro-Vicente JF, Martinez-Gracia C, Ros G. Optimization of in vitro measurement of available iron from different fortificants in citric fruit juices. Food Chem 2006, 98:639-648.
19. Gulcin I. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology 2006, 217:213-220.
20. Gülçin I, Elias R, Gepdiremen A, Chea A, Topal F. Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline. J Enzym Inhib Med Chem 2010, 25:44-53.
21. Mi Y, Tae Hun K, Nak Ju S. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 2003, 82:593-597.
22. Ronald M, . Atlas Micro-organisms in our World 1995, 765 blz. St. Louis, Missouri: Mosby-Year Book, Inc, Atlas.
23. Cheesbrough M. District laboratory practice in tropical countries. ECBS Cambridge University Press edition 2000, 2:256-267.
24. Perez C, Pauli M, Bazerque P. An antibiotic assay by agar well diffusion method. Acta Biologica Medica Exp 1990, 15:113-115.
25. Ibekwe VI, Nnanyere NF, Akujobi CO. Studies on Antibacterial activity and phytochemical qualities of various plant extracts. Int J Environ, Health and Human Development 2001, 2(1):41-46.
26. Koleva II, Van Beek TA, Linssen JPH, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002, 13:8-17.
27. Kaileh M, Vanden Berghe W, Heyerick A, Horion J, Piette J, Libert C, De Keukeleire D, Essawi T, Haegeman G. Withaferin A strongly elicits IkappaB kinase beta hyper- phosphorylation concomitant with potent inhibition of its kinase activity. J Biol Chem 2007, 282:4253-4264.
28. Hussain I, Khan L, Marwat GA. Analysis of Vitamin C in Selected Medicinal Plants. J Chem Soc Pakistan 2011, 33(2):260-262.
29. Jaleel CA. Antioxidant profile changes in leaf and root tissues of Withania somnifera Dunal Plant. Omics Journal 2009, 2(4):163-168.
30. Nielsen ILF, Haren GR, Magnussen EL, Dragsted LO, Rasmussen SE. Quantification of anthocyanins in commercial black currant juices by simple high-performance liquid chromatography. Investigation of their pH stability and antioxidative potency. J Agric Food Chem 2003, 51:5861-5866.
31. Frankel E. Nutritional benefits of flavonoids. International conference on food factors: Chemistry and Cancer Prevention 1995, Hamamatsu: Japan Abstracts, C6- 2.
32. Kessler M, Ubeaud G, Jung L. Anti- and pro-oxidant activity of rutin and quercetin derivatives. J Pharm Pharmacol 2003, 55:131-142.
33. Seeram, Navindra PS. Berry Fruits: Compositional Elements, Biochemical Activities, and the Impact of Their Intake on Human Health, Performance, and Disease. J Agric Food Chem 2008, 56(3):627-629.
34. Butelli E, Titta L, Giorgio M, Mock HP, Matros A, Peterek S, Schijlen EGWM, Hall RD, Bovy AG, Luo J, et al. Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 2008, 26(11):1301-1308.
35. Sharma RK, Samant SS, Sharma P, Devi S. Evaluation of antioxidant activities of Withania somnifera leaves growing in natural habitats of North-west Himalaya, India. Journal of Medicinal Plants Research 2012, 6(5):657-661.
36. Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng Jyur (Chrysenthemum morifolium Ramat). Lebnes wiss Technol 1999, 32:269-277.
37. Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf Ex DC), Sage (Savia triloba L.), and Black Tea (Camellia sinensis) extracts. J Agric Food Chem 2000, 48(10):5030-5034.
38. Ramarathnam N, Ochi H, Takeuchi M. Antioxidant defense system in vegetable extracts. Natural Antioxidants; Chemistry, Health Effects and Application 1997 1997, 76-87. Champaign, IL: AOAC Press, Shahidi F.
39. Vattem D, Lin YT, Labbe RG, Shetty K. Antimicrobial activity against select food-borne pathogens by phenolic antioxidants enriched in cranberry pomace by solid-state bioprocessing using food grade fungus Rhizopus oligosporus. Process Biochem 2004, 39:1939-1946.
40. Haslam E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J Nat Prod 1996, 59:205-215.
41. Scalbert A. Antimicrobial properties of tannins. Phytochemistry 1991, 30(12):3875-3883.