A fast and sensitive quantitative lateral flow immunoassay for cry1AB based on a novel signal amplification conjugate

Sensors (Switzerland)

Volumn 12, Issue 9, 2012, Pages 11684-11696

  Chen, Chunxiang ^a   Wu, Jian ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

Cry1Ab; Fluorescence dye; Lateral flow biosensor; Polylysine

Indexed keywords

BIOTIN-STREPTAVIDIN; CRY1AB; LATERAL FLOW; LATERAL FLOW IMMUNOASSAY; LATERAL FLOW IMMUNOASSAYS (LFIA); POLYLYSINE; REACTION CONDITIONS; SIGNAL AMPLIFICATIONS;

BIOSENSORS; FLUORESCENCE; PROTEINS; SIGNAL PROCESSING;

BIOASSAY;

ANTIBODY; BIOTIN; FLUORESCENT DYE; POLYLYSINE;

ARTICLE; CHEMISTRY; CRY1AB; IMMUNOASSAY; LATERAL FLOW BIOSENSOR; LIMIT OF DETECTION; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

CRY1AB; FLUORESCENCE DYE; LATERAL FLOW BIOSENSOR; POLYLYSINE;

ANTIBODIES; BIOTIN; FLUORESCENT DYES; IMMUNOASSAY; LIMIT OF DETECTION; POLYLYSINE; SENSITIVITY AND SPECIFICITY;

EID: 84866996284     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s120911684     Document Type: Article

References (46)

1. Paula, V.; Steinkea, K.; Meyer, H. Development and validation of a sensitive enzyme immunoassay for surveillance of Cry1Ab toxin in bovine blood plasma of cows fed Bt-maize (MON810). Anal. Chim. Acta 2008, 607, 106-113.
2. Wang, S.; Guo, A.Y.; Zheng, W.J.; Zhang, Y.; Qiao, H.; Kennedy, I.R. Development of ELISA for the determination of transgenic Bt-Cottons using antibodies against Cry1Ac protein from Bacillus thuringiensis HD-73. Eng. Life. Sci. 2007, 7, 149-154.
3. Nakajima, O.; Teshima, R.; Takagi, K.; Okunuki, H.; Sawada, J. ELISA method for monitoring human serum IgE specific for Cry1Ab introduced into genetically modified corn. Regul. Toxicol. Pharm. 2007, 47, 90-95.
4. Walschus, U.; Witt, S.; Wittmann, C. Development of monoclonal antibodies against Cry1Ab protein from Bacillus thuringiensis and their application in an ELISA for detection of transgenic Bt-Maize. Food Agr. Immunol. 2002, 14, 231-240.
5. Kumar, R.; Singh, C.K.; Kamle, S.; Sinha, R.P.; Bhatnagar, R.K.; Kachru, D.N. Development of nanocolloidal gold based immunochromatographic assay forrapid detection of transgenic vegetative insecticidal protein in geneticallymodified crops. Food Chem. 2010, 122, 1298-1303.
6. Volpe, G.; Ammid, N.H.; Moscone, D.; Occhigrossi, L.; Palleschi, G. Development of an immunomagnetic electrochemical sensor for detection of BT-CRY1AB/CRY1AC proteins in genetically modified corn samples. Anal. Lett. 2006, 39, 1599-1609.
7. Giovannoli, C.; Anfossi, L.; Baggiani, C.; Giraudi, G. Binding properties of a monoclonal antibody against the Cry1Ab from Bacillus Thuringensis for the development of a capillary electrophoresis competitive immunoassay. Anal. Bioanal. Chem. 2008, 392, 385-393.
8. Jang, H.J.; Cho, I.H.; Kim, H.S.; Jeon, J.W.; Hwang, S.Y.; Paek, S.H. Development of a chemiluminometric immunosensor array for on-site monitoring of genetically modified organisms. Sens. Actuator B 2011, 155, 598-605.
9. Székács, A.; Lauber, É.; Takács, E.; Darvas, B. Detection of Cry1Ab toxin in the leaves of MON 810 transgenic maize. Anal. Bioanal. Chem. 2010, 396, 2203-2211.
10. Roda, A.; Mirasoli, M.; Guardigli, M.; Michelini, E.; Simoni, P.; Magliulo, M. Development and validation of a sensitive and fast chemiluminescent enzyme immunoassay for the detectionof genetically modified maize. Anal. Bioanal. Chem. 2006, 384, 1269-1275.
11. Ermolli, M.; Prospero, A.; Balla, B.; Querci, M.; Mazzeo, A.; Eede, G.V.D. Development of an innovative immunoassay for CP4EPSPS and Cry1AB genetically modified protein detection and quantification. Food Addit. Contam. 2006, 23, 876-882.
12. Fantozzi, A.; Ermolli, M.; Marini, M.; Scotti, D.; Balla, B.; Querci, M.; Langrell, S.R.H.; Eede, G.V.D. First application of a microsphere-based immunoassay to the detection of genetically modified organisms (GMOs): Quantification of Cry1Ab protein in genetically modified maize. J. Agric. Food Chem. 2007, 55, 1071-1076.
13. Li, D.Y.; Fan, K.; Wu, J.; Ying, Y.B. Magnetic beads transfer based assay for CrylAb protein. Chin. J. Anal. Chem. 2011, 39, 1318-1322.
14. Zhu, X.L.; Chen, L.L.; Shen, P.; Jia, J.W.; Zhang, D.B.; Yang, L.T. High sensitive detection of Cry1Ab protein using a quantum dot-based fluorescence-linked immunosorbent assay. J. Agric. Food Chem. 2011, 59, 2184-2189.
15. Pyo, D.J. Comparison of fluorescence immunochromatographic assay strip and gold colloidal immunochromatographic assay strip for detection of microcystin. Anal. Lett. 2007, 40, 907-919.
16. Oh, S.W.; Kim, Y.M.; Kim, H.J.; Kim, S.J.; Cho, J.S.; Choi, E.Y. Point-of-care fluorescence immunoassay for prostate specific antigen. Clin. Chem. Acta 2009, 406, 18-22.
17. Yoo, J.S.; Jung, Y.M.; Hahn, J.H.; Pyo, D.J. Quantitative analysis of a prostate-specific antigen in serum using fluorescence immunochromatography. J. Immunoassay Immunochem. 2010, 31, 259-265.
18. Zaytseva, N.V.; Montagna, R.A.; Lee, E.M.; Baeumner, A.J. Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus. Anal. Bioanal. Chem. 2004, 380, 46-53.
19. Edwards, K.A.; Baeumner, A.J. Liposomes in analyses. Talanta 2006, 68, 1421-1431.
20. Zhan, W.; Bard, A.J. Electrogenerated chemiluminescence: Immunoassay of human C-reactive protein by using Ru(bpy)3 2+-encapsulated liposomes as labels. Anal. Chem. 2007, 79, 459-463.
21. Khreich, N.; Lamourette, P.; Lagoutte, B.; Ronco, C.; Franck, X.; Créminon, C.; Volland, H. A fluorescent immunochromatographic test using immunoliposomes for detecting microcystins and nodularins. Anal. Bioanal. Chem. 2010, 397, 1733-1742.
22. Khreich, N.; Lamourette, P.; Boutal, H.; Devilliers, K.; Créminon, C.; Volland, H. Detection of Staphylococcus enterotoxin B using fluorescent immunoliposomes as label for immunochromatographic testing. Anal. Biochem. 2008, 377, 182-188.
23. Zou, Z.X.; Du, D.; Wang, J.; Smith, J.N.; Timchalk, C.; Li, Y.Q.; Lin, Y.H. Quantum dot-based immunochromatographic fluorescent biosensor for biomonitoring trichloropyridinol, a biomarker of exposure tochlorpyrifos. Anal. Chem. 2010, 82, 5125-5133.
24. Chen, Y.P.; Ning, B.A.; Liu, N.; Feng, Y.; Liu, Z.; Liu, X.Y.; Gao, Z.X. A rapid and sensitive fluoroimmunoassay based on quantum dot for the detection of chlorpyrifos residue in drinking water. J. Environ. Sci. Heal. B 2010, 45, 508-515.
25. Chen, J.X.; Xu, F.; Jiang, H.Y.; Hou, Y.L.; Rao, Q.X.; Guo, P.J.; Ding, S.Y. A novel quantum dot-based fluoroimmunoassay method for detection of Enrofloxacin residue in chicken muscle tissue. Food Chem.2009, 113, 1197-1201.
26. Li, Z.H.; Wang, Y.; Wang, J.; Tang, Z.W.; Pounds, J.G.; Lin, Y.H. Rapid and sensitive detection of protein biomarker using a portable fluorescence biosensor based on quantum dots and a lateral flow test strip. Anal. Chem. 2010, 82, 7008-7014.
27. Yang, H.; Li, D.; He, D.; Guo, Q.; Wang, K.; Zhang, X.Q.; Huang, P.; Cui, D.X. A noval quantum dots-based point of care test for syphilis. Nanoscale Res. Lett. 2010, 5, 875-881.
28. Mateo, C.; Palomo, J.M.; Langen, L.M.V.; Rantwijk, F.V.; Sheldon, R.A. A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates. Biotechnol. Bioeng. 2004, 86, 273-276.
29. Fuentes, M.; Segura, R.L.; Abian, O.; Betancor, L.; Hidalgo, A.; Mateo, C.; Fernandez-Lafuente, R.; Guisan, J.M. Determination of protein-protein interactions through aldehyde-dextran intermolecular cross-linking. Proteomics 2004, 4, 2602-2607.
30. Dhawan, S. Design construction of novel molecular conjugates for signal amplification (I): Conjugation of multiple horseradish peroxidase molecules to immunoglobulin via primary amines on lysine peptide chains. Peptides 2002, 23, 2091-2098.
31. Chang, W.F.; Wang, S.J.; Lai, S.F.; Shieh, C.J.; Hsiung, K.P.; Liu, Y.C. Evaluation on the use of reactive dye-modified polylysine as the biomarker in immunochromatographic test application. Anal. Biochem. 2011, 411, 236-240.
32. He, Y.Q.; Zhang, S.Q.; Zhang, X.B.; Baloda, M.; Gurung, A.S.; Xu, H.; Zhang, X.J.; Liu, G.D. Ultrasensitive nucleic acid biosensor based on enzyme-gold nanoparticle dual label and lateral flow strip biosensor. Biosens. Bioelectron. 2011, 26, 2018-2024.
33. Wu, Y.F.; Chen, C.L.; Liu, S.Q. Enzyme-functionalized silica nanoparticles as sensitive labels in biosensing. Anal. Chem. 2009, 81, 1600-1607.
34. Piao, Y.; Burns, A.; Kim, J.; Wiesner, U.; Hyeon, T. Designed fabrication of silica-based nanostructured particle systems for nanomedicine applications. Adv. Funct. Mater. 2008, 18, 3745-3758.
35. Chen, L.; Chen, C.; Li, R.; Li, Y.; Liu, S. CdTe quantum dot functionalized silica nanosphere labels for ultrasensitive detection of biomarker. Chem. Commun. 2009, 19, 2670-2672.
36. Wang, J.; Liu, G.; Engelhard, M.H.; Lin, Y. Sensitive immunoassay of a biomarker tumor necrosis factor-α based on poly(guanine)-functionalized silica nanoparticle label. Anal. Chem. 2006, 78, 6974-6979.
37. Ke, R.Q.; Yang, W.; Xia, X.H.; Xu, Y.; Li, Q.G. Tandem conjugation of enzyme and antibody on silica nanoparticle for enzyme immunoassay. Anal. Biochem. 2010, 406, 8-13.
38. Xia, X.H.; Xu, Y.; Zhao, X.L.; Li, Q.G. Lateral flow immunoassay using europium chelate-loaded silica nanoparticles as labels. Chin. Chem. 2009, 55, 179-182.
39. Wang, Y.Y.; Xu, H.; Wei, M.; Gu, H.C.; Xu, Q.F.; Zhu, W. Study of superparamagnetic nanoparticles as labels in the quantitative lateral flow immunoassay. Mater. Sci. Eng. 2009, 29, 714-718.
40. Zheng, C.; Wang, X.C.; Lu, Y.; Liu, Y. Rapid detection of fish major allergen parvalbumin using superparamagnetic nanoparticle-based lateral flow immunoassay. Food Control. 2012, 26, 446-452.
41. Tang, D.; Sauceda, J.C.; Lin, Z.; Ott, S.; Basova, E.; Goryacheva, I.; Biselli, S.; Lin, J.; Niessner, R.; Knopp, D. Magnetic nanogold microspheres-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food. Biosens. Bioelectron. 2009, 25, 514-518.
42. Yan, J.L.; Estevez, M.C.; Smith, J.E.; Wang, K.M.; He, X.X.; Wang, L.; Tan, W.H. Dye-doped nanoparticles for bioanalysis. Nano Today 2007, 2, 44-50.
43. Valanne, A.; Suojanen, J.; Peltonen, J.; Soukka, T. Hanninen, P.; Harma, H. Multiplesized europium(III) chelate-dyed polystyrene particles as donors in FRET: An application for sensitive protein quantification utilizing competitive adsorption. Analyst 2009, 134, 980-986.
44. Juntunen, E.; Myyryläinen, T.; Salminen, T.; Soukka, T.; Pettersson, K. Performance of fluorescent europium (III) nanoparticles and colloidal gold reporters in lateral flow bioaffinity assay. Anal. Biochem. 2012. 428, 31-38.
45. Liu, R.P.; Liu, J.T.; Xie, L.; Wang, M.X.; Luo, J.P.; Cai, X.X. A fast and sensitive enzyme immunoassay for brain natriuretic peptide based on micro-magnetic probes strategy. Talanta 2010, 81, 1016-1021.
46. Sai, N.; Chen, Y.P.; Liu, N.; Yu, G.G.; Su, P.; Feng, Y.; Zhou, Z.J.; Liu, X.Y.; Zhou, H.Y.; Gao, Z.X.; Ning, B.A. A sensitive immunoassay based on direct hapten coated format and biotin-streptavidin system for the detection of chloramphenicol. Talanta 2010, 82, 1113-1121.