Development of an immunomagnetic electrochemical sensor for detection of Bt-Cry1Ab/Cry1Ac proteins in genetically modified corn samples

Analytical Letters

Volumn 39, Issue 8, 2006, Pages 1599-1609

  Volpe, G ^a   Ammid, N H ^b   Moscone, D ^a   Occhigrossi, L ^a   Palleschi, G ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b UNIVERSITY OF BENGHAZI   (Libyan Arab Jamahiriya)

Author keywords

GMO; Immunomagnetic beads; Screen printed electrodes

Indexed keywords

ZEA MAYS;

EID: 33745062167     PISSN: 00032719     EISSN: 1532236X     Source Type: Journal    
DOI: 10.1080/00032710600713339     Document Type: Conference Paper

References (14)

1. Brett, G.M., Chambers, S.J., Huang, L., and Morgan, M.R.A. 1999. Design and development of immunoassays for detection of proteins. Food Control, 10: 401-406.
2. Clark, B.W., Phillips, T.A., and Coats, J.R. 2005. Environmental fate and effects of Bacillus thurigiensis (Bt) proteins from transgenic crops: a review. J. Agric. Food Chem., 53: 4643-4653.
3. Fagan, J. et al. 2001. Performance assessment under field conditions of a rapid immunological test for transgenic soybeans. Int. J. Food Sci. Technol., 36: 1-11.
4. Federici, B. 1998. Broadscale use of pest-killing plants to be true test. Calif. Agric, 52: 14-20.
5. Head, G., Surber, J.B., Watson, J.A., Martin, J.W., and Duan, J.J. 2002. No detection of Cry 1Ac protein in soil after multiple years of transgenic Bt cotton (Bollgard) use. Environ. Entomol., 31 (1): 30-36.
6. Hubner, P., Studer, E., and Luthy, J. 1999. Quantitative competitive PCR for the detection of genetically modified organisms in food. Food Control, 10: 353-358.
7. Lewellyn, D., Cousins, Y., Mathews, A., Hartweek, L., and Lyon, B. 1994. Expression of Bacillus thurigiensis insecticidal protein genes in transgenic crop plants. Agric. Ecosys. Environ., 49: 85-93.
8. Lipp, M. et al. 2000. Validation of an immunoassay for the detection and quantification of Roudup-Ready soybeans in food and food fractions by the use of reference material. J. AOAC Int., 83: 99-127.
9. Meyer, R. 1999. Development and application of DNA analytical methods for the detection of GMOs in food. Food Control, 10: 391-399.
10. Pasqualone, A. 2000. Metodi di analisi per Ia rivelazione di OGM negli alimenti. Industrie Alimentari, XXXIX (aprile): 444-451.
11. Stave, J.M. 1999. Detection of new or modified proteins in novel food derived from GMO-future needs. Food Control, 10: 367-374.
12. Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., De Beuckeleer, J., Dean, C., Zabeau, M., Van Montagu, M., and Leemans, J. 1987. Transgenic plants protected from insect attack. Nature, 328: 33-37.
13. Vaitilingom, M. et al. 1999. Real-time quantitative PCR detection of genetically modified maximizer maize and Roundup Ready Soybean in some representative foods. J. Agric. Food Chem., 47: 5261-5266.
14. Walschus, U., Witt, S., and Wittmann, C. 2002. Development of monoclonal antibody against Cry 1Ab protein from Bacillus thurigiensis and their application in an ELISA for detection of transgenic Bt-Maize. Food Agric. Immunol., 14 (2): 231-240.