An enhanced approach for engineering thermally stable proteins using yeast display

Protein Engineering, Design and Selection

Volumn 25, Issue 10, 2012, Pages 625-630

  Pavoor, Tej V ^a   Wheasler, Jean A ^a   Kamat, Viraj ^a   Shusta, Eric V ^a  

^a University of Wisconsin Madison   (United States)

Author keywords

protein engineering; thermal stability; yeast surface display

Indexed keywords

BIOTECHNOLOGY APPLICATIONS; FITNESS LANDSCAPE; GREEN FLUORESCENT PROTEIN; PROTEIN ENGINEERING; PROTEIN STABILITY; SURFACE DISPLAYS; THERMAL DENATURATIONS; THERMALLY STABLE; VALIDATED METHODS; YEAST DISPLAY;

GENETIC ENGINEERING; THERMODYNAMIC STABILITY; YEAST;

PROTEINS;

ENHANCED GREEN FLUORESCENT PROTEIN; FUNGAL PROTEIN;

ARTICLE; CLONE; ENRICHMENT CULTURE; FUNGAL VIABILITY; HEAT TOLERANCE; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN STABILITY; THERMOSTABILITY; YEAST; YEAST DISPLAY;

AMINO ACID SEQUENCE; GREEN FLUORESCENT PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

EID: 84866985849     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs041     Document Type: Article

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