Reversible cycling between cysteine persulfide-ligated [2Fe-2S] and cysteine-ligated [4Fe-4S] clusters in the FNR regulatory protein

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 39, 2012, Pages 15734-15739

  Zhang, Bo ^a   Crack, Jason C ^b   Subramanian, Sowmya ^a   Green, Jeffrey ^c   Thomson, Andrew J ^b   Le Brun, Nick E ^b   Johnson, Michael K ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CYSTEINE; FUMARATE AND NITRATE REDUCTION REGULATORY PROTEIN; OXYGEN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANAEROBIC METABOLISM; ARTICLE; DNA BINDING; ESCHERICHIA COLI; MASS SPECTROMETRY; NONHUMAN; OXYGEN SENSING; PRIORITY JOURNAL; RAMAN SPECTROMETRY; ULTRAVIOLET SPECTROPHOTOMETRY;

CYSTEINE; ESCHERICHIA COLI PROTEINS; FERROUS COMPOUNDS; IRON-SULFUR PROTEINS; MODELS, CHEMICAL; OXYGEN; SPECTRUM ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84866876886     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1208787109     Document Type: Article

References (44)

1. Spiro S, Guest JR (1990) FNR and its role in oxygen-regulated gene expression in Escherichia coli. FEMS Microbiol Rev 6:399-428. (Pubitemid 20281613)
2. Guest JR, Green J, Irvine AS, Spiro S (1996) in Regulation of Gene Expression in Escherichia coli, eds Lin ECC, Lynch AS (R. G. Landes, Austin, TX), pp 317-342.
3. Constantinidou C, et al. (2006) A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth. J Biol Chem 281:4802-4815. (Pubitemid 43847742)
4. Crack JC, et al. (2008) Signal perception by FNR: The role of the iron-sulfur cluster. Biochem Soc Trans 36:1144-1148.
5. Green J, Crack JC, Thomson AJ, Le Brun NE (2009) Bacterial sensors of oxygen. Curr Opin Microbiol 12:145-151.
6. Kiley PJ, Beinert H (2003) The role of Fe-S proteins in sensing and regulation in bacteria. Curr Opin Microbiol 6:181-185. (Pubitemid 36628663)
7. Fleischhacker AS, Kiley PJ (2011) Iron-containing transcription factors and their roles as sensors. Curr Opin Chem Biol 15:335-341.
8. Shaw DJ, Rice DW, Guest JR (1983) Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli. J Mol Biol 166:241-247.
9. Green J, Sharrocks AD, Green B, Geisow M, Guest JR (1993) Properties of FNR proteins substituted at each of the five cysteine residues. Mol Microbiol 8:61-68. (Pubitemid 23114822)
10. 2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity. Proc Natl Acad Sci USA 94:6087-6092.
11. Kiley PJ, Beinert H (1998) Oxygen sensing by the global regulator, FNR: The role of the iron-sulfur cluster. FEMS Microbiol Rev 22:341-352. (Pubitemid 29074341)
12. Popescu CV, Bates DM, Beinert H, Münck E, Kiley PJ (1998) Mössbauer spectroscopy as a tool for the study of activation/inactivation of the transcription regulator FNR in whole cells of Escherichia coli . Proc Natl Acad Sci USA 95:13431-13435.
13. Lazazzera BA, Bates DM, Kiley PJ (1993) The activity of the Escherichia coli transcription factor FNR is regulated by a change in oligomeric state. Genes Dev 7:1993-2005. (Pubitemid 23312951)
14. Lazazzera BA, Beinert H, Khoroshilova N, Kennedy MC, Kiley PJ (1996) DNA binding and dimerization of the Fe-S-containing FNR protein from Escherichia coli are regulated by oxygen. J Biol Chem 271:2762-2768. (Pubitemid 26047894)
15. 2+ cluster of FNR from Escherichia coli. Biochemistry 43:791-798.
16. (2) on the Fe-S cluster biogenesis requirements of Escherichia coli FNR. J Mol Biol 384:798-811.
17. Crack JC, Green J, Thomson AJ (2004) Mechanism of oxygen sensing by the bacterial transcription factor fumarate-nitrate reduction (FNR). J Biol Chem 279:9278- 9286.
18. Crack JC, Green J, Le Brun NE, Thomson AJ (2006) Detection of sul fide release from the oxygen-sensing [4Fe-4S] cluster of FNR. J Biol Chem 281:18909-18913. (Pubitemid 44035392)
19. Crack JC, Green J, Cheesman MR, Le Brun NE, Thomson AJ (2007) Superoxide-mediated amplification of the oxygen-induced switch from [4Fe-4S] to [2Fe-2S] clusters in the transcriptional regulator FNR. Proc Natl Acad Sci USA 104:2092-2097. (Pubitemid 46391357)
20. 2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion. Proc Natl Acad Sci USA 106:4659-4664.
21. 2+ Cluster. J Bacteriol 186:8018-8025.
22. 4 clusters: Resonance Raman evidence for different degrees of distortion in HiPIP and ferredoxin. J Am Chem Soc 109:7178-7187.
23. 2H substitution: Coupling of Fe-S stretching and S-C-C bending modes. J Am Chem Soc 111:3496-3504.
24. 2 protein resonance Raman revisited: Structural variations among adrenodoxin, ferredoxin, and red paramagnetic protein. J Am Chem Soc 111:3505-3511.
25. Fu W, Drozdzewski PM, Davies MD, Sligar SG, Johnson MK (1992) Resonance Raman and magnetic circular dichroism studies of reduced [2Fe-2S] proteins. J Biol Chem 267: 15502-15510.
26. 2+cluster switch in the transcriptional regulator FNR. J Am Chem Soc 130:1749-1758.
27. Johnson MK, Czernuszewicz RS, Spiro TG, Fee JA, Sweeney WV (1983) Resonance Raman spectroscopic evidence for a common [3Fe-4S] structure among proteins containing three-iron clusters. J Am Chem Soc 103:6671-6678.
28. 4] cluster in a mutant form of Pyrococcus furiosus ferredoxin. J Am Chem Soc 120:8525-8526.
29. Smith AD, et al. (2001) Sulfur transfer from IscS to IscU: The first step in iron-sulfur cluster biosynthesis. J Am Chem Soc 123:11103-11104. (Pubitemid 33042042)
30. Broderick JB, et al. (1997) Pyruvate formate-lyase activating enzyme is an iron-sulfur protein. J Am Chem Soc 119:7396-7397. (Pubitemid 27348602)
31. Ollagnier S, et al. (1999) Assembly of 2Fe-2S and 4Fe-4S clusters in the anaerobic ribonucleotide reductase from Escherichia coli. J Am Chem Soc 121:6344-6350.
32. Cosper MM, et al. (2004) Characterization of the cofactor composition of Escherichia coli biotin synthase. Biochemistry 43:2007-2021. (Pubitemid 38233263)
33. Nashef AS, Osugo DT, Feeney RE (2004) Determination of hydrogen sulfide with 5,5′-dithiobis-(2-nitrobenzoicacid),N-ethylmaleimide, and parachloromercuribenzoate. Anal Biochem 79:394-405.
34. 2- of aconitase during cluster interconversion and removal. J Biol Chem 263:8194-8198.
35. Unciuleac M-C, et al. (2007) In vitro activation of apo-aconitase using a [4Fe-4S] cluster-loaded form of the IscU [Fe-S] cluster scaffolding protein. Biochemistry 46: 6812-6821. (Pubitemid 46906411)
36. Chandramouli K, et al. (2007) Formation and properties of [4Fe-4S] clusters on the IscU scaffold protein. Biochemistry 46:6804-6811. (Pubitemid 46906410)
37. Smith AD, et al. (2005) NifS-mediated assembly of [4Fe-4S] clusters in the N- and C-terminal domains of the NifU scaffold protein. Biochemistry 44:12955-12969. (Pubitemid 41377276)
38. Dos Santos PC, et al. (2004) Iron-sulfur cluster assembly: NifU-directed activation of the nitrogenase Fe protein. J Biol Chem 279:19705-19711. (Pubitemid 38623408)
39. Tan G, Lu J, Bitoun JP, Huang H, Ding H (2009) IscA/SufA paralogues are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions. Biochem J 420:463-472.
40. Mühlenhoff U, Richter N, Pines O, Pierik AJ, Lill R (2011) Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins. J Biol Chem 286:41205-41216.
41. Ding H, Clark RJ (2004) Characterization of iron binding in IscA, an ancient iron-sulphur cluster assembly protein. Biochem J 379:433-440. (Pubitemid 38570116)
42. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
43. Beinert H (1983) Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins. Anal Biochem 131:373-378.
44. Saavedra CP, et al. (2004) Biochemical characterization of a thermostable cysteine synthase from Geobacillus stearothermophilus V. Biochemie 86:481-485. (Pubitemid 39092711)