Kinetic analysis of the oxidative conversion of the [4Fe-4S]2+ cluster of FNR to a [2Fe-2S]2+ cluster

Journal of Bacteriology

Volumn 186, Issue 23, 2004, Pages 8018-8025

  Sutton, Victoria R ^a,b   Mettert, Erin L ^b   Beinert, Helmut ^c   Kiley, Patricia J ^b,d  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^c UNIVERSITY OF WISCONSIN   (United States)

^d NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; IRON COMPLEX; OXYGEN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR FNR; UNCLASSIFIED DRUG;

ARTICLE; ESCHERICHIA COLI; NONHUMAN; OXIDATION; OXYGEN CONSUMPTION; PRIORITY JOURNAL; REACTION ANALYSIS; TRANSCRIPTION REGULATION;

DNA; ESCHERICHIA COLI PROTEINS; IRON; IRON-SULFUR PROTEINS; KINETICS; OXYGEN; PROMOTER REGIONS (GENETICS);

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 9244247618     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.23.8018-8025.2004     Document Type: Article

References (35)

1. Agashe, V. R., S. Guha, H. C. Chang, P. Generaux, M. Hayer-Hartl, M. Stemp, C. Georgopoulos, F. U. Hartl, and J. M. Barral. 2004. Function of trigger factor and DnaK in multidomain protein folding: increase in yield at the expense of folding speed. Cell 117:199-209.
2. Bates, D. M. 1999. Role of iron-sulfur cluster conversion in the oxygen-sensing mechanism of the Escherichia coli transcription factor FNR. Ph.D. thesis. University of Wisconsin-Madison, Madison.
3. 2+ cluster to oxygen. J. Biol. Chem. 275:6234-6240.
4. 2 as the regulatory signal for FNR-dependent gene regulation in Escherichia coli. J. Bacteriol. 178:4515-4521.
5. Beinert, H. 1983. Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins. Anal. Biochem. 131:373-378.
6. Bremer, H., and P. P. Dennis. 1987. Modulation of chemical composition and other parameters of the cell by growth rate, p. 1527-1540. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
7. Crack, J., J. Green, and A. J. Thomson. 2004. Mechanism of oxygen sensing by the bacterial transcription factor fumarate-nitrate reduction (FNR). J. Biol. Chem. 279:9278-9286.
8. 2 and iron availability. Arch. Microbiol. 156:463-470.
9. Farewell, A., and F. C. Neidhardt. 1998. Effect of temperature on in vivo protein synthetic capacity in Escherichia coli. J. Bacteriol. 180:4704-4710.
10. Flint, D. H., J. F. Tuminello, and M. H. Emptage. 1993. The inactivation of Fe-S cluster containing hydro-lyases by superoxide. J. Biol. Chem. 268:22369-22376.
11. Fridovich, I. 1978. The biology of oxygen radicals. Science 201:875-880.
12. Green, J., B. Bennett, P. Jordan, E. T. Ralph, A. J. Thomson, and J. R. Guest. 1996. Reconstitution of the [4Fe-4S] cluster in FNR and demonstration of the aerobic-anaerobic transcription switch in vitro. Biochem. J. 316:887-892.
13. Guest, J. R., J. Green, A. S. Irvine, and S. Spiro. 1996. The FNR modulon and FNR-regulated gene expression, p. 317-342. In E. C. C. Lin and A. S. Lynch (ed.), Regulation of gene expression in Escherichia coli. R. G. Landes Company, Austin, Texas.
14. Imlay, J. A., and I. Fridovich. 1991. Assay of metabolic superoxide production in Escherichia coli. J. Biol. Chem. 266:6957-6965.
15. Jordan, P. A., A. J. Thomson, E. T. Ralph, J. R. Guest, and J. Green. 1997. FNR is a direct oxygen sensor having a biphasic response curve. FEBS Lett. 416:349-352.
16. 2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity. Proc. Natl. Acad. Sci. USA 94:6087-6092.
17. Lamberg, K. E., and P. J. Kiley. 2000. FNR-dependent activation of the class II dmsA and narG promoters of Escherichia coli requires FNR-activating regions 1 and 3. Mol. Microbiol. 38:817-827.
18. Lamberg, K. E., C. Luther, K. D. Weber, and P. J. Kiley. 2002. Characterization of activating region 3 from Escherichia coli FNR. J. Mol. Biol. 315:275-283.
19. Lazazzera, B. A., H. Beinert, N. Khoroshilova, M. C. Kennedy, and P. J. Kiley. 1996. DNA binding and dimerization of the Fe-S-containing FNR protein from Escherichia coli are regulated by oxygen. J. Biol. Chem. 271:2762-2768.
20. Meng, W., J. Green, and J. R. Guest. 1997. FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites. Microbiology 143:1521-1532.
21. Moore, L. J., and P. J. Kiley. 2001. Characterization of the dimerization domain in the FNR transcription factor. J. Biol. Chem. 276:45744-45750.
22. Neidhardt, F. C. 1999. Bacterial growth: constant obsession with dN/dt. J. Bacteriol. 181:7405-7408.
23. Patschkowski, T., D. M. Bates, and P. J. Kiley. 2000. Mechanisms for sensing and responding to oxygen deprivation, p. 61-78. In G. Storz and R. Hengge-Aronis (ed.), Bacterial stress responses. ASM Press, Washington, D.C.
24. Popescu, C. V., D. M. Bates, H. Beinert, E. Münck, and P. J. Kiley. 1998. Mössbauer spectroscopy as a tool for the study of activation/inactivation of the transcription regulator FNR in whole cells of Escherichia coli. Proc. Natl. Acad. Sci. USA 95:13431-13435.
25. Salmon, K., S. P. Hung, K. Mekjian, P. Baldi, G. W. Hatfield, and R. P. Gunsalus. 2003. Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR. J. Biol. Chem. 278:29837-29855.
26. Subczynski, W. K., J. S. Hyde, and A. Kusumi. 1989. Oxygen permeability of phosphatidylcholine-cholesterol membranes. Proc. Natl. Acad. Sci. USA 86:4474-4478.
27. Sutton, V. R., and P. J. Kiley. 2003. Techniques for studying the oxygen-sensitive transcription factor FNR from Escherichia coli. Methods Enzymol. 370:300-312.
28. 2+ cluster of FNR from Escherichia coli. Biochemistry 43:791-798.
29. Tseng, C. P., J. Albrecht, and R. P. Gunsalus. 1996. Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coli. J. Bacteriol. 178:1094-1098.
30. Tseng, C. P., A. K. Hansen, P. Cotter, and R. P. Gunsalus. 1994. Effect of cell growth rate on expression of the anaerobic respiratory pathway operons frdABCD, dmsABC, and narGHJI of Escherichia coli. J. Bacteriol. 176:6599-6605.
31. R promoter DNA. Biophys. J. 76:1320-1329.
32. 2 and reducing conditions. J. Mol. Microbiol. Biotechnol. 4:263-268.
33. 2-dependent gene regulation in facultatively anaerobic bacteria. Arch. Microbiol. 164:81-90.
34. Unden, G., and A. Duchene. 1987. On the role of cyclic AMP and the Fnr protein in Escherichia coli growing anaerobically. Arch. Microbiol. 147:195-200.
35. Ziegelhoffer, E. C., and P. J. Kiley. 1995. In vitro analysis of a constitutively active mutant form of the Escherichia coli global transcription factor FNR. J. Mol. Biol. 245:351-361.