In vivo endoscopic optical biopsy with optical coherence tomography

Science

Volumn 276, Issue 5321, 1997, Pages 2037-2039

  Tearney, Guillermo J ^a   Brezinski, Mark E ^b,d   Bouma, Brett E ^a   Boppart, Stephen A ^a   Pitris, Costas ^a   Southern, James F ^c   Fujimoto, James G ^a  

^a USA   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c AURORA SINAI MEDICAL CENTER   (United States)

^d MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ENDOSCOPY; IMAGE DISPLAY; IMAGE PROCESSING; IMAGE RECONSTRUCTION; IMAGING SYSTEM; PRIORITY JOURNAL; TOMOGRAPHY;

ANATOMY, CROSS-SECTIONAL; ANIMALS; BIOPSY; CATHETERIZATION; ENDOSCOPES; EPITHELIUM; ESOPHAGOSCOPES; ESOPHAGUS; FIBER OPTICS; INTERFEROMETRY; LASERS; MUCOUS MEMBRANE; RABBITS; SCATTERING, RADIATION; TOMOGRAPHY; TRACHEA;

EID: 0030760309     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5321.2037     Document Type: Article

References (23)

1. D. Huang et al., Science 254, 1178 (1991).
2. A. F. Fercher, C. K. Hitzenberger, W. Drexler, G. Kamp, H. Sattmann, Am. J. Ophthalmol. 116, 113(1993).
3. E. A. Swanson et al., Opt. Lett. 18, 1864 (1993).
4. M. R. Hee et al., Arch. Ophthalmol. 113, 325 (1995).
5. C. A. Puliafito et al., Ophthalmology 102, 217 (1995).
6. J. G. Fujimoto et al., Nature Med. 1, 970 (1995).
7. J. M. Schmitt, A. Knuttel, M. Yadlowsky, M. A. Eckhaus, Phys. Med. Biol. 39, 1705 (1994).
8. M. E. Brezinski et al., Circulation 93, 1206 (1996).
9. J. M. Schmitt, M. J. Yadlowsky, R. F. Bonner, Dermatology 191, 93 (1995).
10. G. J. Tearney et al., Am. J. Gastroenterol., in press.
11. B. E. Bouma, G. J. Tearney, I. P. Bilinsky, B. Golubovic, J. G. Fujimoto, Opt. Lett. 21, 1839 (1996).
12. A. Seas, V. Petricevic, R. R. Alfano, ibid. 17, 937 (1992).
13. V. M. Gelikonov et al., JETP Lett. 61, 149 (1995).
14. G. J. Tearney et al., Opt. Lett. 21, 1408 (1996).
15. K. F. Kwong, D. Yankelevich, K. C. Chu, J. P. Heritage, A. Dienes, ibid. 18, 558 (1993).
16. J. P. Heritage, A. M. Weiner, R. N. Thurston, ibid. 10, 609 (1985).
17. G. J. Tearney et al., ibid. 21, 543 (1996).
18. R. W. Phillips and R. K. H. Wong, Gastroenterol. Clin. N. Am. 20, 791 (1991).
19. G. J. Tearney et al., J. Urol. 157, 1913 (1997).
20. M. E. Brezinski et al., J. Surg. Res., in press.
21. The axial dimension of each OCT image was 2.3 mm, which was digitized to 248 pixels. Imaging could be performed with either 256 or 512 lateral pixels, corresponding to image acquisition times of 125 or 250 ms, respectively. All images were obtained with 512-pixel lateral resolution at four frames per second to optimize lateral sampling. The two-dimensional image data were displayed with a polar coordinate transformation and inverse gray scale. Data were recorded in both Super VHS and digital format.
22. In vivo imaging was performed on normal New Zealand White rabbits at 12 weeks of age. After the animals were anesthetized, the OCT imaging catheter-endoscope was introduced through a 2.3-mm guiding catheter into the gastrointestinal and respiratory tract. The respiratory tract was accessed directly through the trachea via a midline incision, and the gastrointestinal tract was entered through the oropharynx. Sites imaged included the oropharynx, esophagus, trachea, and bronchi (main, secondary, and tertiary). After imaging, the animals were killed and the imaged regions were excised and immersed in 10% formalin for routine histologie processing. The specimens were blocked in paraffin, cut into 5-μm sections, and stained with hematoxylin and eosin (H&E) for microscopic examination.
23. The contributions of E. A. Swanson (MIT Lincoln Laboratory) are greatly appreciated. We thank J. Gamba and J. Taralli for technical support and C. Kopf for help with preparation of the manuscript. Supported in part by NIH contract NIH-9-RO1-EY11289-10 (J.G.F.), the Medical Free Electron Laser Program, Office of Naval Research contract N00014-94-1-0717 (J.G.F.), the Air Force Office of Scientific Research contract F49620-95-1-0221 (J.G.F.), the Joint Services Electronics Program contract DAAH04-95-1-0038 (J.G.F.), the Whittaker Foundation contract 96-0205 (M.E.B.), and NIH contract NIH-1-R29-HL55686-01A1 (M.E.B.).