Contamination from an affinity column: An encounter with a new villain in the world of membrane-protein crystallization

Acta Crystallographica Section D: Biological Crystallography

Volumn 68, Issue 10, 2012, Pages 1272-1277

  Panwar, Pankaj ^a,b,c,d   Deniaud, Aurélien ^a,b,c,e   Pebay Peyroula, Eva ^a,b,c  

^a CEA GRENOBLE   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c UNIV GRENOBLE ALPES   (France)

^d UNIVERSITY OF ALBERTA   (Canada)

^e EUROPEAN MOLECULAR BIOLOGY LABORATORY   (France)

Author keywords

affinity column purification; desthiobiotin; detergents; membrane protein crystallization; Strep Tactin structure; streptavidin

Indexed keywords

ARABIDOPSIS PROTEIN; BIOTIN; DETHIOBIOTIN; DRUG DERIVATIVE; MEMBRANE PROTEIN; STREPTAVIDIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CRYSTALLIZATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN BINDING; X RAY CRYSTALLOGRAPHY;

ARABIDOPSIS PROTEINS; BIOTIN; CHROMATOGRAPHY, AFFINITY; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; MEMBRANE PROTEINS; PROTEIN BINDING; STREPTAVIDIN;

ARABIDOPSIS THALIANA; ESCHERICHIA COLI;

EID: 84866686230     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S090744491202639X     Document Type: Article

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