Differences between CusA and AcrB crystallisation highlighted by protein flexibility

PLoS ONE

Volumn 4, Issue 7, 2009, Pages

  Deniaud, Aurélien ^a   Goulielmakis, Aurérelie ^a   Covès, Jacques ^a   Pebay Peyroula, Eva ^a  

^a INSTITUT DE BIOLOGIE STRUCTURALE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOPHILE; BACTERIAL PROTEIN; CHYMOTRYPSIN; ELASTASE; HEAVY METAL; PAPAIN; PROTEIN ACRB; PROTEIN CUSA; SUBTILISIN; TRYPSIN; UNCLASSIFIED DRUG; ACRE PROTEIN, E COLI; DIVALENT CATION; ESCHERICHIA COLI PROTEIN; MULTIDRUG RESISTANCE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CRYSTALLIZATION; ESCHERICHIA COLI; NODULATION; NONHUMAN; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; SEQUENCE ALIGNMENT; CHEMISTRY; MOLECULAR GENETICS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CATIONS, DIVALENT; CRYSTALLIZATION; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 67650520154     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006214     Document Type: Article

References (30)

1. Nies DH (2003) Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol Rev 27: 313-339.
2. Piddock LJ (2006) Multidrug-resistance efflux pumps - not just for resistance. Nat Rev Microbiol 4: 629-636.
3. Paulsen IT, Park JH, Choi PS, Saier MH Jr (1997) A family of gram-negative bacterial outer membrane factors that function in the export of proteins, carbohydrates, drugs and heavy metals from gram-negative bacteria. FEMS Microbiol Lett 156: 1-8.
4. Paulsen IT, Brown MH, Skurray RA (1996) Proton-dependent multidrug efflux systems. Microbiol Rev 60: 575-608.
5. Franke S, Grass G, Rensing C, Nies DH (2003) Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J Bacteriol 185: 3804-3812.
6. Murakami S, Nakashima R, Yamashita E, Yamaguchi A (2002) Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419: 587-593.
7. Murakami S, Yamaguchi A (2003) Multidrug-exporting secondary transporters. Curr Opin Struct Biol 13: 443-452.
8. Seeger MA, von Ballmoos C, Verrey F, Pos KM (2009) Crucial role of Asp408 in the proton translocation pathway of multidrug transporter AcrB: Evidence from site-directed mutagenesis and carbodiimide labeling. Biochemistry.
9. Su CC, Li M, Gu R, Takatsuka Y, McDermott G, et al. (2006) Conformation of the AcrB multidrug efflux pump in mutants of the putative proton relay pathway. J Bacteriol 188: 7290-7296.
10. Stroebel D, Sendra V, Cannella D, Helbig K, Nies DH, et al. (2007) Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent. Biochim Biophys Acta 1768: 1567-1573.
11. Lemieux MJ, Song J, Kim MJ, Huang Y, Villa A, et al. (2003) Three-dimensional crystallization of the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily. Protein Sci 12: 2748-2756.
12. Nieves-Alicea R, Focia PJ, Craig SP 3rd, Eakin AE (1998) Limited proteolysis of a trypanosomal hypoxanthine phosphoribosyltransferase yields crystals that diffract X-rays to near atomic resolution. Biochim Biophys Acta 1388: 500-505.
13. Kiley P, Zhao X, Vaughn M, Baldo MA, Bruce BD, et al. (2005) Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time. PLoS Biol 3: e230.
14. Vauthey S, Santoso S, Gong H, Watson N, Zhang S (2002) Molecular self-assembly of surfactant-like peptides to form nanotubes and nanovesicles. Proc Natl Acad Sci U S A 99: 5355-5360.
15. von Maltzahn G, Vauthey S, Santoso S, Zhang S (2003) Positively charged surfactant-like peptides self-assemble into nanostructures. Langmuir 19: 4332-4337.
16. Yeh JI, Du S, Tortajada A, Paulo J, Zhang S (2005) Peptergents: peptide detergents that improve stability and functionality of a membrane protein, glycerol-3-phosphate dehydrogenase. Biochemistry 44: 16912-16919.
17. Zhao X, Nagai Y, Reeves PJ, Kiley P, Khorana HG, et al. (2006) Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin. Proc Natl Acad Sci U S A 103: 17707-17712.
18. Popot JL, Berry EA, Charvolin D, Creuzenet C, Ebel C, et al. (2003) Amphipols: polymeric surfactants for membrane biology research. Cell Mol Life Sci 60: 1559-1574.
19. Breyton C, Chabaud E, Chaudier Y, Pucci B, Popot JL (2004) Hemifluorinated surfactants: a non-dissociating environment for handling membrane proteins in aqueous solutions? FEBS Lett 564: 312-318.
20. Chabaud E, Barthelemy P, Mora N, Popot JL, Pucci B (1998) Stabilization of integral membrane proteins in aqueous solution using fluorinated surfactants. Biochimie 80: 515-530.
21. Lebaupain F, Salvay AG, Olivier B, Durand G, Fabiano AS, et al. (2006) Lactobionamide surfactants with hydrogenated, perfluorinated or hemifluorinated tails: physical-chemical and biochemical characterization. Langmuir 22: 8881-8890.
22. Franke S, Grass G, Nies DH (2001) The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions. Microbiology 147: 965-972.
23. Massenet C, Chenavas S, Cohen-Addad C, Dagher MC, Brandolin G, et al. (2005) Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains. J Biol Chem 280: 13752-13761.
24. Sendra V, Cannella D, Bersch B, Fieschi F, Menage S, et al. (2006) CopH from Cupriavidus metallidurans CH34. A novel periplasmic copper-binding protein. Biochemistry 45: 5557-5566.
25. Rosenbaum DM, Cherezov V, Hanson MA, Rasmussen SG, Thian FS, et al. (2007) GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function. Science 318: 1266-1273.
26. Pocanschi CL, Dahmane T, Gohon Y, Rappaport F, Apell HJ, et al. (2006) Amphipathic polymers: tools to fold integral membrane proteins to their active form. Biochemistry 45: 13954-13961.
27. Screpanti E, Padan E, Rimon A, Michel H, Hunte C (2006) Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation. J Mol Biol 362: 192-202.
28. Lu M, Fu D (2007) Structure of the zinc transporter YiiP. Science 317: 1746-1748.
29. Goldberg M, Pribyl T, Juhnke S, Nies DH (1999) Energetics and topology of CzcA, a cation/proton antiporter of the resistance-nodulation-cell division protein family. J Biol Chem 274: 26065-26070.
30. Rich RL, Myszka DG (2000) Advances in surface plasmon resonance biosensor analysis. Curr Opin Biotechnol 11: 54-61.