Detrimental Effects of RNAi: A Cautionary Note on Its Use in Drosophila Ageing Studies

PLoS ONE

Volumn 7, Issue 9, 2012, Pages

  Alic, Nazif ^a   Hoddinott, Matthew P ^a,b   Foley, Andrea ^a   Slack, Cathy ^a   Piper, Matthew D W ^a   Partridge, Linda ^a,b  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b MAX PLANCK INSTITUTE FOR BIOLOGY OF AGEING   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; DICER; DOUBLE STRANDED RNA; GREEN FLUORESCENT PROTEIN;

AGING; ARTICLE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; FEMALE; GENE CONSTRUCT; GENE EXPRESSION; GENE FUNCTION; LIFESPAN; LONGEVITY; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN TARGETING; RNA INTERFERENCE; RNA SEQUENCE; SURVIVAL RATE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DROSOPHILA; FEMALE; GREEN FLUORESCENT PROTEINS; MALE; RNA INTERFERENCE; RNA, DOUBLE-STRANDED;

CAENORHABDITIS ELEGANS; DROSOPHILA MELANOGASTER;

EID: 84866460549     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0045367     Document Type: Article

References (28)

1. Christensen K, Doblhammer G, Rau R, Vaupel JW, (2009) Ageing populations: the challenges ahead. Lancet 374: 1196-1208.
2. Fontana L, Partridge L, Longo VD, (2010) Extending healthy life span-from yeast to humans. Science 328: 321-326.
3. Hannon GJ, (2002) RNA interference. Nature 418: 244-251.
4. Bartel DP, (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116: 281-297.
5. Lee SS, Lee RY, Fraser AG, Kamath RS, Ahringer J, et al. (2003) A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nat Genet 33: 40-48.
6. Hamilton B, Dong Y, Shindo M, Liu W, Odell I, et al. (2005) A systematic RNAi screen for longevity genes in C. elegans. Genes Dev 19: 1544-1555.
7. Panowski SH, Wolff S, Aguilaniu H, Durieux J, Dillin A, (2007) PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans. Nature 447: 550-555.
8. Copeland JM, Cho J, Lo T Jr, Hur JH, Bahadorani S, et al. (2009) Extension of Drosophila life span by RNAi of the mitochondrial respiratory chain. Curr Biol 19: 1591-1598.
9. Tatar M, Kopelman A, Epstein D, Tu MP, Yin CM, et al. (2001) A mutant Drosophila insulin receptor homolog that extends life-span and impairs neuroendocrine function. Science 292: 107-110.
10. Clancy DJ, Gems D, Harshman LG, Oldham S, Stocker H, et al. (2001) Extension of life-span by loss of CHICO, a Drosophila insulin receptor substrate protein. Science 292: 104-106.
11. Ikeya T, Broughton S, Alic N, Grandison R, Partridge L, (2009) The endosymbiont Wolbachia increases insulin/IGF-like signalling in Drosophila. Proc Biol Sci 276: 3799-3807.
12. Slack C, Giannakou ME, Foley A, Goss M, Partridge L, (2011) dFOXO-independent effects of reduced insulin-like signaling in Drosophila. Aging Cell 10: 735-748.
13. Roignant JY, Carre C, Mugat B, Szymczak D, Lepesant JA, et al. (2003) Absence of transitive and systemic pathways allows cell-specific and isoform-specific RNAi in Drosophila. RNA 9: 299-308.
14. Osterwalder T, Yoon KS, White BH, Keshishian H, (2001) A conditional tissue-specific transgene expression system using inducible GAL4. Proc Natl Acad Sci U S A 98: 12596-12601.
15. Roman G, Endo K, Zong L, Davis RL, (2001) P[Switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster. Proc Natl Acad Sci U S A 98: 12602-12607.
16. Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, et al. (2005) dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference. Nucleic Acids Res 33: W589-591.
17. Mair W, Piper MD, Partridge L, (2005) Calories do not explain extension of life span by dietary restriction in Drosophila. PLoS Biol 3: e223.
18. Cox DR, (1972) Regression models and life-tables. J R Stat Soc Ser B Stat Methodol 34: 187-220.
19. Poirier L, Shane A, Zheng J, Seroude L, (2008) Characterization of the Drosophila gene-switch system in aging studies: a cautionary tale. Aging Cell 7: 758-770.
20. Moffat J, Reiling JH, Sabatini DM, (2007) Off-target effects associated with long dsRNAs in Drosophila RNAi screens. Trends Pharmacol Sci 28: 149-151.
21. Kulkarni MM, Booker M, Silver SJ, Friedman A, Hong P, et al. (2006) Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nat Methods 3: 833-838.
22. Grimm D, Streetz KL, Jopling CL, Storm TA, Pandey K, et al. (2006) Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 441: 537-541.
23. Liu N, Landreh M, Cao K, Abe M, Hendriks GJ, et al. (2012) The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. Nature 482: 519-523.
24. Fernandez-Ayala DJ, Sanz A, Vartiainen S, Kemppainen KK, Babusiak M, et al. (2009) Expression of the Ciona intestinalis alternative oxidase (AOX) in Drosophila complements defects in mitochondrial oxidative phosphorylation. Cell Metab 9: 449-460.
25. Ford D, Hoe N, Landis GN, Tozer K, Luu A, et al. (2007) Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone. Exp Gerontol 42: 483-497.
26. Giannakou ME, Goss M, Junger MA, Hafen E, Leevers SJ, et al. (2004) Long-lived Drosophila with overexpressed dFOXO in adult fat body. Science 305: 361.
27. Bulow MH, Aebersold R, Pankratz MJ, Junger MA, (2010) The Drosophila FoxA ortholog Fork head regulates growth and gene expression downstream of Target of rapamycin. PLoS One 5: e15171.
28. Bass TM, Grandison RC, Wong R, Martinez P, Partridge L, et al. (2007) Optimization of dietary restriction protocols in Drosophila. J Gerontol A Biol Sci Med Sci 62: 1071-1081.