Detection and identification of bacteria using in-house broad range 16S rDNA PCR amplification and genus-specific DNA hybridization probes, located within variable regions of 16S rRNA genes

APMIS

Volumn 104, Issue 6, 1996, Pages 451-458

  Monstein, Hans Jürg ^a   Kihlström, Erik ^b   Tiveljung, Annika ^b  

^a LINKÖPING UNIVERSITY HOSPITAL   (Sweden)

^b NONE

Author keywords

16S rRNA genes; Chlamydia trachomatis; Diagnostic PCR; Helicobacter pylori; Mobiluncus curtisii

Indexed keywords

ARTICLE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; HELICOBACTER PYLORI; MOBILUNCUS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE; URINE;

EID: 0029834993     PISSN: 09034641     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1699-0463.1996.tb00741.x     Document Type: Article

References (14)

1. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F.: Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75: 4801-4805, 1978.
2. Fischer, S. G. & Lerman, L. S.: DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: Correspondence with melting theory. Proc. Natl. Acad. Sci. USA 80: 1579-1583, 1983.
3. Gray, M. W., Sankoff, D. & Cedergren, R. J.: On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA. Nucleic Acids Res. 12: 5837-5852, 1984.
4. Jaschek, G., Gaydos, C. A., Welsh, L. E. & Quinn, T. C.: Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay. J. Clin. Microbiol. 31: 1209-1212, 1993.
5. Kihlström, E. & Danielsson, D.: Advances in biology, management and prevention of infections caused by Chlamydia trachomatis and Neisseria gonorrhoeae. Curr Opin. Inf. Dis. 7: 25-33, 1994.
6. Lee, H. H., Chernesky, M. A., Schachter, J., Burczak, J. D., Andrews, W. W., Muldoon, S., Leckie, G. & Stamm, W. E.: Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine. Lancet 345: 213-216, 1995.
7. Majeed, M. & Kihlström, E.: Mobilization of Factin and clathrin during redistribution of Chlamydia trachomatis to an intracellular site in eukaryotic cells. Infect. Immun. 59: 4465-4472, 1991.
8. Muyzer, G, & de Waal, E. Determination of the genetic diversity of microbial communities using DGGE analysis of PCR-amplified 16S rDNA. In: Stal, L. J. & Caumette, P. (Eds.): NATO ASI Series, Vol. G 35. Springer-Verlag, Berlin. Heidelberg 1994.
9. Muyzer, G., de Waal, E. C. & Uitterlinden, A. G.: Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Env. Microbiol. 59: 695-700, 1993
10. Relman, D. A., Schmidt, T. M., MacDermott, R. P. & Falkow, S.: Identification of the uncultured bacillus of Whipple's disease. N. Engl. J. Med. 327: 293-301, 1992.
11. Tiveljung, A., Backström, J., Forsum, U. & Monstein, H.-J.: Broad-range PCR amplification and DNA sequence analysis reveals variable motifs in 16S rRNA genes of Mobiluncus species. APMIS 103: 755-763, 1995.
12. Tiveljung, A., Forsum, U. & Monstein, H.-J.: Classification of the genus Mohiluncus based on comparative partial 16S rRNA gene analysis. Int. J. Syst. Bacteriol. 46: 332-336, 1996.
13. Wahlberg, J., Lundeherg, J., Hultman, T. & Uhlén, M.: General colorimetric method for DNA diagnostics allowing direct solid-phase genomic sequencing of the positive samples. Proc. Natl. Acad. Sci. USA 87: 6569-6573, 1990.
14. Woo, T. H. S., Cheng, A. F. & Ling, J. M.: An application of a simple method for the preparation of bacterial DNA. Biotechniques 13: 696-698, 1992.