Deep dermal fibroblasts refractory to migration and decorin-induced apoptosis contribute to hypertrophic scarring

Journal of Burn Care and Research

Volumn 33, Issue 5, 2012, Pages 668-677

  Honardoust, Dariush ^a   Ding, Jie ^a   Varkey, Mathew ^a   Shankowsky, Heather A ^a   Tredget, Edward E ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 8; DECORIN; FIBRONECTIN; HISTONE H1; INTERLEUKIN 1BETA CONVERTING ENZYME; MESSENGER RNA; PROTEIN BAX; PROTEIN P53;

APOPTOSIS; ARTICLE; CELL ADHESION; CELL DEATH; CELL FUNCTION; CELL MIGRATION; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; HYPERTROPHIC SCAR; IMMUNOHISTOCHEMISTRY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN FIBROBLAST; SKIN FIBROSIS;

APOPTOSIS; CASPASE 8; CELL ADHESION; CELL MIGRATION ASSAYS; CICATRIX, HYPERTROPHIC; DECORIN; EXTRACELLULAR MATRIX; EXTRACELLULAR MATRIX PROTEINS; FIBROBLASTS; HUMANS; PROTEOGLYCANS; RNA, MESSENGER; SIGNAL TRANSDUCTION; WOUND HEALING;

EID: 84866421247     PISSN: 1559047X     EISSN: 15590488     Source Type: Journal    
DOI: 10.1097/BCR.0b013e31824088e3     Document Type: Article

References (39)

1. McDougall S, Dallon J, Sherratt J, Maini PK. Fibroblast migration and collagen deposition during dermal wound healing: mathematical modelling and clinical implications. Philos Transact A Math Phys Eng Sci 2006;364:1385-405.
2. Shah M, Foreman DM, Ferguson MW. Neutralisation of TGF-beta 1 and TGF-beta 2 or exogenous addition of TGF-beta 3 to cutaneous rat wounds reduces scarring. J Cell Sci 1995;108:985-1002.
3. Scott PG, Dodd CM, Tredget EE, Ghahary A, Rahemtulla F. Immunohistochemical localization of the proteoglycans decorin, biglycan and versican and transforming growth factor-beta in human post-burn hypertrophic and mature scars. Histopathology 1995;26:423-31.
4. Wang J, Dodd C, Shankowsky HA, Scott PG, Tredget EE; Wound Healing Research Group. Deep dermal fibroblasts contribute to hypertrophic scarring. Lab Invest 2008;88: 1278-90.
5. Nedelec B, Shankowsky H, Scott PG, Ghahary A, Tredget EE. Myofibroblasts and apoptosis in human hypertrophic scars: the effect of interferon-alpha2b. Surgery 2001;130: 798-808.
6. Darby IA, Hewitson TD. Fibroblast differentiation in wound healing and fibrosis. Int Rev Cytol 2007;257:143-79.
7. Desmoulière A, Redard M, Darby I, Gabbiani G. Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar. Am J Pathol 1995;146: 56-66.
8. Desmoulière A. Factors influencing myofibroblast differentiation during wound healing and fibrosis. Cell Biol Int 1995; 19:471-6.
9. Wu H, Wang S, Xue A, et al. Overexpression of decorin induces apoptosis and cell growth arrest in cultured rat mesangial cells in vitro. Nephrology (Carlton) 2008;13: 607-15.
10. Häkkinen L, Strassburger S, Kähäri VM, et al. A role for decorin in the structural organization of periodontal ligament. Lab Invest 2000;80:1869-80.
11. Moulin V. Growth factors in skin wound healing. Eur J Cell Biol 1995;68:1-7.
12. Hsieh P, Chen LB. Behavior of cells seeded in isolated fibronectin matrices. J Cell Biol 1983;96:1208-17.
13. Harper PA, Juliano RL. Two distinct mechanisms of fibroblast adhesion. Nature 1981;290:136-8.
14. Dunkin CS, Pleat JM, Gillespie PH, Tyler MP, Roberts AH, McGrouther DA. Scarring occurs at a critical depth of skin injury: precise measurement in a graduated dermal scratch in human volunteers. Plast Reconstr Surg 2007;119:1722-32; discussion 1733-4.
15. Latham GJ. Normalization of microRNA quantitative RT-PCR data in reduced scale experimental designs. Methods Mol Biol 2010;667:19-31.
16. Xu M, Zhang B, Su X, Zhang S, Huang M. Reference gene selection for quantitative real-time polymerase chain reaction in Populus. Anal Biochem 2011;15408:337-9.
17. Montgomery RB, Moscatello DK, Stahl WL. Differential modulation of mitogen-activated protein (MAP) kinase/extracellular signal-related kinase kinase and MAP kinase activities by a mutant epidermal growth factor receptor. J Biol Chem 1995;270:30562-6.
18. Moscatello DK, Santra M, Mann DM, McQuillan DJ, Wong AJ, Iozzo RV. Decorin suppresses tumor cell growth by activating the epidermal growth factor receptor. J Clin Invest 1998;101:406-12.
19. Santra M, Reed CC, Lozzo RV. Decorin binds to a narrow region of the epidermal growth factor (EGF) receptor, partially overlapping but distinct from the EGF-binding epitope. J Biol Chem 2002;277,35671-81.
20. Reed CC, Waterhouse A, Kirby S, et al. Decorin prevents metastatic spreading of breast cancer. Oncogene 2005;24: 1104-10.
21. Seidler DG, Goldoni S, Agnew C, et al. Decorin protein core inhibits in vivo cancer growth and metabolism by hindering epidermal growth factor receptor function and triggering apoptosis via caspase-3 activation. J Biol Chem 2006;281:26408-18.
22. Hagood JS, Lasky JA, Nesbitt JE, Segarini P. Differential expression, surface binding, and response to connective tissue growth factor in lung fibroblast subpopulations. Chest 2001;120(1 Suppl):64S-6S.
23. Zhou Y, Hagood JS, Murphy-Ullrich JE. Thy-1 expression regulates the ability of rat lung fibroblasts to activate transforming growth factor-beta in response to fibrogenic stimuli. Am J Pathol 2004;165:659-69.
24. Hagood JS, Prabhakaran P, Kumbla P, et al. Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis. Am J Pathol 2005;167:365-79.
25. Scott PG, Dodd CM, Ghahary A, Shen YJ, Tredget EE. Fibroblasts from post-burn hypertrophic scar tissue synthesize less decorin than normal dermal fibroblasts. Clin Sci 1998;94:541-7.
26. Wang J, Hori K, Ding J, et al. Toll-like receptors expressed by dermal fibroblasts contribute to hypertrophic scarring. J Cell Physiol 2011;226:1265-73.
27. Honardoust D, Varkey M, Hori K, Ding J, Shankowsky HA, Tredget EE. Small leucine-rich proteoglycans, decorin and fibromodulin, are reduced in post-burn hypertrophic scar. Wound Repair Regen 2011;19:368-78.
28. Spyrou GE, Watt DA, Naylor IL. The origin and mode of fibroblast migration and proliferation in granulation tissue. Br J Plast Surg 1998;51:455-61.
29. Ghahary A, Shen YJ, Nedelec B, Wang R, Scott PG, Tredget EE. Collagenase production is lower in post-burn hypertrophic scar fibroblasts than in normal fibroblasts and is reduced by insulin-like growth factor-1. J Invest Dermatol 1996;106:476-81.
30. Danielson KG, Baribault H, Holmes DF, Graham H, Kadler KE, Iozzo RV. Targeted disruption of decorin leads to abnormal collagen fibril morphology and skin fragility. J Cell Biol 1997;136:729-43.
31. Zhang Z, Liu Y, Zhang X, Xu WS. The content of decorin and its mRNA expression in normal human skin and hyperplastic scars. Zhonghua Shao Shang Za Zhi 2004;20: 76-8.
32. Zhang Z, Li XJ, Liu Y, Zhang X, Li YY, Xu WS. Recombinant human decorin inhibits cell proliferation and downregulates TGF-beta production in hypertrophic scar fibroblasts. Burns 2007;33:634-41.
33. Sayani K, Dodd CM, Nedelec B, et al. Delayed appearance of decorin in healing burn scars. Histopathology 2000;36:262-72.
34. Häkkinen L, Westermarck J, Kähäri VM, Larjava H. Human granulation-tissue fibroblasts show enhanced proteoglycan gene expression and altered response to TGF-beta 1. J Dent Res 1996;75:1767-78.
35. Schönherr E, Levkau B, Schaefer L, Kresse H, Walsh K. Decorin-mediated signal transduction in endothelial cells. Involvement of Akt/protein kinase B in up-regulation of p21(WAF1/CIP1) but not p27(KIP1). J Biol Chem 2001; 276:40687-92.
36. Battey NH, James NC, Greenland AJ, Brownlee C. The secretory system. Exocytosis and endocytosis. Plant Cell 1999;11:643-60.
37. Hausser H, Ober B, Quentin-Hoffmann E, Schmidt B, Kresse H. Endocytosis of different members of the small chondroitin/dermatan sulfate proteoglycan family. J Biol Chem 1992;267:11559-64.
38. Hausser H, Schönherr E, Müller M, et al. Receptor-mediated endocytosis of decorin: involvement of leucine-rich repeat structures. Arch Biochem Biophys 1998;349: 363-70.
39. Babelova A, Moreth K, Tsalastra-Greul W, et al. Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors. J Biol Chem 2009;284: 24035-48.