Bioengineered human IAS reconstructs with functional and molecular properties similar to intact IAS

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 303, Issue 6, 2012, Pages

  Singh, Jagmohan ^a   Rattan, Satish ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Internal anal sphincter; Internal anal sphincter reconstructs; Protein kinase C; Rectoanal incontinence; Rho kinase; Smooth muscle tone

Indexed keywords

2 [1 (3 DIMETHYLAMINOPROPYL) 3 INDOLYL] 3 (3 INDOLYL)MALEIMIDE; 4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ALPHA ACTIN; BETHANECHOL; CALCIUM; ISOPRENALINE; MYOSIN LIGHT CHAIN; PHORBOL DIBUTYRATE; POTASSIUM CHLORIDE; POTASSIUM ION; PROTEIN CPI 17; PROTEIN KINASE C ALPHA; PROTEIN MYPT1; RHO FACTOR; RHO KINASE; UNCLASSIFIED DRUG;

ANUS SPHINCTER; ARTICLE; BIOENGINEERING; CELL DIFFERENTIATION; CONTROLLED STUDY; DEPOLARIZATION; FEASIBILITY STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; MUSCLE TONE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SMOOTH MUSCLE FIBER; TISSUE CULTURE; WESTERN BLOTTING;

ADRENERGIC BETA-AGONISTS; AMIDES; ANAL CANAL; BETHANECHOL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; INDOLES; ISOPROTERENOL; MALEIMIDES; MUSCARINIC AGONISTS; MUSCLE TONUS; MUSCLE, SMOOTH; PHORBOL 12,13-DIBUTYRATE; POTASSIUM; PROTEIN KINASE C; PYRIDINES; RHO-ASSOCIATED KINASES; TISSUE ENGINEERING;

EID: 84866366248     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.00112.2012     Document Type: Article

References (39)

1. Ali I, Sarna SK. Selective modulation of PKC isozymes by inflammation in canine colonic circular muscle cells. Gastroenterology 122: 483-494, 2002.
2. Bhardwaj R, Vaizey CJ, Boulos PB, Hoyle CHV. Neuromyogenic properties of the internal anal sphincter: therapeutic rationale for anal fissures. Gut 46: 861-868, 2000.
3. Bharucha AE. Outcome measures for fecal incontinence: anorectal structure and function. Gastroenterology 126: S90-S98, 2004.
4. Bi D, Nishimura J, Niiro N, Hirano K, Kanaide H. Contractile properties of the cultured vascular smooth muscle cells: the crucial role played by RhoA in the regulation of contractility. Circ Res 96: 890-897, 2005.
5. Biancani P, Walsh JH, Behar J. Vasoactive intestinal polypeptide: a neurotransmitter for relaxation of the rabbit internal anal sphincter. Gastroenterology 89: 867-874, 1985.
6. Birukov KG, Frid MG, Rogers JD, Shirinsky VP, Koteliansky VE, Campbell JH, Campbell GR. Synthesis and expression of smooth muscle phenotype markers in prmary culture of rabbit aortic smooth muscle cells: influence of seeding density and media and relation to cell contractility. Exp Cell Res 204: 46-53, 1993.
7. Burleigh DE, D'Mello A. Neural and pharmacological factors affecting motility of the internal anal sphincter. Gastroenterology 84: 409-417, 1983.
8. Chakder S, Sarma D, Rattan S. Mechanism of internal anal sphincter smooth muscle relaxation by phorbol 12,13-dibutyrate. Am J Physiol Gastrointest Liver Physiol 280: G1341-G1350, 2001.
9. Cummings CL, Gawlitta D, Nerem RM, Stegemann JP. Properties of engineered vascular constructs made from collagen, fibrin, and collagenfibrin mixtures. Biomaterials 25: 3699-3707, 2004.
10. De Godoy MA, Rattan S. Role of rho kinase in the functional and dysfunctional tonic smooth muscles. Trends Pharmacol Sci 32: 384-393, 2011.
11. Eto M, Kitazawa T, Yazawa A, Mukai H, Ono Y, Brautigan DL. Histamine-induced vasoconstriction involves phosphorylation of a specific inhibitor protein for myosin phosphatase by protein kinase C α and δ isoforms. J Biol Chem 276: 29072-29078, 2001.
12. Halayko AJ, Salari H, Ma X, Stephens NL. Markers of airway smooth muscle cell phenotype. Am J Physiol Lung Cell Mol Physiol 270: L1040-L1051, 1996.
13. Harnett KM, Cao W, Biancani P. Signal-transduction pathways that regulate smooth muscle function I. Signal transduction in phasic (esophageal) and tonic (gastroesophageal sphincter) smooth muscles. Am J Physiol Gastrointest Liver Physiol 288: G407-G416, 2005.
14. Harris LJ, Abdollahi H, Zhang P, McIlhenny S, Tulenko TN, DiMuzio PJ. Differentiation of adult stem cells into smooth muscle for vascular tissue engineering. J Surg Res 168: 306-314, 2011.
15. Hecker L, Baar K, Dennis RG, Bitar KN. Development of a threedimensional physiological model of the internal anal sphincter bioengineered in vitro from isolated smooth muscle cells. Am J Physiol Gastrointest Liver Physiol 289: G188-G196, 2005.
16. Hong H, Stegemann JP. 2D and 3D collagen and fibrin biopolymers promote specific ECM and integrin gene expression by vascular smooth muscle cells. J Biomater Sci Polym Ed 19: 1279-1293, 2008.
17. Jiang H, Halayko AJ, Rao K, Cunningham P, Stephens NL. Normalization of force generated by canine airway smooth muscles. Am J Physiol Lung Cell Mol Physiol 260: L522-L529, 1991.
18. Madalinski M, Kalinowski L. Novel options for the pharmacological treatment of chronic anal fissure-Role of botulinum toxin. Curr Clin Pharmacol 4: 47-52, 2009.
19. Matsumoto T, Nishiyama M, Kobayashi T, Kasuya Y, Kamata K. Effect of phorbol 12,13-dibutyrate on smooth muscle tone in rat stomach fundus. J Smooth Muscle Res 41: 107-116, 2005.
20. Mitsui M, Karaki H. Contractile and relaxant effects of phorbol ester in the intestinal smooth muscle of guinea-pig taenia caeci. Br J Pharmacol 109: 229-233, 1993.
21. Moummi C, Rattan S. Effect of methylene blue and N-ethylmaleimide on internal anal sphincter relaxation. Am J Physiol Gastrointest Liver Physiol 255: G571-G578, 1988.
22. Nair DG, Han TY, Lourenssen S, Blennerhassett MG. Proliferation modulates intestinal smooth muscle phenotype in vitro and in colitis in vivo. Am J Physiol Gastrointest Liver Physiol 300: G903-G913, 2011.
23. Oishi K, Itoh Y, Isshiki Y, Kai C, Takeda Y, Yamaura K, Takano-Ohmuro H, Uchida MK. Agonist-induced isometric contraction of smooth muscle cell-populated collagen gel fiber. Am J Physiol Cell Physiol 279: C1432-C1442, 2000.
24. Park SY, Song HJ, Sohn UD. Participation of Rho-associated kinase in electrical stimulated and acetylcholine-induced contraction of feline esophageal smooth muscle. Eur J Pharmacol 607: 220-225, 2009.
25. Patel CA, Rattan S. Spontaneously tonic smooth muscle has characteristically higher levels of RhoA/ROK compared with the phasic smooth muscle. Am J Physiol Gastrointest Liver Physiol 291: G830-G837, 2006.
26. Patel CA, Rattan S. Cellular regulation of basal tone in internal anal sphincter smooth muscle by RhoA/ROCK. Am J Physiol Gastrointest Liver Physiol 292: G1747-G1756, 2007.
27. Percy WH. In vitro techniques for the study of gastrointestinal motility. In: Handbook of Methods in Gastrointestinal Pharmacology, edited by Gaginella TS. Boca Raton, FL: CRC, 1996, p. 189-224.
28. Raghavan S, Gilmont RR, Miyasaka EA, Somara S, Srinivasan S, Teitelbaum DH, Bitar KN. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141: 310-319, 2011.
29. Raghavan S, Miyasaka EA, Hashish M, Somara S, Gilmont RR, Teitelbaum DH, Bitar KN. Successful implantation of physiologically functional bioengineered mouse internal anal sphincter. Am J Physiol Gastrointest Liver Physiol 299: G430-G439, 2010.
30. Rao SS. Pathophysiology of adult fecal incontinence. Gastroenterology 126: S14-S22, 2004.
31. Rattan S, Benjamin P, Maxwell IVPJ. RhoA/ROCK-kinase: pathophysiologic and therapeutic implications in gastrointestinal smooth muscle tone and relaxation. Gastroenterology 138: 13-18, 2010.
32. Rattan S, Singh J. RhoA/ROCK pathway is the major molecular determinant of basal tone in intact human internal anal sphincter. Am J Physiol Gastrointest Liver Physiol 302: G664-G675, 2012.
33. Sims SM, Chrones T, Preiksaitis HG. Calcium sensitization in human esophageal muscle: role for RhoA kinase in maintenance of lower esophageal sphincter tone. J Pharmacol Exp Ther 327: 178-186, 2008.
34. Singh J, Maxwell IVPJ, Rattan S. Immunocytochemical evidence for PDBu-induced activation of RhoA/ROCK in human internal anal sphincter smooth muscle cells. Am J Physiol Gastrointest Liver Physiol 301: G317-G325, 2011.
35. Singh J, Mehendiratta V, Del Galdo F, Jimenez SA, Cohen S, DiMarino AJ, Rattan S. Immunoglobulins from scleroderma patients inhibit the muscarinic receptor activation in internal anal sphincter smooth muscle cells. Am J Physiol Gastrointest Liver Physiol 297: G1206-G1213, 2009.
36. Somara S, Bitar KN. Direct association of calponin with specific domains of PKC-α. Am J Physiol Gastrointest Liver Physiol 295: G1246-G1254, 2008.
37. Somara S, Gilmont RR, Dennis RG, Bitar KN. Bioengineered internal anal sphincter derived from isolated human internal anal sphincter smooth muscle cells. Gastroenterology 137: 53-61, 2009.
38. Woodsome TP, Polzin A, Kitazawa K, Eto M, Kitazawa T. Agonistand depolarization-induced signals for myosin light chain phosphorylation and force generation of cultured vascular smooth muscle cells. J Cell Sci 119: 1769-1780, 2006.
39. Worth NF, Campbell GR, Campbell JH, Rolfe BE. Rho expression and activation in vascular smooth muscle cells. Cell Motil Cytoskeleton 59: 189-200, 2004.