Staurosporine-induced differentiation of the RGC-5 cell line leads to apoptosis and cell death at the lowest differentiating concentration

Graefe's Archive for Clinical and Experimental Ophthalmology

Volumn 250, Issue 8, 2012, Pages 1221-1229

  Schultheiss, Maximillian ^a   Schnichels, Sven ^a   Miteva, Kapka ^b   Warstat, Katrin ^b   Szurman, Peter ^a   Spitzer, Martin S ^a   Van Linthout, Sophie ^b  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Apoptosis; Cell death; Differentiation; Retinal ganglion cells; RGC 5; Staurosporine

Indexed keywords

7 AMINODACTINOMYCIN; CASPASE 3; CASPASE 7; LIPOCORTIN 5; PROPIDIUM IODIDE; STAUROSPORINE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CELL DENSITY; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETINA GANGLION CELL; RGC 5 CELL;

ANNEXIN A5; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CASPASE 3; CASPASE 7; CELL DEATH; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; DACTINOMYCIN; ENZYME INHIBITORS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; POLYMERASE CHAIN REACTION; PROTO-ONCOGENE PROTEINS C-BCL-2; RETINAL GANGLION CELLS; RNA, MESSENGER; STAUROSPORINE;

EID: 84866363317     PISSN: 0721832X     EISSN: 1435702X     Source Type: Journal    
DOI: 10.1007/s00417-011-1906-3     Document Type: Article

References (23)

1. Krishnamoorthy RR, Agarwal P, Prasanna G, Vopat K, LambertW, Sheedlo HJ, Pang IH, Shade D, Wordinger RJ, Yorio T, Clark AF, Agarwal N (2001) Characterization of a transformed rat retinal ganglion cell line. Brain Res Mol Brain Res 86:1-12
2. Moorhouse AJ, Li S, Vickery RM, Hill MA, Morley JW (2004) A patch-clamp investigation of membrane currents in a novel mammalian retinal ganglion cell line. Brain Res 1003:205-208
3. Van Bergen NJ, Wood JP, Chidlow G, Trounce IA, Casson RJ, Ju WK, Weinreb RN, Crowston JG (2009) Recharacterization of the RGC-5 retinal ganglion cell line. Invest Ophthalmol Vis Sci 50:4267-4272
4. Wood JP, Chidlow G, Tran T, Crowston J, Casson RJ (2010) A comparison of differentiation protocols for Rgc-5 cells. Invest Ophthalmol Vis Sci 51(7):3774-3783
5. Frassetto LJ, Schlieve CR, Lieven CJ, Utter AA, Jones MV, Agarwal N, Levin LA (2006) Kinase-dependent differentiation of a retinal ganglion cell precursor. Invest Ophthalmol Vis Sci 47:427-438
6. Schallenberg M, Charalambous P, Thanos S (2009) GM-CSF regulates the ERK1/2 pathways and protects injured retinal ganglion cells from induced death. Exp Eye Res 89:665-677
7. Schwechter BR, Millet LE, Levin LA (2007) Histone deacetylase inhibition-mediated differentiation of RGC-5 cells and interaction with survival. Invest Ophthalmol Vis Sci 48:2845-2857
8. Ganapathy PS, Dun Y, Ha Y, Duplantier J, Allen JB, Farooq A, Bozard BR, Smith SB (2010) Sensitivity of staurosporine-induced differentiated RGC-5 cells to homocysteine. Curr Eye Res 35:80-90
9. Lieven CJ, Millet LE, Hoegger MJ, Levin LA (2007) Induction of axon and dendrite formation during early RGC-5 cell differentiation. Exp Eye Res 85:678-683
10. Nieto PS, Acosta-Rodriguez VA, Valdez DJ, Guido ME (2010) Differential responses of the mammalian retinal ganglion cell line RGC-5 to physiological stimuli and trophic factors. Neurochem Int 57:216-226
11. Schnichels S, Heiduschka P, Julien S (2011) RGMA and neogenin protein expression are influenced by lens injury following optic nerve crush in the rat retina. Graefes Arch Clin Exp Ophthalmol; Epub ahead of print
12. Schnichels S, Heiduschka P, Julien S (2011) Different spatial and temporal protein expressions of repulsive guidance molecule a and neogenin in the rat optic nerve after optic nerve crush with and without lens injury. J Neurosci Res 89:490-505
13. Heiduschka P, Schnichels S, Fuhrmann N, Hofmeister S, Schraermeyer U, Wissinger B, Alavi MV (2010) Electrophysiological and histologic assessment of retinal ganglion cell fate in a mouse model for OPA1-associated autosomal dominant optic atrophy. Invest Ophthalmol Vis Sci 51:1424-1431
14. Van Linthout S, Spillmann F, Riad A, Trimpert C, Lievens J, Meloni M, Escher F, Filenberg E, Demir O, Li J, Shakibaei M, Schimke I, Staudt A, Felix SB, Schultheiss HP, De Geest B, Tschope C (2008) Human apolipoprotein A-I gene transfer reduces the development of experimental diabetic cardiomyopathy. Circulation 117:1563-1573
15. Harper MM, Adamson L, Blits B, Bunge MB, Grozdanic SD, Sakaguchi DS (2009) Brain-derived neurotrophic factor released from engineered mesenchymal stem cells attenuates glutamateand hydrogen peroxide-mediated death of staurosporinedifferentiated RGC-5 cells. Exp Eye Res 89:538-548
16. Thompson AF, Levin LA (2010) Neuronal differentiation by analogs of staurosporine. Neurochem Int 56:554-560
17. Marzo I, Brenner C, Zamzami N, Jurgensmeier JM, Susin SA, Vieira HL, Prevost MC, Xie Z, Matsuyama S, Reed JC, Kroemer G (1998) Bax and adenine nucleotide translocator cooperate in the mitochondrial control of apoptosis. Science 281:2027-2031
18. Antonsson B, Montessuit S, Sanchez B, Martinou JC (2001) Bax is present as a high molecular weight oligomer/complex in the mitochondrial membrane of apoptotic cells. J Biol Chem 276:11615-11623
19. Antonsson B, Montessuit S, Lauper S, Eskes R, Martinou JC (2000) Bax oligomerization is required for channel-forming activity in liposomes and to trigger cytochrome c release from mitochondria. Biochem J 345(Pt 2):271-278
20. Broughton BR, Reutens DC, Sobey CG (2009) Apoptotic mechanisms after cerebral ischemia. Stroke 40:e331-e339
21. Love S (2003) Apoptosis and brain ischaemia. Prog Neuropsychopharmacol Biol Psychiatry 27:267-282
22. Aoun P, Simpkins JW, Agarwal N (2003) Role of PPAR-gamma ligands in neuroprotection against glutamate-induced cytotoxicity in retinal ganglion cells. Invest Ophthalmol Vis Sci 44:2999-3004
23. Harvey R, Chintala SK (2007) Inhibition of plasminogen activators attenuates the death of differentiated retinal ganglion cells and stabilizes their neurite network in vitro. Invest Ophthalmol Vis Sci 48:1884-1891