Trenbolone causes mortality and altered sexual differentiation in Xenopus tropicalis during larval development

Environmental Toxicology and Chemistry

Volumn 31, Issue 10, 2012, Pages 2391-2398

  Olmstead, Allen W ^a   Kosian, Patricia A ^a   Johnson, Rodney ^a   Blackshear, Pamela E ^b   Haselman, Jonathan ^a   Blanksma, Chad ^a   Korte, Joseph J ^a   Holcombe, Gary W ^a   Burgess, Emily ^a   Lindberg Livingston, Annelie ^a   Bennett, Blake A ^a   Woodis, Kacie K ^a   Degitz, Sigmund J ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b INTEGRATED LABORATORY SYSTEMS   (United States)

Author keywords

Amphibians; Androgens; Endocrine disruptors; Hormone mimics

Indexed keywords

AMPHIBIANS; ANDROGENS; AQUATIC SYSTEM; BODY SIZES; CATTLE PRODUCTION; ENDOCRINE DISRUPTOR; LARVAL DEVELOPMENT; MORPHOLOGICAL OBSERVATIONS; POTENTIAL EFFECTS; SEX CHARACTERISTICS; SEX RATIOS; TRENBOLONE; TROPICALIS; XENOPUS;

ANTHROPOMETRY; PATHOLOGY;

EXPERIMENTS;

TRENBOLONE;

ANDROGEN; ANIMAL HUSBANDRY; BODY SIZE; CONCENTRATION (COMPOSITION); DOSE-RESPONSE RELATIONSHIP; ECOTOXICOLOGY; ENDOCRINE DISRUPTOR; EXPERIMENTAL STUDY; FROG; HISTOLOGY; HORMONE; LARVAL DEVELOPMENT; METAMORPHOSIS; MORPHOLOGY; MORTALITY; POLLUTION EFFECT; POLLUTION EXPOSURE; SECONDARY SEXUAL CHARACTERISTICS; SEX RATIO; SEX-RELATED DIFFERENCE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BODY MASS; BODY SIZE; DRUG FATALITY; EMBRYONIC STRUCTURES; FEMALE; HISTOPATHOLOGY; LARVAL DEVELOPMENT; MALE; METAMORPHOSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SEX DIFFERENTIATION; SEX RATIO; TADPOLE; XENOPUS TROPICALIS;

ANABOLIC AGENTS; ANIMALS; BODY SIZE; FEMALE; GONADS; LARVA; MALE; METAMORPHOSIS, BIOLOGICAL; SEX DIFFERENTIATION; SEX RATIO; TOXICITY TESTS, SUBACUTE; TRENBOLONE ACETATE; WATER POLLUTANTS, CHEMICAL; XENOPUS;

AMPHIBIA; ANIMALIA; ANURA; BOS; WOLFFIA; XENOPUS TROPICALIS;

EID: 84866361030     PISSN: 07307268     EISSN: 15528618     Source Type: Journal    
DOI: 10.1002/etc.1965     Document Type: Article

References (40)

1. Pottier J, Cousty C, Heitzman RJ, Reynolds IP. 1981. Differences in the biotransformation of a 17 beta-hydroxylated steroid, trenbolone acetate, in rat and cow. Xenobiotica 11: 489-500.
2. Bauer ER, Daxenberger A, Petri T, Sauerwein H, Meyer HH. 2000. Characterisation of the affinity of different anabolics and synthetic hormones to the human androgen receptor, human sex hormone binding globulin and to the bovine progestin receptor. APMIS 108: 838-846.
3. Blake LS, Martinovic D, Gray LE Jr, Wilson VS, Regal RR, Villeneuve DL, Ankley GT. 2010. Characterization of the androgen-sensitive MDA-kb2 cell line for assessing complex environmental mixtures. Environ Toxicol Chem 29: 1367-1376.
4. Jensen KM, Makynen EA, Kahl MD, Ankley GT. 2006. Effects of the feedlot contaminant 17α-trenbolone on reproductive endocrinology of the fathead minnow. Environ Sci Technol 40: 3112-3117.
5. Schiffer B, Daxenberger A, Meyer K, Meyer HH. 2001. The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle: Environmental studies. Environ Health Perspect 109: 1145-1151.
6. Durhan EJ, Lambright CS, Makynen EA, Lazorchak J, Hartig PC, Wilson VS, Gray LE, Ankley GT. 2006. Identification of metabolites of trenbolone acetate in androgenic runoff from a beef feedlot. Environ Health Perspect 114(Suppl 1): 65-68.
7. Galvez JI, Mazik PM, Phelps RP, Mulvaney DR. 1995. Masculinzination of channel catfish Ictalurus punctatus by oral administration of trenbolone acetate. J World Aquaculture Soc 26: 378-383.
8. Galvez JI, Morrison JR, Phelps RP. 1996. Efficacy of trenbolone acetate in sex inversion of the blue tilapia Oreochromis aureus. J World Aquaculture Soc 27: 483-486.
9. Arslan T, Phelps RP. 2004. Production of monosex male black crappie, Pomoxis nigromaculatus, populations by multiple androgen immersion. Aquaculture 234: 561-573.
10. Zamora HS, Hernandez AA, Herrera SM, Pena EM. 2008. Anabolic and androgenic effect of steroid trenbolone acetate on guppy (Poecilia reticulata). Vet Mex 39: 269-277.
11. Orn S, Yamani S, Norrgren L. 2006. Comparison of vitellogenin induction, sex ratio, and gonad morphology between zebrafish and Japanese medaka after exposure to 17alpha-ethinylestradiol and 17beta-trenbolone. Arch Environ Contam Toxicol 51: 237-243.
12. Holbech H, Kinnberg K, Petersen GI, Jackson P, Hylland K, Norrgren L, Bjerregaard P. 2006. Detection of endocrine disrupters: Evaluation of a fish sexual development test (FSDT). Comp Biochem Physiol C Toxicol Pharmacol 144: 57-66.
13. Morthorst JE, Holbech H, Bjerregaard P. 2010. Trenbolone causes irreversible masculinization of zebrafish at environmentally relevant concentrations. Aquat Toxicol 98: 336-343.
14. Sone K, Hinago M, Itamoto M, Katsu Y, Watanabe H, Urushitani H, Tooi O, Guillette LJJ, Iguchi T. 2005. Effects of an androgenic growth promoter 17beta-trenbolone on masculinization of mosquitofish (Gambusia affinis affinis). Gen Comp Endocrinol 143: 151-160.
15. Ankley GT, Jensen KM, Makynen EA, Kahl MD, Korte JJ, Hornung MW, Henry TR, Denny JS, Leino RL, Wilson VS, Cardon MC, Hartig PC, Gray LE. 2003. Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnow. Environ Toxicol Chem 22: 1350-1360.
16. Seki M, Fujishima S, Nozaka T, Maeda M, Kobayashi K. 2006. Comparison of response to 17beta-estradiol and 17beta-trenbolone among three small fish species. Environ Toxicol Chem 25: 2742-2752.
17. Hayes TB. 1998. Sex determination and primary sex differentiation in amphibians: Genetic and developmental mechanisms. J Exp Zool 281: 373-399.
18. Wallace H, Badawy GM, Wallace BM. 1999. Amphibian sex determination and sex reversal. Cell Mol Life Sci 55: 901-909.
19. Chang CY, Witshci E. 1956. Genic control and hormonal reversal of sex differentiation in Xenopus. Proc Soc Exp Biol Med 93: 140-144.
20. Gallien L. 1956. Inversion experimentale du sexe chez un anoure inferieur Xenopus laevis Daudin. Analyse des consequences genetiques. Bull Biol Fr Belg 90: 163-183.
21. Nieuwkoop PD, Faber J, eds. 1994. Normal Table of Xenopus laevis (Daudin). Garland, New York, NY, USA.
22. Olmstead AW, Korte JJ, Woodis KK, Bennett BA, Ostazeski S, Degitz SJ. 2009. Reproductive maturation of the tropical clawed frog Xenopus tropicalis. Gen Comp Endocrinol 160: 117-123.
23. R Development Core Team. 2009. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
24. Zar J. 1996. Biostatistical Analysis, 3rd ed. Prentice-Hall, Upper Saddle River, NJ, USA.
25. Ogielska M, Kotusz A. 2004. Pattern and rate of ovary differentiation with reference to somatic development in anuran amphibians. J Morphol 259: 41-54.
26. Robertson JC, Watson JT, Kelley DB. 1994. Androgen directs sexual differentiation of laryngeal innervation in developing Xenopus laevis. J Neurobiol 25: 1625-1636.
27. Kelley DB. 1996. Sexual differentiation in Xenopus laevis. In Tinsely RC, Kobel HR, eds, The Biology of Xenopus. Oxford University Press, New York, NY, USA.
28. Wells KD. 2007. The Ecology and Behavior of Amphibians. University of Chicago Press, Chicago, IL, USA.
29. Kelley DB, Pfaff DW. 1976. Hormone effects on male sex behavior in adult South African clawed frogs, Xenopus laevis. Horm Behav 7: 159-182.
30. Bogi C, Levy G, Lutz I, Kloas W. 2002. Functional genomics and sexual differentiation in amphibians. Comp Biochem Physiol B Biochem Mol Biol 133: 559-570.
31. Norris DO, ed. 1996. Vertebrate Endocrinology, 3rd ed. Academic, San Diego, CA, USA.
32. Norris DO, Carr JA, Summers CH, Featherston R. 1997. Interactions of androgens and estradiol on sex accessory ducts of larval tiger salamanders, Ambystoma tigrinum. Gen Comp Endocrinol 106: 348-355.
33. Orlando EF, Kolok AS, Binzcik GA, Gates JL, Horton MK, Lambright CS, Gray LE Jr, Soto AM, Guillette LJ Jr. 2004. Endocrine-disrupting effects of cattle feedlot effluent on an aquatic sentinel species, the fathead minnow. Environ Health Perspect 112: 353-358.
34. Soto AM, Calabro JM, Prechtl NV, Yau AY, Orlando EF, Daxenberger A, Kolok AS, Guillette LJ Jr, le Bizec B, Lange IG, Sonnenschein C. 2004. Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in eastern Nebraska, USA. Environ Health Perspect 112: 346-352.
35. Howell WM, Black DA, Bortone SA. 1980. Abnormal expression of secondary sex characters in a population of mosquitofish, Gambusia affinis holbrooki: Evidence for environmentally-induced masculinization. Copeia 1980: 676-681.
36. Toft G, Baatrup E, Guillette LJ Jr. 2004. Altered social behavior and sexual characteristics in mosquitofish (Gambusia holbrooki) living downstream of a paper mill. Aquat Toxicol 70: 213-222.
37. Parks LG, Lambright CS, Orlando EF, Guillette LJ Jr, Ankley GT, Gray LE Jr. 2001. Masculinization of female mosquitofish in kraft mill effluent-contaminated Fenholloway River water is associated with androgen receptor agonist activity. Toxicol Sci 62: 257-267.
38. Durhan EJ, Lambright C, Wilson V, Butterworth BC, Kuehl OW, Orlando EF, Guillette LJ Jr, Gray LE, Ankley GT. 2002. Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluent. Environ Toxicol Chem 21: 1973-1976.
39. Larsson DGJ, Hallman H, Forlin L. 2000. More male fish embryos near a pulp mill. Environ Toxicol Chem 19: 2911-2917.
40. Larsson DG, Forlin L. 2002. Male-biased sex ratios of fish embryos near a pulp mill: Temporary recovery after a short-term shutdown. Environ Health Perspect 110: 739-742.