Enhancement of the structural stability of full-length clostridial collagenase by calcium ions

Applied and Environmental Microbiology

Volumn 78, Issue 16, 2012, Pages 5839-5844

  Ohbayashi, Naomi ^a,b   Yamagata, Noriko ^c   Goto, Masafumi ^b   Watanabe, Kimiko ^b   Yamagata, Youhei ^b,c   Murayama, Kazutaka ^b  

^a IWAKI MEISEI UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM IONS; COLLAGEN BINDING; COLLAGENASE; CONFORMATIONAL CHANGE; DYNAMIC LIGHT SCATTERING MEASUREMENT; ENZYMATIC ACTIVITIES; FLUORIMETRY; INTER-DOMAIN; MULTIDOMAIN PROTEINS; N-TERMINALS; PROTEIN FLEXIBILITY; PURIFIED PROTEIN; STRUCTURAL STABILITIES; THERMAL MELTING;

AMINO ACIDS; CHELATION; COLLAGEN; ESCHERICHIA COLI; MELTING POINT; PURIFICATION; RECOMBINANT PROTEINS; STABILITY;

CALCIUM;

AZO COMPOUND; AZOCOLL; CALCIUM; CLOSTRIDIOPEPTIDASE A; COLLAGEN; ION; RECOMBINANT PROTEIN;

CALCIUM; CHELATION; CHROMATOGRAPHY; COLIFORM BACTERIUM; ENZYME ACTIVITY; FLUOROMETRY; HYDROLYSIS; ION; MELTING; PROTEIN; RECOMBINATION; SUBSTRATE;

ARTICLE; CHEMISTRY; CLOSTRIDIUM HISTOLYTICUM; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; FLUOROMETRY; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; PROTEIN CONFORMATION; PROTEIN STABILITY; TEMPERATURE; TRANSITION TEMPERATURE;

AZO COMPOUNDS; CALCIUM; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CLOSTRIDIUM HISTOLYTICUM; COLLAGEN; ENZYME STABILITY; ESCHERICHIA COLI; FLUOROMETRY; GENE EXPRESSION; IONS; MICROBIAL COLLAGENASE; PROTEIN CONFORMATION; PROTEIN STABILITY; RECOMBINANT PROTEINS; TEMPERATURE; TRANSITION TEMPERATURE;

CLOSTRIDIUM HISTOLYTICUM; ESCHERICHIA COLI;

EID: 84866276999     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00808-12     Document Type: Article

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