Adjustment of the ratio between collagenase class II and I improves islet isolation outcome

Transplantation Proceedings

Volumn 37, Issue 8, 2005, Pages 3450-3451

  Brandhorst, D ^a,b   Huettler, S ^b   Alt, A ^a   Raemsch Guenther, N ^b   Kurfuerst, M ^c   Bretzel, R G ^a   Brandhorst, H ^a  

^a UNIVERSITY HOSPITAL GIESSEN   (Germany)

^b SERVA Electrophoresis GmbH   (Germany)

^c Nordmark Arzneimittel GmbH and Co KG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGENASE; COLLAGENASE I; COLLAGENASE II; GLUCOSE; INSULIN; NEUTRAL PROTEINASE; TRYPAN BLUE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; CELL ISOLATION; CELL VIABILITY; CONFERENCE PAPER; CONTROLLED STUDY; DIABETES MELLITUS; DIGESTION; INSULIN RELEASE; MALE; MOUSE; NONHUMAN; NUDE MOUSE; PANCREAS ISLET CELL; PANCREAS ISLET TRANSPLANTATION; PRIORITY JOURNAL; QUALITY CONTROL; RAT; RAT STRAIN;

ANIMALS; BIOLOGICAL MARKERS; CELL SEPARATION; COLLAGENASES; ISLETS OF LANGERHANS; RATS;

EID: 27844531629     PISSN: 00411345     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.transproceed.2005.09.079     Document Type: Conference Paper

References (7)

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4. G.H. Vos-Scheperkeuter, Suylichem P.T. van, M.W. Vonk Histochemical analysis of the role of class I and class II Clostridium histolyticum collagenase in the degradation of rat pancreatic extracellular matrix for islet isolation Cell Transplant 6 1997 403
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6. C. Ricordi, D.W.R. Gray, B.J. Hering Islet isolation assessment in man and large animals Acta Diabetol Lat 27 1990 185
7. Brandhorst H, Alt A, Huettler S,, et al. The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas. Transplant Proc (in press)