The effect of truncated collagenase class i isomers on human islet isolation outcome

Transplantation

Volumn 90, Issue 3, 2010, Pages 334-335

  Brandhorst, Heide ^a   Asif, Sana ^a   Andersson, Karin ^a   Mönch, Johanna ^b   Friedrich, Olaf ^c   Rämsch Günther, Nicole ^b   Rämsch, Christian ^c   Steffens, Melanie ^b   Lambrecht, Jörg ^c   Schräder, Thomas ^c   Kurfürst, Manfred ^c   Andersson, Helene H ^d   Felldin, Marie ^e   Foss, Aksel ^f   Salmela, Kaija ^g   Tibell, Annika ^h   Tufveson, Gunnar ⁱ   Korsgren, Olle ^a   Brandhorst, Daniel ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b SERVA Electrophoresis GmbH   (Germany)

^c Nordmark Arzneimittel GmbH and Co KG   (Germany)

^d University Hospital   (Sweden)

^e SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

^f OSLO UNIVERSITY HOSPITAL   (Norway)

^g UNIVERSITY OF HELSINKI   (Finland)

^h KAROLINSKA INSTITUTE   (Sweden)

ⁱ UPPSALA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN BINDING PROTEIN; COLLAGEN FIBER; COLLAGENASE; COLLAGENASE 1; GLUCOSE; INSULIN; NEUTRAL PROTEINASE; PROTEINASE; THERMOLYSIN; UNCLASSIFIED DRUG;

BODY MASS; EMBEDDING; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME METABOLISM; ENZYME PURIFICATION; EXTRACELLULAR MATRIX; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOASSAY; INSULIN RELEASE; ISOLATION AND PURIFICATION; LETTER; OUTCOME ASSESSMENT; PANCREAS; PANCREAS ISLET; PANCREAS ISLET TRANSPLANTATION; PRIORITY JOURNAL;

CLOSTRIDIUM HISTOLYTICUM; HUMANS; ISLETS OF LANGERHANS; ISLETS OF LANGERHANS TRANSPLANTATION; ISOMERISM; MICROBIAL COLLAGENASE; MIDDLE AGED; THERMOLYSIN; TISSUE AND ORGAN HARVESTING; TISSUE CULTURE TECHNIQUES; TISSUE SURVIVAL;

EID: 77955395368     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/TP.0b013e3181e49bd7     Document Type: Letter

References (9)

1. Yamamoto T, Ricordi C, Messinger S, et al. Deterioration and variability of highly purified collagenase blends used in clinical islet isolation. Transplantation 2007; 84: 997.
2. Barnett MJ, Zhai X, LeGatt DF, et al. Quantitative assessment of collagenase blends for human islet isolation. Transplantation 2005; 80: 723.
3. Antonioli B, Fermo I, Cainarca S, et al. Characterization of collagenase blend enzymes for human islet transplantation. Transplantation 2007; 84: 1568.
4. Johnson PR, White SA, London NJ. Collage-nase and human islet isolation. Cell Transplant 1996; 5: 437.
5. McCarthy RC. What is the appropriate assay to assess collagenase prior to use in cell isolation procedures? Available at: http://www. vitacyte.com. Accessed 7 October 2009.
6. Brandhorst H, Brandhorst D, Brendel MD, et al. Assessment of intracellular insulin content during all steps of human islet isolation procedure. Cell Transplant 1998; 7: 489.
7. Brandhorst H, Alt A, Huettler S, et al. The ratio between class II and class I collage-nase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas. Transplant Proc 2005; 37: 215.
8. Brandhorst H, Friberg A, Andersson HH, et al. The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation. Transplantation 2009; 87: 370.
9. Brandhorst H, Raemsch-Guenther N, Raem-sch C, et al. Degraded collagenase deteriorates islet viability. Transplant Proc 2008; 40: 370.