A universal strategy for high-yield production of soluble and functional clostridial collagenases in E. coli

Applied Microbiology and Biotechnology

Volumn 83, Issue 6, 2009, Pages 1055-1065

  Ducka, Paulina ^a   Eckhard, Ulrich ^a   Schönauer, Esther ^a   Kofler, Stefan ^a   Gottschalk, Gerhard ^b   Brandstetter, Hans ^a   Nüss, Dorota ^a  

^a UNIVERSITY OF SALZBURG   (Austria)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Clostridial collagenases; Expression; Platform; Purification

Indexed keywords

BIOCHEMICAL FUNCTIONS; BIOTECHNOLOGICAL TOOLS; CLOSTRIDIAL COLLAGENASES; CLOSTRIDIUM TETANI; COLLAGENASE; COLLAGENOLYSIS; DOWNSTREAM APPLICATIONS; E. COLI; EXPRESSION; EXPRESSION SYSTEM; EXPRESSION VECTORS; IN-VIVO; ISOFORMS; PLATFORM; YIELD PRODUCTION;

CHROMATOGRAPHIC ANALYSIS; COMPETITION; ESCHERICHIA COLI; HAND TOOLS;

PURIFICATION;

COLLAGENASE; COLLAGENASE G; COLLAGENASE H; COLLAGENASE T; ENZYME VARIANT; ISOENZYME; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; CHROMATOGRAPHY; COLIFORM BACTERIUM; COLLAGEN; COLONIZATION; COST-BENEFIT ANALYSIS; ENZYME ACTIVITY; GENE EXPRESSION; PATHOGEN; RECOMBINATION;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CLOSTRIDIUM; CLOSTRIDIUM HISTOLYTICUM; CLOSTRIDIUM TETANI; COLLAGEN DEGRADATION; COST EFFECTIVENESS ANALYSIS; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN MOTIF; QUANTUM YIELD;

BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CLOSTRIDIUM HISTOLYTICUM; CLOSTRIDIUM TETANI; COLLAGENASES; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; RECOMBINANT FUSION PROTEINS;

CLOSTRIDIA; CLOSTRIDIUM HISTOLYTICUM; CLOSTRIDIUM TETANI; ESCHERICHIA COLI;

EID: 67650720738     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-1953-4     Document Type: Article

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