CPAF: A Chlamydial Protease in Search of an Authentic Substrate

PLoS Pathogens

Volumn 8, Issue 8, 2012, Pages

  Chen, Allan L ^a   Johnson, Kirsten A ^a   Lee, Jennifer K ^a   Sütterlin, Christine ^a   Tan, Ming ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN CPAF; PROTEINASE; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ARTICLE; CELL LYSATE; CHLAMYDIA; CYTOSKELETON; ENZYME ACTIVITY; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN DEGRADATION;

APOPTOSIS; BACTERIAL PROTEINS; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; ENDOPEPTIDASES; GOLGI APPARATUS; HELA CELLS; HOST-PATHOGEN INTERACTIONS; HUMANS; PROTEOLYSIS; SUBSTRATE SPECIFICITY;

CHLAMYDIA;

EID: 84866172218     PISSN: 15537366     EISSN: 15537374     Source Type: Journal    
DOI: 10.1371/journal.ppat.1002842     Document Type: Article

References (53)

1. CDC(2011) Summary of notifiable diseases: United States, 2009. MMWR Morb Mortal Wkly Rep 58: 1-100.
2. Schachter J, (1999) Infection and disease epidemiology. In: Stephens RS, editors. Chlamydia: Intracellular Biology, Pathogenesis, and Immunity Washington, D.C. American Society for Microbiology pp. 139-169 editor pp.
3. Burton MJ, Mabey DC, (2009) The global burden of trachoma: a review. PLoS Negl Trop Dis 3: e460.
4. Blasi F, Tarsia P, Aliberti S, (2009) Chlamydophila pneumoniae. Clin Microbiol Infect 15: 29-35.
5. Carabeo RA, Mead DJ, Hackstadt T, (2003) Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion. Proc Natl Acad Sci U S A 100: 6771-6776.
6. Hackstadt T, Rockey D, Heinzen R, Scidmore M, (1996) Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane. EMBO J 15: 964-977.
7. Hackstadt T, Scidmore MA, Rockey DD, (1995) Lipid metabolism in Chlamydia trachomatis-infected cells: directed trafficking of Golgi-derived sphingolipids to the chlamydial inclusion. Proc Natl Acad Sci U S A 92: 4877-4881.
8. Fan T, Lu H, Hu H, Shi L, McClarty GA, et al.(1998) Inhibition of apoptosis in Chlamydia-infected cells: blockade of mitochondrial cytochrome c release and caspase activation. J Exp Med 187: 487-496.
9. Fischer SF, Schwarz C, Vier J, Hacker G, (2001) Characterization of antiapoptotic activities of Chlamydia pneumoniae in human cells. Infect Immun 69: 7121-7129.
10. Rajalingam K, Al-Younes H, Muller A, Meyer TF, Szczepek AJ, et al.(2001) Epithelial cells infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) are resistant to apoptosis. Infect Immun 69: 7880-7888.
11. Zhong G, (2009) Killing me softly: chlamydial use of proteolysis for evading host defenses. Trends Microbiol 17: 467-474.
12. Huang Z, Feng Y, Chen D, Wu X, Huang S, et al.(2008) Structural basis for activation and inhibition of the secreted Chlamydia protease CPAF. Cell Host Microbe 4: 529-542.
13. Dong F, Zhong Y, Arulanandam B, Zhong G, (2005b) Production of a proteolytically active protein, chlamydial protease/proteasome-like activity factor, by five different Chlamydia species. Infect Immun 73: 1868-1872.
14. Horn M, Collingro A, Schmitz-Esser S, Beier CL, Purkhold U, et al.(2004) Illuminating the evolutionary history of chlamydiae. Science 304: 728-730.
15. Zhong G, Fan P, Ji H, Dong F, Huang Y, (2001) Identification of a chlamydial protease-like activity factor responsible for the degradation of host transcription factors. J Exp Med 193: 935-942.
16. Christian JG, Heymann J, Paschen SA, Vier J, Schauenburg L, et al.(2011) Targeting of a chlamydial protease impedes intracellular bacterial growth. PLoS Pathog 7: e1002283.
17. Heuer D, Rejman Lipinski A, Machuy N, Karlas A, Wehrens A, et al.(2009) Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction. Nature 457: 731-735.
18. Paschen SA, Christian JG, Vier J, Schmidt F, Walch A, et al.(2008) Cytopathicity of Chlamydia is largely reproduced by expression of a single chlamydial protease. J Cell Biol 182: 117-127.
19. Pirbhai M, Dong F, Zhong Y, Pan KZ, Zhong G, (2006) The secreted protease factor CPAF is responsible for degrading pro-apoptotic BH3-only proteins in Chlamydia trachomatis-infected cells. J Biol Chem 281: 31495-31501.
20. Kumar Y, Valdivia RH, (2008) Actin and intermediate filaments stabilize the Chlamydia trachomatis vacuole by forming dynamic structural scaffolds. Cell Host Microbe 4: 159-169.
21. Zhong G, Liu L, Fan T, Fan P, Ji H, (2000) Degradation of transcription factor RFX5 during the inhibition of both constitutive and interferon gamma-inducible major histocompatibility complex class I expression in Chlamydia-infected cells. J Exp Med 191: 1525-1534.
22. Christian J, Vier J, Paschen SA, Hacker G, (2010) Cleavage of the NF-kappaB family protein p65/RelA by the chlamydial protease-like activity factor (CPAF) impairs proinflammatory signaling in cells infected with Chlamydiae. J Biol Chem 285: 41320-41327.
23. Sun J, Schoborg RV, (2009) The host adherens junction molecule nectin-1 is degraded by chlamydial protease-like activity factor (CPAF) in Chlamydia trachomatis-infected genital epithelial cells. Microbes Infect 11: 12-19.
24. Belland RJ, Zhong G, Crane DD, Hogan D, Sturdevant D, et al.(2003) Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis. Proc Natl Acad Sci U S A 100: 8478-8483.
25. Holden P, Horton WA, (2009) Crude subcellular fractionation of cultured mammalian cell lines. BMC Res Notes 2: 243.
26. Dick LR, Cruikshank AA, Destree AT, Grenier L, McCormack TA, et al.(1997) Mechanistic studies on the inactivation of the proteasome by lactacystin in cultured cells. J Biol Chem 272: 182-188.
27. Fenteany G, Standaert RF, Lane WS, Choi S, Corey EJ, et al.(1995) Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 268: 726-731.
28. Rajagopalan KV, Fridovich I, Handler P, (1961) Competitive inhibition of enzyme activity by urea. J Biol Chem 236: 1059-1065.
29. Takizawa PA, Yucel JK, Veit B, Faulkner DJ, Deerinck T, et al.(1993) Complete vesiculation of Golgi membranes and inhibition of protein transport by a novel sea sponge metabolite, ilimaquinone. Cell 73: 1079-1090.
30. Dong F, Su H, Huang Y, Zhong Y, Zhong G, (2004c) Cleavage of host keratin 8 by a Chlamydia-secreted protease. Infect Immun 72: 3863-3868.
31. Balsara ZR, Misaghi S, Lafave JN, Starnbach MN, (2006) Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun 74: 5602-5608.
32. Dong F, Pirbhai M, Zhong Y, Zhong G, (2004a) Cleavage-dependent activation of a Chlamydia-secreted protease. Mol Microbiol 52: 1487-1494.
33. Chen D, Chai J, Hart PJ, Zhong G, (2009) Identifying catalytic residues in CPAF, a Chlamydia-secreted protease. Arch Biochem Biophys 485: 16-23.
34. Chen D, Lei L, Flores R, Huang Z, Wu Z, et al.(2010) Autoprocessing and self-activation of the secreted protease CPAF in Chlamydia-infected cells. Microb Pathog 49: 164-173.
35. Dong F, Sharma J, Xiao Y, Zhong Y, Zhong G, (2004b) Intramolecular dimerization is required for the Chlamydia-secreted protease CPAF to degrade host transcriptional factors. Infect Immun 72: 3869-3875.
36. Dong F, Pirbhai M, Xiao Y, Zhong Y, Wu Y, et al.(2005a) Degradation of the proapoptotic proteins Bik, Puma, and Bim with Bcl-2 domain 3 homology in Chlamydia trachomatis-infected cells. Infect Immun 73: 1861-1864.
37. Jorgensen I, Bednar MM, Amin V, Davis BK, Ting JP, et al.(2011) The Chlamydia protease CPAF regulates host and bacterial proteins to maintain pathogen vacuole integrity and promote virulence. Cell Host Microbe 10: 21-32.
38. Lad SP, Li J, da Silva Correia J, Pan Q, Gadwal S, et al.(2007) Cleavage of p65/RelA of the NF-kappaB pathway by Chlamydia. Proc Natl Acad Sci U S A 104: 2933-2938.
39. Rajalingam K, Sharma M, Lohmann C, Oswald M, Thieck O, et al.(2008) Mcl-1 is a key regulator of apoptosis resistance in Chlamydia trachomatis-infected cells. PLoS One 3: e3102.
40. Rajalingam K, Sharma M, Paland N, Hurwitz R, Thieck O, et al.(2006) IAP-IAP complexes required for apoptosis resistance of C. trachomatis-infected cells. PLoS Pathog 2: e114.
41. Fischer U, Janicke RU, Schulze-Osthoff K, (2003) Many cuts to ruin: a comprehensive update of caspase substrates. Cell Death Differ 10: 76-100.
42. Cong Y, Jupelli M, Guentzel MN, Zhong G, Murthy AK, et al.(2007) Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection. Vaccine 25: 3773-3780.
43. Murthy AK, Cong Y, Murphey C, Guentzel MN, Forsthuber TG, et al.(2006) Chlamydial protease-like activity factor induces protective immunity against genital chlamydial infection in transgenic mice that express the human HLA-DR4 allele. Infect Immun 74: 6722-6729.
44. Sharma J, Bosnic AM, Piper JM, Zhong G, (2004) Human antibody responses to a Chlamydia-secreted protease factor. Infect Immun 72: 7164-7171.
45. Skwor T, Kandel RP, Basravi S, Khan A, Sharma B, et al.(2010) Characterization of humoral immune responses to chlamydial HSP60, CPAF, and CT795 in inflammatory and severe trachoma. Invest Ophthalmol Vis Sci 51: 5128-5136.
46. Ossewaarde J, de Vries A, Bestebroer T, Angulo A, (1996) Application of a Mycoplasma group-specific PCR for monitoring decontamination of Mycoplasma-infected Chlamydia sp. strains. Appl Environ Microbiol 62: 328-331.
47. Harlow E, Lane D, (1999) Using antibodies: a laboratory manual Cold Spring Harbor Laboratory Press pp.
48. Fischer SF, Vier J, Kirschnek S, Klos A, Hess S, et al.(2004) Chlamydia inhibit host cell apoptosis by degradation of proapoptotic BH3-only proteins. J Exp Med 200: 905-916.
49. Sun J, Kintner J, Schoborg RV, (2008) The host adherens junction molecule nectin-1 is downregulated in Chlamydia trachomatis-infected genital epithelial cells. Microbiology 154: 1290-1299.
50. Zhong G, Fan T, Liu L, (1999) Chlamydia inhibits interferon gamma-inducible major histocompatibility complex class II expression by degradation of upstream stimulatory factor 1. J Exp Med 189: 1931-1938.
51. Kawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, et al.(2007) CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. J Biol Chem 282: 7368-7375.
52. Yu H, Schwarzer K, Forster M, Kniemeyer O, Forsbach-Birk V, et al.(2010) Role of high-mobility group box 1 protein and poly(ADP-ribose) polymerase 1 degradation in Chlamydia trachomatis-induced cytopathicity. Infect Immun 78: 3288-3297.
53. Rupp J, Gieffers J, Klinger M, van Zandbergen G, Wrase R, et al.(2007) Chlamydia pneumoniae directly interferes with HIF-1alpha stabilization in human host cells. Cell Microbiol 9: 2181-2191.