Performance of three chromogenic VRE screening agars, two Etest® vancomycin protocols, and different microdilution methods in detecting vanB genotype Enterococcus faecium with varying vancomycin MICs

Diagnostic Microbiology and Infectious Disease

Volumn 74, Issue 2, 2012, Pages 171-176

  Klare, Ingo ^a   Fleige, Carola ^a   Geringer, Uta ^a   Witte, Wolfgang ^a   Werner, Guido ^a  

^a ROBERT KOCH INSTITUTE   (Germany)

Author keywords

Antibiotic susceptibility testing; Vancomycin heteroresistance; VRE

Indexed keywords

AGAR; AMPICILLIN; CHLORAMPHENICOL; CIPROFLOXACIN; DALFOPRISTIN PLUS QUINUPRISTIN; DAPTOMYCIN; ERYTHROMYCIN; GENTAMICIN; LINEZOLID; PENICILLIN G; RIFAMPICIN; STREPTOMYCIN; TEICOPLANIN; TETRACYCLINE; TIGECYCLINE; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BROTH DILUTION; CULTURE MEDIUM; ENTEROCOCCUS FAECIUM; EUROPE; GENE FREQUENCY; GENOTYPE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VANA GENE; VANB GENE; VANCOMYCIN RESISTANT ENTEROCOCCUS;

AGAR; BACTERIAL PROTEINS; CHROMOGENIC COMPOUNDS; CULTURE MEDIA; ENTEROCOCCUS FAECIUM; EUROPE; GENOTYPE; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MASS SCREENING; MICROBIAL SENSITIVITY TESTS; SENSITIVITY AND SPECIFICITY; VANCOMYCIN RESISTANCE;

EID: 84866158712     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.06.020     Document Type: Article

References (42)

1. Adler H., Oezcan S., Frei R. Vancomycin-resistant enterococci of vanB genotype may pose problems for screening with highly selective media. J Clin Microbiol 2010, 48:2323.
2. Alam M.R., Donabedian S., Brown W., Gordon J., Chow J.W., Zervos M.J., et al. Heteroresistance to vancomycin in Enterococcus faecium. J Clin Microbiol 2001, 39:3379-3381.
3. Ballard S.A., Pertile K.K., Lim M., Johnson P.D., Grayson M.L. Molecular characterization of vanB elements in naturally occurring gut anaerobes. Antimicrob Agents Chemother 2005, 49:1688-1694.
4. Bourdon N., Berenger R., Lepoultier R., Mouet A., Lesteven C., Borgey F., et al. Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay. Diagn Microbiol Infect Dis 2010, 67:291-293.
5. Bourdon N., Fines-Guyon M., Thiolet J.M., Maugat S., Coignard B., Leclercq R., et al. Changing trends in vancomycin-resistant enterococci in French hospitals, 2001-2008. J Antimicrob Chemother 2011, 66:713-721.
6. Clinical and Laboratory Standards Institute (CLSI) Performance standards for antimicrobial susceptibility testing; Table Enterococcus spp. M02 and M07 (M100-S21). CLSI 2011.
7. Courvalin P. Vancomycin resistance in Gram-positive cocci. Clin Infect Dis 2006, 42(Suppl. 1):S25-S34.
8. B G2 ATP-binding motif, leads to constitutive glycopeptide resistance in VanB-type Enterococcus faecium. Mol Microbiol 2003, 50:1069-1083.
9. Depardieu F., Podglajen I., Leclercq R., Collatz E., Courvalin P. Modes and modulations of antibiotic resistance gene expression. Clin Microbiol Rev 2007, 20:79-114.
10. DIN, Deutsches Institut für Normung DIN 58940, Medical Microbiology - Susceptibility testing of pathogens to antimicrobial agents. Part 8: microdilution. General method specific requirements. DIN Taschenbuch 222: Medizinische Mikrobiologie und Immunologie - Diagnostische Verfahren 2004, 342-355. Beuth-Verlag, Berlin. Deutsches Institut für Normung e.V. (Ed.).
11. Domingo M.C., Huletsky A., Giroux R., Boissinot K., Picard F.J., Lebel P., et al. High prevalence of glycopeptide resistance genes vanB, vanD, and vanG not associated with enterococci in human fecal flora. Antimicrob Agents Chemother 2005, 49:4784-4786.
12. Graham M., Ballard S.A., Grabsch E.A., Johnson P.D.R., Grayson M.L. High rates of fecal carriage of nonenterococcal vanB in both children and adults. Antimicrob Agents Chemother 2008, 52:1195-1197.
13. Gu L., Cao B., Liu Y., Guo P., Song S., Li R., et al. A new Tn1546 type of VanB phenotype-vanA genotype vancomycin-resistant Enterococcus faecium isolates in mainland China. Diagn Microbiol Infect Dis 2009, 63:70-75.
14. Hayden M.K., Trenholme G.M., Schultz J.E., Sahm D.F. In vivo development of teicoplanin resistance in a VanB Enterococcus faecium isolate. J Infect Dis 1993, 167:1224-1227.
15. Henrique P.M., Palazzo I.C., Zanella R.C., Darini A.L. Molecular characterization of enterococci harboring genotype and phenotype incongruence related to glycopeptide resistance isolated in Brazilian hospitals. Mem Inst Oswaldo Cruz 2008, 103:301-305.
16. Jett B., Free L., Sahm D.F. Factors influencing the Vitek Gram-positive susceptibility system's detection of vanB-encoded vancomycin resistance among enterococci. J Clin Microbiol 1996, 34:701-706.
17. Kawalec M., Gniadkowski M., Kedzierska J., Skotnicki A., Fiett J., Hryniewicz W. Selection of a teicoplanin-resistant Enterococcus faecium mutant during an outbreak caused by vancomycin-resistant enterococci with the vanB phenotype. J Clin Microbiol 2001, 39:4274-4282.
18. Khan S.A., Sung K., Layton S., Nawaz M.S. Heteroresistance to vancomycin and novel point mutations in Tn1546 of Enterococcus faecium ATCC 51559. Int J Antimicrob Agents 2008, 31:27-36.
19. Klare I., Heier H., Claus H., Böhme G., Marin S., Seltmann G., et al. Enterococcus faecium strains with vanA-mediated high-level glycopeptide resistance isolated from animal foodstuffs and fecal samples of humans in the community. Microb Drug Resist 1995, 1:265-272.
20. Klare I., Heier H., Claus H., Reissbrodt R., Witte W. vanA-mediated high-level glycopeptide resistance in Enterococcus faecium from animal husbandry. FEMS Microbiol Lett 1995, 125:165-171.
21. Klare I., Badstubner D., Konstabel C., Böhme G., Claus H., Witte W. Decreased incidence of VanA-type vancomycin-resistant enterococci isolated from poultry meat and from fecal samples of humans in the community after discontinuation of avoparcin usage in animal husbandry. Microb Drug Resist 1999, 5:45-52.
22. Klare I., Konstabel C., Mueller-Bertling S., Werner G., Strommenger B., Kettlitz C., et al. Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals. Eur J Clin Microbiol Inf Dis 2005, 24:815-825.
23. Klare I., Werner G., Witte W. Enterococci with vancomycin resistance from German hospitals in 2008/2009 [German]. Epidemiol Bull 2010, 44:427-437.
24. Leavis H.L., Willems R.J.L., Top J., Bonten M.J.M. High-Level ciprofloxacin resistance from point mutations in gyrA and parC confined to global hospital-adapted clonal lineage CC17 of Enterococcus faecium. J Clin Microbiol 2006, 44:1059-1064.
25. Lefort A., Arthur M., Depardieu F., Chau F., Pouzet C., Courvalin P., Fantin B. Expression of glycopeptide-resistance gene in response to vancomycin and teicoplanin in the cardiac vegetations of rabbits infected with VanB-type Enterococcus faecalis. J Infect Dis 2004, 189:90-97.
26. Park I.J., Lee W.G., Shin J.H., Lee K.W., Woo G.J. VanB phenotype-vanA genotype Enterococcus faecium with heterogeneous expression of teicoplanin resistance. J Clin Microbiol 2008, 46:3091-3093.
27. Qu T.T., Zhang J.L., Zhou Z.H., Wei Z.Q., Yu Y.S., Chen Y.G., et al. Heteroresistance to teicoplanin in Enterococcus faecium harboring the vanA gene. J Clin Microbiol 2009, 47:4194-4196.
28. Raponi G., Ghezzi M.C., Gherardi G., Lorino G., Dicuonzo G. Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates. J Med Microbiol 2010, 59:672-678.
29. Rathe M., Kristensen L., Ellermann-Eriksen S., Thomsen M.K., Schumacher H. Vancomycin-resistant Enterococcus spp.: validation of susceptibility testing and in vitro activity of vancomycin, linezolid, tigecycline and daptomycin. APMIS 2010, 118:66-73.
30. San Millan A., Depardieu F., Godreuil S., Courvalin P. VanB-type Enterococcus faecium clinical isolate successively inducibly resistant to, dependent on, and constitutively resistant to vancomycin. Antimicrob Agents Chemother 2009, 53:1974-1982.
31. Soderblom T., Aspevall O., Erntell M., Hedin G., Heimer D., Hokeberg I., et al. Alarming spread of vancomycin resistant enterococci in Sweden since 2007. Euro Surveill 2010, 15:19620.
32. Song J.H., Ko K.S., Oh W.S., Park S., Heo S.T., Kwon K.T., et al. High frequency of vancomycin-resistant Enterococcus faecium isolates with VanB phenotype and vanA genotype in Korean hospitals. Diagn Microbiol Inf Dis 2006, 56:401-406.
33. Stamper P.D., Cai M., Lema C., Eskey K., Carroll K.C. Comparison of the BD GeneOhm VanR Assay to culture for identification of vancomycin-resistant enterococci in rectal and stool specimens. J Clin Microbiol 2007, 45:3360-3365.
34. Tenover F.C., Swenson J.M., O'Hara C.M., Stocker S.A. Ability of commercial and reference antimicrobial susceptibility testing methods to detect vancomycin resistance in enterococci. J Clin Microbiol 1995, 33:1524-1527.
35. Usacheva E.A., Ginocchio C.C., Morgan M., Maglanoc G., Mehta M.S., Tremblay S., et al. Prospective, multicenter evaluation of the BD GeneOhm VanR assay for direct, rapid detection of vancomycin-resistant Enterococcus species in perianal and rectal specimens. Am J Clin Pathol 2010, 134:219-226.
36. Werner G., Klare I., Fleige C., Witte W. Increasing rates of vancomycin resistance among Enterococcus faecium isolated from German hospitals between 2004 and 2006 are due to wide clonal dissemination of vancomycin-resistant enterococci and horizontal spread of vanA clusters. Int J Med Microbiol 2007, 298:515-527.
37. Werner G., Strommenger B., Witte W. Acquired vancomycin resistance in clinically relevant pathogens. Future Microbiol 2008, 3:547-562.
38. Werner G., Fleige C., Ewert B., Laverde-Gomez J.A., Klare I., Witte W. High-level ciprofloxacin resistance among hospital-adapted Enterococcus faecium (CC17). Int J Antimicrob Agents 2010, 35:119-125.
39. Werner G., Fleige C., Geringer U., van Schaik W., Klare I., Witte W. IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium. BMC Infect Dis 2011, 11:e80.
40. Werner G., Serr A., Schütt S., Schneider C., Klare I., Witte W., et al. Comparison of direct cultivation on a selective solid medium, polymerase chain reaction from an enrichment broth, and the BD GeneOhmTM VanR Assay for identification of vancomycin-resistant enterococci in screening specimens. Diagn Microbiol Inf Dis 2011, 70:512-521.
41. Werner G. Surveillance of antimicrobial resistance among Enterococcus faecium and Enterococcus faecalis isolated from human (clinical/commensal), food animal, meat and environmental samples. In: Semedo-Lemsaddek T, Barreto-Crespo MT, Tenreiro R (Eds), Enterococcus and safety. 2012. Nova Science Publishers Inc., Hauppage, NY.
42. Young H.L., Ballard S.A., Roffey P., Grayson M.L. Direct detection of vanB2 using the Roche LightCycler vanA/B detection assay to indicate vancomycin-resistant enterococcal carriage - sensitive but not specific. J Antimicrob Chemother 2007, 59:809-810.