Prospective, multicenter evaluation of the BD GeneOhm VanR assay for direct, rapid detection of vancomycin-resistant Enterococcus species in perianal and rectal specimens

American Journal of Clinical Pathology

Volumn 134, Issue 2, 2010, Pages 219-226

  Usacheva, Elena A ^a   Ginocchio, Christine C ^b   Morgan, Margie ^c   Maglanoc, Geronimo ^c   Mehta, Maitry S ^a   Tremblay, Sebastien ^d   Karchmer, Tobi B ^e   Peterson, Lance R ^a  

^a NORTHSHORE UNIVERSITY HEALTHSYSTEM   (United States)

^b North Shore LIJ Health System Laboratories ^*  (United States)

^c CEDARS SINAI MEDICAL CENTER   (United States)

^d Infectious Disease   (Canada)

^e Infectious Disease Laboratory   (United States)

Author keywords

Infection control; Real time polymerase chain reaction; Surveillance; Vancomycin resistant enterococci; VRE

Indexed keywords

NUCLEIC ACID; VANCOMYCIN;

ARTICLE; ASSAY; BACTERIUM CULTURE; BD GENEOHM VANR ASSAY; DIAGNOSTIC TEST; FALSE POSITIVE RESULT; GENOTYPE; HUMAN; MAJOR CLINICAL STUDY; METHODOLOGY; NONHUMAN; PERIANAL CULTURE; POLYMERASE CHAIN REACTION; POPULATION; PREDICTIVE VALIDITY; PREVALENCE; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUALITATIVE ANALYSIS; RECTUM CULTURE; SCREENING; SENSITIVITY AND SPECIFICITY; SPECIES; UNITED STATES; VANCOMYCIN RESISTANT ENTEROCOCCUS;

ANAL CANAL; BACTERIAL PROTEINS; CARBON-OXYGEN LIGASES; DNA, BACTERIAL; ENTEROCOCCUS; FALSE POSITIVE REACTIONS; FEMALE; HUMANS; MALE; POLYMERASE CHAIN REACTION; PREVALENCE; REAGENT KITS, DIAGNOSTIC; RECTUM; SENSITIVITY AND SPECIFICITY; VANCOMYCIN RESISTANCE;

EID: 77955592392     PISSN: 00029173     EISSN: 19437722     Source Type: Journal    
DOI: 10.1309/AJCPR1K0QFLBJSNH     Document Type: Article

References (30)

1. Centers for Disease Control and Prevention. Recommendations for preventing the spread of vancomycin resistance: recommendations of the Hospital Infection Control Practices Advisory Committee (HICPAC). MMWR Recomm Rep. 1995;44(RR-12):1-13.
2. Weigel LM, Clewell DB, Gill SR, et al. Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science. 2003;302:1569-1571.
3. Ferber D. Microbiology: triple-threat microbe gained powers from another bug. Science. 2003;302:1488. (Pubitemid 37475667)
4. Rice LB. Emergence of vancomycin-resistant enterococci. Emerg Infect Dis. 2001;7:183-187.
5. Carmeli Y, Eliopoulos G, Mozaffari E, et al. Health and economic outcomes of vancomycin-resistant enterococci. Arch Intern Med. 2002;162:2223-2228. (Pubitemid 35231592)
6. Ratanasuwan W, Iwen PC, Hinrichs SH, et al. Bacteremia due to motile Enterococcus species: clinical features and outcomes. Clin Infect Dis. 1999;28:1175-1177. (Pubitemid 29203569)
7. Facklam RR, Sahm DF, Teixeira LM. Enterococcus. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 7th ed. Washington, DC: American Society for Microbiology; 1999:297-305.
8. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. CLSI document M100-S17.
9. Domingo MC, Huletsky A, Giroux R, et al. High prevalence of glycopeptide resistance genes vanB, vanD, and vanG not associated with enterococci in human fecal flora. Antimicrob Agents Chemother. 2005;49:4784-4786. (Pubitemid 41552619)
10. Ke D, Menard C, Picard FJ, et al. Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clin Chem. 2000;46:324-331. (Pubitemid 30152257)
11. Sood S, Malhotra M, Das BK, et al. Enterococcal infections & antimicrobial resistance. Indian J Med Res. 2008;128:111-121.
12. Werner G, Coque TM, Hammerum AM, et al. Emergence and spread of vancomycin resistance among enterococci in Europe. Euro Surveill. 2008;13:pii 19046.
13. Willems RJ, Top J, van Santen M, et al. Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis. 2005;11:821-828. (Pubitemid 40720841)
14. Schmidt-Hieber M, Blau IW, Schwartz S, et al. Intensified strategies to control vancomycin-resistant enterococci in immunocompromised patients. Int J Hematol. 2007;86:158-162. (Pubitemid 47425195)
15. Linden PK, Pasculle AW, Manez R, et al. Differences in outcomes for patients with bacteremia due to vancomycin-resistant Enterococcus faecium or vancomycin-susceptible E. faecium. Clin Infect Dis. 1996;22:663-670. (Pubitemid 26286313)
16. Lodise TP, McKinnon PS, Tam VH, et al. Clinical outcomes for patients with bacteremia caused by vancomycin-resistant Enterococcus in a level 1 trauma center. Clin Infect Dis. 2002;34:922-929.
17. Price CS, Paule S, Noskin GA, et al. Active surveillance reduces the incidence of vancomycin-resistant enterococcal bacteremia. Clin Infect Dis. 2003;37:921-928. (Pubitemid 37267206)
18. Lee TA, Hacek DM, Stroupe KT, et al. Three surveillance strategies for vancomycin-resistant enterococci in hospitalized patients: detection of colonization efficiency and a cost-effectiveness model. Infect Control Hosp Epidemiol. 2005;26:39-46.
19. Montecalvo MA, Jarvis WR, Uman J, et al. Costs and savings associated with infection control measures that reduced transmission of vancomycin-resistant enterococci in an endemic setting. Infect Control Hosp Epidemiol. 2001;22:437-442. (Pubitemid 32889276)
20. Stamper PD, Cai M, Lema C, et al. Comparison of the BD GeneOhm VanR assay to culture for identification of vancomycin-resistant enterococci in rectal and stool specimens. J Clin Microbiol. 2007;45:3360-3365. (Pubitemid 47585662)
21. Paule SM, Trick WE, Tenover FC, et al. Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci. J Clin Microbiol. 2003;41:4805-4807. (Pubitemid 37259800)
22. McGee S. Simplifying likelihood ratios. J Gen Intern Med. 2002;17:646-649.
23. Mehta MS, Paule SM, Hacek DM, et al. Optimization of a laboratory-developed test utilizing Roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species. J Clin Microbiol. 2008;46:2377-2380.
24. Stinear TP, Olden DC, Johnson PD, et al. Enterococcal vanB resistance locus in anaerobic bacteria in human faeces. Lancet. 2001;357:855-856.
25. Ballard SA, Grabsch EA, Johnson PD, et al. Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli. Antimicrob Agents Chemother. 2005;49:77-81.
26. Dahl KH, Sundsfjord A. Transferable vanB2 Tn5382-containing elements in fecal streptococcal strains from veal calves. Antimicrob Agents Chemother. 2003;47:2579-2583. (Pubitemid 36919462)
27. Sievert DM, Rudrik JT, Patel JB, et al. Vancomycin-resistant Staphylococcus aureus in the United States, 2002-2006. Clin Infect Dis. 2008;46:668-674.
28. Ligozzi M, Lo Cascio G, Fontana R. vanA gene cluster in a vancomycin-resistant clinical isolate of Bacillus circulans. Antimicrob Agents Chemother. 1998;42:2055-2059.
29. Power EG, Abdulla YH, Talsania HG, et al. vanA genes in vancomycin-resistant clinical isolates of Oerskovia turbata and Arcanobacterium (Corynebacterium) haemolyticum. J Antimicrob Chemother. 1995;36:595-606.
30. Altman DG, Bland JM. Diagnostic tests 2: predictive values. BMJ. 1994;309:102.