Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system

Journal of Lipid Research

Volumn 53, Issue 10, 2012, Pages 2242-2251

  Ishibashi, Yohei ^a,d   Kobayashi, Utaro ^a   Hijikata, Atsushi ^b   Sakaguchi, Keishi ^a   Goda, Hatsumi M ^a   Tamura, Tomohiro ^c   Okino, Nozomu ^a   Ito, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b RIKEN Center for Integrative Medical Sciences   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^d RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

Author keywords

Endoglycoceramidase; Flexible loop; Globo series glycosphingolipids; Glycosphingolipid; Oligosaccharide; Rhodococcus

Indexed keywords

BACTERIAL ENZYME; CERAMIDASE; ENDOGLYCOCERAMIDASE I; ENDOGLYCOCERAMIDASE II; GLYCOSPHINGOLIPID; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME METABOLISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENE EXPRESSION SYSTEM; LIPID ANALYSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; RHODOCOCCUS ERYTHROPOLIS; THREE DIMENSIONAL IMAGING;

CATALYSIS; CLONING, MOLECULAR; GLYCOSIDE HYDROLASES; GLYCOSPHINGOLIPIDS; KINETICS; RHODOCOCCUS;

EID: 84866155016     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D028951     Document Type: Article

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