A Gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5432-5434

  Nakayama, Yoshitaka ^a   Yoshimura, Kenjiro ^b   Iida, Hidetoshi ^a  

^a TOKYO GAKUGEI UNIVERSITY   (Japan)

^b Bldg 142   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CORYNEBACTERIUM GLUTAMICUM;

AMINO ACID TRANSPORTER; BACTERIAL PROTEIN; GLUTAMIC ACID;

AMINO ACID SEQUENCE; ARTICLE; CHANNEL GATING; CORYNEBACTERIUM GLUTAMICUM; GENETICS; METABOLISM; MISSENSE MUTATION; MOLECULAR GENETICS; PATCH CLAMP; SECRETION (PROCESS); SPECIES DIFFERENCE;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEMS; BACTERIAL PROTEINS; CORYNEBACTERIUM GLUTAMICUM; GLUTAMIC ACID; ION CHANNEL GATING; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; PATCH-CLAMP TECHNIQUES; SPECIES SPECIFICITY;

CORYNEBACTERIUM GLUTAMICUM; MECHANOSENSITIVE CHANNEL; PATCH CLAMP EXPERIMENTS; WILD TYPES;

BIOTECHNOLOGY; MICROBIOLOGY;

ELECTROPHYSIOLOGY;

BACTERIUM; EXPERIMENTAL STUDY; MUTATION; SECRETION; THRESHOLD;

EID: 84866145083     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01310-12     Document Type: Note

References (14)

1. Bass RB, Strop P, Barclay M, Rees DC. 2002. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science 298:1582-1587.
2. Börngen K, et al. 2010. The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation. Biochim. Biophys. Acta 1798:2141-2149.
3. Edwards MD, et al. 2005. Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel. Nat. Struct. Mol. Biol. 12:113-119.
4. Hashimoto K, et al. 2010. The protein encoded by NCgl1221 in Corynebacterium glutamicum functions as a mechanosensitive channel. Biosci. Biotechnol. Biochem. 74:2546-2549.
5. Kim J, Hirasawa T, Saito M, Furusawa C, Shimizu H. 2011. Investigation of phosphorylation status of OdhI protein during penicillin- and Tween 40-triggered glutamate overproduction by Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 91:143-151.
6. Li Y, Moe PC, Chandrasekaran S, Booth IR, Blount P. 2002. Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli. EMBO J. 21:5323-5330.
7. Martinac B, Buechner M, Delcour AH, Adler J, Kung C. 1987. Pressuresensitive ion channel in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 84:2297-2301.
8. Nakamura J, Hirano S, Ito H, Wachi M. 2007. Mutations of the Corynebacterium glutamicum NCgl1221 gene, encoding a mechanosensitive channel homolog, induce L-glutamic acid production. Appl. Environ. Microbiol. 73:4491-4498.
9. Okada K, Moe PC, Blount P. 2002. Functional design of bacterial mechanosensitive channels. J. Biol. Chem. 277:27682-27688.
10. Schumann U, et al. 2010. YbdG in Escherichia coli is a threshold-setting mechanosensitive channel with MscM activity. Proc. Natl. Acad. Sci. U. S. A. 107:12664-12669.
11. Shiio I, Otsuka SI, Takahashi M. 1962. Effect of biotin on the bacterial formation of glutamic acid. I. Glutamate formation and cellular permeability of amino acids. J. Biochem. 51:56-62.
12. Sukharev S. 2002. Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. Biophys. J. 83:290-298.
13. Wang W, et al. 2008. The structure of an open form of an E. coli mechanosensitive channel at 3.45åresolution. Science 321:1179-1183.
14. Yao W, et al. 2009. Expression and localization of the Corynebacterium glutamicum NCgl1221 protein encoding an L-glutamic acid exporter. Microbiol. Res. 164:680-687.