SUMO fusion system facilitates soluble expression and high production of bioactive human fibroblast growth factor 23 (FGF23)

Applied Microbiology and Biotechnology

Volumn 96, Issue 1, 2012, Pages 103-111

  Liu, Xiaoju ^a,c,d   Chen, Yubin ^c   Wu, Xiaoping ^d   Li, Haiyan ^c   Jiang, Chao ^a,c,d   Tian, Haishan ^c,d   Tang, Lu ^d   Wang, Dezhong ^c,d   Yu, Ting ^a,b   Li, Xiaokun ^a,c,d  

^a JILIN UNIVERSITY   (China)

^b THE SECOND HOSPITAL OF JILIN UNIVERSITY   (China)

^c BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^d WENZHOU MEDICAL UNIVERSITY   (China)

Author keywords

Bioactive fibroblast growth factor 23 (FGF23); High production; Soluble expression; SUMO fusion

Indexed keywords

AFTER-TREATMENT; ANIMAL EXPERIMENTS; ESCHERICHIA COLI ROSETTA; FERMENTORS; FIBROBLAST GROWTH FACTOR-23; FUSION GENES; FUSION PROTEINS; FUSION SYSTEMS; GALACTOPYRANOSIDE; HOST STRAIN; HUMAN DISEASE; HUMAN FIBROBLAST; IN-VITRO; IN-VIVO; MITOGEN ACTIVATED PROTEIN KINASE; NITRILOTRIACETIC ACID; PCR METHOD; ROSETTA; SMALL UBIQUITIN-RELATED MODIFIERS; SOLUBLE EXPRESSION; THERAPEUTIC TARGETS;

AFFINITY CHROMATOGRAPHY; ESCHERICHIA COLI; GENES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; PURIFICATION;

CLONING;

ANTINEOPLASTIC AGENT; MITOGEN ACTIVATED PROTEIN KINASE; RECOMBINANT FIBROBLAST GROWTH FACTOR; SMALL UBIQUITIN RELATED MODIFIER RECOMBINANT FIBROBLAST GROWTH FACTOR 23; UNCLASSIFIED DRUG;

BIOACTIVITY; BIOCHEMISTRY; BIOLOGICAL PRODUCTION; CYTOLOGY; DIET; DISEASE TREATMENT; ENZYME ACTIVITY; EXPERIMENTAL STUDY; GENE EXPRESSION; GROWTH RATE; HOMEOSTASIS; MEDICINE; NICKEL; PATHOGENICITY; PHOSPHATE; PLASMA; PLASMID; POLYMERASE CHAIN REACTION; PUBLIC HEALTH;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL STRAIN; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME PHOSPHORYLATION; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE FUSION; GLIOMA CELL; HUMAN; LIQUID CHROMATOGRAPHY; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RAT;

BIOTECHNOLOGY; CELL LINE, TUMOR; CLONING, MOLECULAR; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTORS; GENE EXPRESSION; GENETIC VECTORS; HUMANS; MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; PLASMIDS; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SUMOYLATION; TECHNOLOGY, PHARMACEUTICAL; TRANSFORMATION, BACTERIAL;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; RATTUS;

EID: 84866043459     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3864-4     Document Type: Article

References (20)

1. Butt TR, Edavettal S, Hall JP, Mattern MR (2005) SUMO fusion technology for difficult-to-express proteins. Protein Expr Purif 43:1-9
2. Fliser D, Kollerits B, Neyer U, Ankerst DP, Lhotta K, Lingenhel A, Ritz E, Kronenberg F, Kuen E, Konig P, Kraatz G, Mann JF, Muller GA, Kohler H, Riegler P (2007) Fibroblast growth factor 23 (FGF23) predicts progression of chronic kidney disease: the Mild to Moderate Kidney Disease (MMKD) study. J Am Soc Nephrol 18:2600-2608
3. Goetz R, Beenken A, Ibrahimi OA, Kalinina J, Olsen SK, Eliseenkova AV, Xu C, Neubert TA, Zhang F, Linhardt RJ, Yu X, White KE, Inagaki T, Kliewer SA, Yamamoto M, Kurosu H, Ogawa Y, Kuro-o M, Lanske B, Razzaque MS, Mohammadi M (2007) Molecular insights into the klotho-dependent, endocrine mode of action of fibroblast growth factor 19 subfamily members. Mol Cell Biol 27:3417-3428
4. Gutierrez O, Isakova T, Rhee E, Shah A, Holmes J, Collerone G, Juppner H, Wolf M (2005) Fibroblast growth factor-23 mitigates hyperphosphatemia but accentuates calcitriol deficiency in chronic kidney disease. J Am Soc Nephrol 16:2205-2215
5. Huang Y, Su Z, Li Y, Zhang Q, Cui L, Su Y, Ding C, Zhang M, Feng C, Tan Y, Feng W, Li X, Cai L (2009) Expression and purification of glutathione transferase-small ubiquitin-related modifier-metallothionein fusion protein and its neuronal and hepatic protection against d-galactose-induced oxidative damage in mouse model. J Pharmacol Exp Ther 329:469-478
6. Jonsson KB, Zahradnik R, Larsson T, White KE, Sugimoto T, Imanishi Y, Yamamoto T, Hampson G, Koshiyama H, Ljunggren O, Oba K, Yang IM, Miyauchi A, Econs MJ, Lavigne J, Juppner H (2003) Fibroblast growth factor 23 in oncogenic osteomalacia and X-linked hypophosphatemia. N Engl J Med 348:1656-1663
7. Juppner H, Wolf M, Salusky IB (2010) FGF-23: More than a regulator of renal phospate handing? J Bone Miner Res 25:2091-2097
8. Lyles KW, Burkes EJ, Ellis GJ, Lucas KJ, Dolan EA, Drezner MK (1985) Genetic transmission of tumoral calcinosis: autosomal dominant with variable clinical expressivity. J Clin Endocrinol Metab 60:1093-1096
9. Malakhov MP, Mattern MR, Malakhova OA, Drinker M, Weeks SD, Butt TR (2004) SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. J Struct Funct Genomics 5:75-86
10. Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, Butt TR (2006) Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO. Protein Sci 15:182-189
11. Panavas T, Sanders C, Butt TR (2009) SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems. Methods Mol Biol 497:303-317
12. Plotnikov AN, Hubbard SR, Schlessinger J, Mohammadi M (2000) Crystal structures of two FGF-FGFR complexes reveal the determinants of ligand-receptor specificity. Cell 101:413-424
13. Riminucci M, Collins MT, Fedarko NS, Cherman N, Corsi A, White KE, Waguespack S, Gupta A, Hannon T, Econs MJ, Bianco P, Gehron Robey P (2003) FGF-23 in fibrous dysplasia of bone and its relationship to renal phosphate wasting. J Clin Invest 112:683-692
14. Shimada T, Hasegawa H, Yamazaki Y, Muto T, Hino R, Takeuchi Y, Fujita T, Nakahara K, Fukumoto S, Yamashita T (2004) FGF-23 is a potent regulator of vitamin D metabolism and phosphate homeostasis. J Bone Miner Res 19:429-435
15. Sun Z, Xia Z, Bi F, Liu JN (2008) Expression and purification of human urodilatin by small ubiquitin-related modifier fusion in Escherichia coli. Appl Microbiol Biotechnol 78:495-502
16. Wang H, Xiao Y, Fu L, Zhao H, Zhang Y, Wan X, Qin Y, Huang Y, Gao H, Li X (2010) High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli. BMC Biotechnol 10:14
17. Wu X, Nie C, Huang Z, Nie Y, Yan Q, Xiao Y, Su Z, Huang Y, Xiao J, Zeng Y, Tan Y, Feng W, Li X (2009) Expression and purification of human keratinocyte growth factor 2 by fusion with SUMO. Mol Biotechnol 42:68-74
18. Yamashita T, Yoshioka M, Itoh N (2000) Identification of a novel fibroblast growth factor, FGF-23, preferentially expressed in the ventrolateral thalamic nucleus of the brain. Biochem Biophys Res Commun 277:494-498
19. Yamazaki Y, Okazaki R, Shibata M, Hasegawa Y, Satoh K, Tajima T, Takeuchi Y, Fujita T, Nakahara K, Yamashita T, Fukumoto S (2002) Increased circulatory level of biologically active full-length FGF-23 in patients with hypophosphatemic rickets/osteomalacia. J Clin Endocrinol Metab 87:4957-4960
20. Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR (2005) Expression and purification of SARS coronavirus proteins using SUMO-fusions. Protein Expr Purif 42:100-110