Expression and purification of human keratinocyte growth factor 2 by fusion with SUMO

Molecular Biotechnology

Volumn 42, Issue 1, 2009, Pages 68-74

  Wu, Xiaoping ^a   Nie, Changjun ^a,b   Huang, Zhifeng ^a,b   Nie, Yanfang ^a   Yan, Qiuxia ^a   Xiao, Yecheng ^c   Su, Zhijian ^a   Huang, Yadong ^a   Xiao, Jian ^b   Zeng, Yaoying ^a   Tan, Yi ^b   Feng, Wenke ^b,d   Li, Xiaokun ^a,b,c  

^a JINAN UNIVERSITY   (China)

^b WENZHOU MEDICAL UNIVERSITY   (China)

^c JILIN AGRICULTURAL UNIVERSITY   (China)

^d University of Louisville School of Medicine   (United States)

Author keywords

Expression and purification; Fusion protein; Human keratinocyte growth factor 2; Mitogenic activity; Small ubiquitin related modifier

Indexed keywords

EXPRESSION AND PURIFICATION; FUSION PROTEIN; HUMAN KERATINOCYTE GROWTH FACTOR 2; MITOGENIC ACTIVITY; SMALL UBIQUITIN-RELATED MODIFIER;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; DESALINATION; ESCHERICHIA COLI; ION EXCHANGE; NUCLEIC ACIDS; PEPTIDES; PURIFICATION;

SIZE EXCLUSION CHROMATOGRAPHY;

CELL PROTEIN; DNA FRAGMENT; FIBROBLAST GROWTH FACTOR 10; HEXAHISTIDINE; HYBRID PROTEIN; SEPHADEX; SUMO PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CELL PROLIFERATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE; GENE AMPLIFICATION; GENE CONSTRUCT; GENE FUSION; GENE INSERTION; HUMAN; KIDNEY EPITHELIUM; MITOGENICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; RAT; RECOMBINANT PLASMID; ANIMAL; CELL LINE; DRUG EFFECT; GENETICS; METABOLISM; TEMPERATURE;

ESCHERICHIA COLI; RATTUS;

ANIMALS; CELL LINE; CELL PROLIFERATION; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTOR 10; HUMANS; RATS; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; TEMPERATURE;

EID: 69149108164     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9135-7     Document Type: Article

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