Verification of complete bisulfite modification using Calponin-specific primer sets

Clinical Biochemistry

Volumn 43, Issue 4-5, 2010, Pages 528-530

  Sriraksa, Ruethairat ^a   Chaopatchayakul, Patimaporn ^a   Jearanaikoon, Patcharee ^a   Leelayuwat, Chanvit ^a   Limpaiboon, Temduang ^a  

^a KHON KAEN UNIVERSITY   (Thailand)

Author keywords

Bisulfite modification; Calponin specific primers; DNA methylation; Epigenetics; Methylation specific PCR

Indexed keywords

BISULFITE; CALPONIN; DNA; STRATIFIN;

ALLELE; ARTICLE; CONTROLLED STUDY; CPG ISLAND; DNA METHYLATION; DNA MODIFICATION; DNA SCREENING; FALSE POSITIVE RESULT; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WILD TYPE;

CALCIUM-BINDING PROTEINS; DNA METHYLATION; DNA PRIMERS; HUMANS; MICROFILAMENT PROTEINS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SULFITES;

EID: 76749154991     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2009.11.005     Document Type: Article

References (10)

1. Baylin S.B. DNA methylation and gene silencing in cancer. Nat. Clin. Pract. Oncol. 2 Suppl. 1 (2005) S4-S11
2. Esteller M. Epigenetics provides a new generation of oncogenes and tumour-suppressor genes. Br. J. Cancer 94 (2006) 179-183
3. Lyko F., and Brown R. DNA methyltransferase inhibitors and the development of epigenetic cancer therapies. J. Natl. Cancer Inst. 97 (2005) 1498-1506
4. Clark S.J., Statham A., Stirzaker C., Molloy P.L., and Frommer M. DNA methylation: bisulphite modification and analysis. Nat. Protoc. 1 (2006) 2353-2364
5. Wang R.Y., Gehrke C.W., and Ehrlich M. Comparison of bisulfite modification of 5-methyldeoxycytidine and deoxycytidine residues. Nucleic Acids Res. 8 (1980) 4777-4790
6. Rand K., Qu W., Ho T., Clark S.J., and Molloy P. Conversion-specific detection of DNA methylation using real-time polymerase chain reaction (ConLight-MSP) to avoid false positives. Methods 27 (2002) 114-120
7. Gusev N.B. Some properties of caldesmon and calponin and the participation of these proteins in regulation of smooth muscle contraction and cytoskeleton formation. Biochemistry (Mosc.) 66 (2001) 1112-1121
8. Herman J.G., Graff J.R., Myohanen S., Nelkin B.D., and Baylin S.B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 9821-9826
9. Teodoridis J.M., Strathdee G., and Brown R. Epigenetic silencing mediated by CpG island methylation: potential as a therapeutic target and as a biomarker. Drug Resist. Updat. 7 (2004) 267-278
10. Lee S., Kim W.H., Jung H.Y., Yang M.H., and Kang G.H. Aberrant CpG island methylation of multiple genes in intrahepatic cholangiocarcinoma. Am. J. Pathol. 161 (2002) 1015-1022