Basic vapor exposure for tuning the charge state distribution of proteins in negative electrospray ionization: Elucidation of mechanisms by fluorescence spectroscopy

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 7, 2012, Pages 1221-1231

  Girod, Marion ^a   Antoine, Rodolphe ^a   Dugourd, Philippe ^a   Love, Craig ^a,b   Mordehai, Alex ^a,b   Stafford, George ^a,b  

^a UNIVERSITÉ LYON 1   (France)

^b AGILENT TECHNOLOGIES   (United States)

Author keywords

Charge state distributions; Electrospray ionization; Fluorescence spectroscopy; Mechanisms; PH; Protein

Indexed keywords

AGILENT; AGILENT TECHNOLOGIES; CHARGE STATE DISTRIBUTION; DROPLET EVAPORATION; ELECTRICAL CONDUCTIVITY; ELECTROSPRAY PLUME; INITIAL SOLUTION; ION INTENSITIES; JET STREAMS; LASER INDUCED FLUORESCENCE; MASS SPECTRA; OPTICAL SET-UP; PROTEIN CHARGE; SANTA CLARA; SHEATH GAS; SOLUTION CONDITIONS; SOURCE GEOMETRY; SPRAY CURRENT;

DROPS; ELECTRIC CONDUCTIVITY; ELECTRON ENERGY LEVELS; ELECTROSPRAY IONIZATION; FLUORESCENCE SPECTROSCOPY; MASS SPECTROMETRY; MECHANISMS; NITROGEN; PH; PH EFFECTS; PROTEINS;

VAPORS;

AMINE; HYDROXYL RADICAL; NITROGEN; SOLVENT; WATER;

ARTICLE; CORRELATION ANALYSIS; ELECTRIC CONDUCTIVITY; ELECTROLYSIS; ELECTROSPRAY MASS SPECTROMETRY; EVAPORATION; FLUORESCENCE ANALYSIS; FLUORESCENCE SPECTROSCOPY; LIMIT OF DETECTION; NEBULIZER; PH; PLUME; PROTEIN ANALYSIS; SURFACE CHARGE; VAPOR;

EQUIPMENT DESIGN; GASES; HYDROGEN-ION CONCENTRATION; PROTEINS; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84865859254     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-012-0375-1     Document Type: Article

References (64)

1. Fenn, J.B., Mann, M., Meng, C.K., Wong, S.F., Whitehouse, C.M.: Electrospray ionization for mass spectrometry of large biomolecules. Science 246, 64 (1989)
2. Mann, M., Meng, C.K., Fenn, J.B.: Interpreting mass spectra of multiply charged ions. Anal. Chem. 61, 1702-1708 (1989)
3. Chowdhury, S.K., Katta, V., Chait, B.T.: Probing conformational changes in proteins by mass spectrometry. J. Am. Chem. Soc. 112, 9012-9013 (1990)
4. Konermann, L., Douglas, D.J.: Equilibrium unfolding of proteins monitored by electrospray ionization mass spectrometry: Distinguishing two-state from multi-state transitions. Rapid Commun. Mass Spectrom. 12, 435-442 (1998) (Pubitemid 28181283)
5. Wang, G., Cole, R.B.: Disparity between solution phase equilibria and charge state distributions in positive ion electrospray mass spectrometry. Org. Mass Spectrom. 29, 419-427 (1994)
6. Le Blanc, J.C.Y., Wang, J., Guevremont, R., Siu, K.W.M.: Electrospray mass spectra of protein cations formed in basic solutions. Org. Mass Spectrom. 29, 587-593 (1994)
7. Chillier, X.F.D., Monnier, A., Bill, H., Gulacar, F., Buchs, A., McLuckey, S.A., Van Berkel, G.J.: A mass spectrometry and optical spectroscopy investigation of gas-phase ion formation in electrospray. Rapid Commun. Mass Spectrom. 10, 299 (1996)
8. Iavarone, A.T., Jurchen, J.C., Williams, E.R.: Effects of solvent on the maximum charge state and charge state distribution of protein ions produced by electrospray ionization. J. Am. Soc. Mass Spectrom. 11, 976-985 (2000) (Pubitemid 34546668)
9. Schnier, P.D., Gross, D.S., Williams, E.R.: On the maximum charge state and proton transfer reactivity of peptide and protein ions formed by electrospray ionization. J. Am. Soc. Mass Spectrom. 6, 1086-1097 (1995)
10. Mirza, U., Chait, B.: Effects of anions on the positive-ion electrosprayionization mass-spectra of peptides and proteins. Anal. Chem. 66, 2898 (1994)
11. Page, J., Kelly, R., Tang, K., Smith, R.: Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. J. Am. Soc. Mass Spectrom. 18, 1582-1590 (2007) (Pubitemid 47302317)
12. Williams, E.R.: Proton transfer reactivity of large multiply charged ions. J. Mass Spectrom. 31, 831-842 (1996) (Pubitemid 26258960)
13. Iavarone, A.T., Williams, E.R.: Mechanism of charging and supercharging molecules in electrospray ionization. J. Am. Chem. Soc. 125, 2319-2327 (2003) (Pubitemid 36245725)
14. Reid, G.E., McLuckey, S.A.: Top down protein characterization via tandem mass spectrometry. J. Mass Spectrom. 37, 663-675 (2002) (Pubitemid 34830804)
15. Cui, W., Rohrs, H.W., Gross, M.L.: Top down mass spectrometry: Recent developments, applications, and perspectives. Analyst 136, 3854-3864 (2011)
16. Frey, B.L., Krusemark, C.J., Ledvina, A.R., Coon, J.J., Belshaw, P.J., Smith, L.M.: Ion-ion reactions with fixed-charge modified proteins to produce ions in a single, very high charge state. Int. J. Mass Spectrom. 276, 136 (2008)
17. Iavarone, A.T., Jurchen, J.C., Williams, E.R.: Supercharged protein and peptide ions formed by electrospray ionization. Anal. Chem. 73, 1455-1460 (2001) (Pubitemid 32896200)
18. Iavarone, A.T., Williams, E.R.: Supercharging in electrospray ionization: effects on signal and charge. Int. J. Mass Spectrom. 219, 63-72 (2002)
19. Lomeli, S., Yin, S., Ogorzalek Loo, R., Loo, J.: Increasing charge while preserving noncovalent protein complexes for ESI-MS. J. Am. Soc. Mass Spectrom. 20, 593-596 (2009)
20. Lomeli, S., Peng, I., Yin, S., Ogorzalek Loo, R., Loo, J.: New reagents for increasing ESI multiple charging of proteins and protein complexes. J. Am. Soc. Mass Spectrom. 21, 127-131 (2010)
21. Ganisl, B., Taucher, M., Riml, C., Breuker, K.: Charge as you like! Efficient manipulation of negative ion net charge in electrospray ionization of proteins and nucleic acids. Eur. J. Mass Spectrom. 17, 333 (2011)
22. Zhao, C., Wood, T., Bruckenstein, S.: Shifts in protein charge state distributions with varying redox reagents in nanoelectrospray triple quadrupole mass spectrometry. J. Am. Soc. Mass Spectrom. 16, 409-416 (2005) (Pubitemid 40331919)
23. Scalf, M., Westphall, M.S., Krause, J., Kaufman, S.L., Smith, L.M.: Controlling charge states of large ions. Science 283, 194-197 (1999)
24. Stephenson, J.L., McLuckey, S.A.: Ion/ion reactions in the gas phase: tranfer Proton transfer reactions involving multiply-charged proteins. J. Am. Chem. Soc. 118, 7390-7397 (1996) (Pubitemid 26270627)
25. McLuckey, S.A., Van Berkel, G.J., Glish, G.L.: Reactions of dimethylamine with multiply charged ions of cytochrome c. J. Am. Chem. Soc. 112, 5668-5670 (1990) (Pubitemid 20308614)
26. Loo, R.R.O., Loo, J.A., Udseth, H.R., Fulton, J.L., Smith, R.D.: Protein structural effects in gas phase ion/molecule reactions with diethylamine. Rapid Commun. Mass Spectrom. 6, 159-165 (1992)
27. Hunter, A.P., Severs, J.C., Harris, F.M., Games, D.E.: Proton transfer reactions of mass-selected multiply charged ions. Rapid Commun. Mass Spectrom. 8, 417-422 (1994)
28. Chen, H., Venter, A., Cooks, R.G. Extractive electrospray ionization for direct analysis of undiluted urine, milk, and other complex mixtures without sample preparation. Chem. Commun. 2042-2044 (2006) (Pubitemid 43725994)
29. Yang, S.H., Wijeratne, A.B., Li, L., Edwards, B.L., Schug, K.A.: Manipulation of protein charge states through continuous flowextractive desorption electrospray ionization: A new ambient ionization technique. Anal. Chem. 83, 643-647 (2010)
30. Peng, Y., Zhang, S., Gong, X., Ma, X., Yang, C., Zhang, X.: Controlling charge states of peptides through inductive electrospray ionization mass spectrometry. Anal. Chem. 83, 8863-8866 (2011)
31. Bagal, D., Zhang, H., Schnier, P.D.: Gas-phase proton-Transfer chemistry coupled with TOF mass spectrometry and ion mobility-MS for the facile analysis of poly(ethylene glycols) and PEGylated polypeptide conjugates. Anal. Chem. 80, 2408-2418 (2008) (Pubitemid 351499822)
32. Kharlamova, A., DeMuth, J., McLuckey, S.: Vapor treatment of electrospray droplets: Evidence for the folding of initially denatured proteins on the sub-millisecond time-scale. J. Am. Soc. Mass Spectrom. 23, 88-101 (2012)
33. Kharlamova, A., Prentice, B.M., Huang, T.Y., McLuckey, S.A.: Electrospray droplet exposure to gaseous acids for the manipulation of protein charge state distributions. Anal. Chem. 82, 7422-7429 (2010)
34. Kharlamova, A., McLuckey, S.A.: Negative electrospray droplet exposure to gaseous bases for the manipulation of protein charge state distributions. Anal. Chem. 83, 431-437 (2011)
35. Zhou, S., Prebyl, B.S., Cook, K.D.: Profiling pH changes in the electrospray plume. Anal. Chem. 74, 4885 (2002)
36. Zhou, S., Edwards, A.G., Cook, K.D., Van Berkel, G.J.: Investigation of the electrospray plume by laser-induced fluorescence spectroscopy. Anal. Chem. 71, 769 (1999)
37. Wang, R., Zenobi, R.: Evolution of the solvent polarity in an electrospray plume. J. Am. Soc. Mass Spectrom. 21, 378 (2010)
38. Girod, M., Dagany, X., Antoine, R., Dugourd, P.: Relation between charge state distributions of peptide anions and pH changes in the electrospray plume. A mass spectrometry and optical spectroscopy investigation. Int. J. Mass Spectrom. 308, 41-48 (2011)
39. Girod, M., Dagany, X., Boutou, V., Broyer, M., Antoine, R., Dugourd, P., Mordehai, A., Love, C., Werlich, M., Fjeldsted, J., Stafford, G.: Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: Influence of size and composition of droplets on charge-state distributions of electro-sprayed proteins. Phys. Chem. Chem. Phys. (2012). doi:10.1039/ C1032CP23341J
40. Whitaker, J.E., Hauglan, R.P., Prendergast, F.G.: Spectral and photophysical studies of benzo[c]xanthene dyes: Dual emission pH sensors. Anal. Biochem. 194, 330 Fraunhofer diffraction 344 (1991)
41. Van Berkel, G.J., Kertesz, V.: Using the electrochemistry of the electrospray ion source. Anal. Chem. 79, 5510 (2007)
42. Van Berkel, G.J., Zhou, F., Aronson, J.T.: Changes in bulk solution pH caused by the inherent controlled-current electrolytic process of an electrospray ion source. Int. J. Mass Spectrom. Ion Process. 162, 55-67 (1997) (Pubitemid 127736986)
43. Rohner, T.C., Lion, N., Girault, H.H.: Electrochemical and theoretical aspects of electrospray ionization. Phys. Chem. Chem. Phys. 6, 3056 (2004)
44. Pfeifer, R.J., Hendrick, C.D.: Parametric studies of electrohydrodynamic spraying. AIAA J. 6, 496 (1968)
45. Ikonomou, M.G., Blades, A.T., Kebarle, P.: Investigations of the electrospray interface for liquid chromatography/mass spectrometry. Anal. Chem. 62, 957-967 (1990)
46. Shcherbakov, V.V., Artemkina, Y.M., Ponomareva, T.N., Kirillov, A.D.: Electrical conductivity of the ammonia-water zystem. Russ. J. Inorg. Chem. 54, 277 (2009)
47. King, H.H., Hall, J.L., Ware, G.C.: A study of the density, surface tension, and adsorption in the water-Ammonia system at 20 /C. Am. Chem. Soc. 52, 5130 (1930)
48. Potapov, A.A., Parkhomenko, I.Y.: Dielectric properties of solutions isomorphous with water. Russ. J. Gen. Chem. 75, 34 (2005)
49. Stephenson, R.M.: Mutual solubility of water and pyridine derivatives. J. Chem. Eng. Data 38, 428-431 (1993)
50. Ablard, J.E., McKinney, D.S., Warner, J.C.: The conductance, dissociation constant, and heat of dissociation of triethylamine in water. J. Am. Chem. Soc. 62, 2181-2183 (1940)
51. Campbell, A.N., Lam, S.-Y.: The Dissociation constants and conductivities of mono-, di-, and triethylamine in aqueous solution. Can. J. Chem. 51, 551-555 (1973)
52. Van Berkel, G.J.: The electrolytic nature of electrospray. In: Cole, R. (ed.) Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation and Applications, John Wiley, NY (1997)
53. Mordehai, A.: ESI technology with thermal gradient focusing-theoretical and practical aspects. Proceedings of the 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA, (2009)
54. Nemes, P., Marginean, I., Vertes, A.: Spraying mode effect on Droplet formation and ion chemistry in electrosprays. Anal. Chem. 79, 3105 (2007)
55. Kebarle, P., Tang, L.: From ions in solution to ions in the gas phase-the mechanism of electrospray mass spectrometry. Anal. Chem. 65, 972A (1993)
56. Taflin, D.C., Ward, T.L., Davis, E.J.: Electrified droplet fission and the Rayleigh limit. Langmuir 5, 376 (1989)
57. Lide, D.R.: CRC Handbook of Chemistry and Physics, 70th edn. CRC Press, Boca Raton (1990)
58. Kaltashov, I.A., Eyles, S.J.: Studies of biomolecular conformations and conformational dynamics by mass spectrometry. Mass Spectrom. Rev. 21, 37-71 (2002) (Pubitemid 34950940)
59. Vijay-Kumar, S., Bugg, C.E., Cook, W.J.: Structure of ubiquitin refined at 1.8 Ã resolution. J. Mol. Biol. 194, 531-544 (1987)
60. Konermann, L., Silva, E.A., Sogbein, O.F.: Electrochemically induced pH changes resulting in protein unfolding in the ion source of an electrospray mass spectrometer. Anal. Chem. 73, 4836-4844 (2001) (Pubitemid 32980105)
61. Antonini, E., Brunori, M.: Hemoglobin and myoglobin in their reaction with ligands. North-Holland Publishing Co, Amsterdam (1971)
62. Li, Y.T., Hsieh, Y.L., Henion, J.D., Ganem, B.: Studies on heme binding in myoglobin, hemoglobin, and cytochrome c by ion spray mass spectrometry. J. Am. Soc. Mass Spectrom. 4, 631-637 (1993)
63. Katta, V., Chait, B.T.: Observation of the heme-goblin complex in native myoglobin by electrospray ionization mass spectrometry. J. Am. Chem. Soc. 113, 8534-8535 (1991)
64. Sogbein, O.O., Simmons, D.A., Konermann, L.: Effects of pH on the kinetic reaction mechanism of myoglobin unfolding studied by timeresolved electrospray ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 11, 312-319 (2000) (Pubitemid 34506419)