Feeding genetically modified maize (MON810) to dairy cows: Comparison of gene expression pattern of markers for apoptosis, inflammation and cell cycle

Journal fur Verbraucherschutz und Lebensmittelsicherheit

Volumn 7, Issue 3, 2012, Pages 195-202

  Guertler, Patrick ^a   Brandl, Christina ^a   Meyer, Heinrich H D ^a   Tichopad, Ales ^a,b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b INSTITUTE OF BIOTECHNOLOGY   (Czech Republic)

Author keywords

Apoptosis; Cell cycle; Dairy cow; Gastrointestinal tract; Gene expression; Inflammation; MON810

Indexed keywords

ANIMALIA; BOS; ZEA MAYS;

EID: 84865796326     PISSN: 16615751     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00003-012-0778-8     Document Type: Article

References (44)

1. Adel-Patient K, Guimaraes VD, Paris A, Drumare M-F, Ah-Leung S, Lamourette P, Nevers M-C, Canlet C, Molina J, Bernard H, Créminon C, Wal J-M (2011) Immunological and metabolomic impacts of administration of Cry1Ab protein and MON 810 maize in mouse. PLoS One 6(1): e16346.
2. Ashkenazi A (2008) Directing cancer cells to self-destruct with pro-apoptotic receptor agonists. Nat Rev Drug Discov 7(12): 1001-1012.
3. Bogdanovich S, Krag TO, Barton ER, Morris LD, Whittemore LA, Ahima RS, Khurana TS (2002) Functional improvement of dystrophic muscle by myostatin blockade. Nature 420(6914): 418-421.
4. Bravo A, Gill SS, Soberon M (2007) Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49(4): 423-435.
5. Chowdhury EH, Mikami O, Murata H, Sultana P, Shimada N, Yoshioka M, Guruge KS, Yamamoto S, Miyazaki S, Yamanaka N, Nakajima Y (2004) Fate of maize intrinsic and recombinant genes in calves fed genetically modified maize Bt11. J Food Prot 67(2): 365-370.
6. de Maagd RA, Bravo A, Crickmore N (2001) How Bacillus thuringiensis has evolved specific toxins to colonize the insect world. Trends Genet 17(4): 193-199.
7. de Vendômois JS, Roullier F, Cellier D, Seralini GE (2009) A comparison of the effects of three GM corn varieties on mammalian health. Int J Biol Sci 5(7): 706-726.
8. Ding C, Cantor CR (2004) Quantitative analysis of nucleic acids-the last few years of progress. J Biochem Mol Biol 37(1): 1-10.
9. English L, Slatin SL (1992) Mode of action of delta-endotoxins from Bacillus thuringiensis: a comparison with other bacterial toxins. Insect Biochem Mol Biol 22(1): 1-7.
10. Finamore A, Roselli M, Britti S, Monastra G, Ambra R, Turrini A, Mengheri E (2008) Intestinal and peripheral immune response to MON810 maize ingestion in weaning and old mice. J Agric Food Chem 56(23): 11533-11539.
11. Flachowsky G, Aulrich K (2003) Assessment of novel foods in animal nutrition. Forum Nutr 56: 335-337.
12. Gruber H, Paul V, Guertler P, Spiekers H, Tichopad A, Meyer HH, Muller M (2011) Fate of Cry1Ab protein in agricultural systems under slurry management of cows fed genetically modified maize (Zea mays L.) MON810: a quantitative assessment. J Agric Food Chem 59(13): 7135-7144.
13. Guertler P, Paul V, Albrecht C, Meyer HH (2009) Sensitive and highly specific quantitative real-time PCR and ELISA for recording a potential transfer of novel DNA and Cry1Ab protein from feed into bovine milk. Anal Bioanal Chem 393(6-7): 1629-1638.
14. Guertler P, Paul V, Steinke K, Wiedemann S, Preißinger W, Albrecht C, Spiekers H, Schwarz FJ, Meyer HHD (2010) Long-term feeding of genetically modified corn (MON810)-fate of cry1Ab DNA and recombinant protein during the metabolism of the dairy cow. Livest Sci 131(2-3): 250-259.
15. Höfte H, Whiteley HR (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev 53(2): 242-255.
16. James C (2011) Global status of commercialized biotech/GM crops: 2011. ISAAA Brief No. 43. ISAAA, Ithaca.
17. Karim S, Dean DH (2000) Pesticidal and receptor binding properties of Bacillus thuringiensis Cry1Ab and Cry1Ac delta-endotoxin mutants to Pectinophora gossypiella and Helicoverpa zea. Curr Microbiol 41(6): 430-440.
18. Kliem H, Berisha B, Meyer HH, Schams D (2009) Regulatory changes of apoptotic factors in the bovine corpus luteum after induced luteolysis. Mol Reprod Dev 76(3): 220-230.
19. Kroghsbo S, Madsen C, Poulsen M, Schrøder M, Kvist PH, Taylor M, Gatehouse A, Shu Q, Knudsen I (2008) Immunotoxicological studies of genetically modified rice expressing PHA-E lectin or Bt toxin in Wistar rats. Toxicology 245(1-2): 24-34.
20. Lavrik IN, Golks A, Krammer PH (2005) Caspases: pharmacological manipulation of cell death. J Clin Invest 115(10): 2665-2672.
21. Lee SJ, McPherron AC (1999) Myostatin and the control of skeletal muscle mass. Curr Opin Genet Dev 9(5): 604-607.
22. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4): 402-408.
23. Lutz B, Wiedemann S, Einspanier R, Mayer J, Albrecht C (2005) Degradation of Cry1Ab protein from genetically modified maize in the bovine gastrointestinal tract. J Agric Food Chem 53(5): 1453-1456.
24. Mesnage R, Clair E, Gress S, Then C, Székács A, Séralini GE (2012) Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide. J Appl Toxicol. doi: 10. 1002/jat. 2712.
25. Mestdagh P, van Vlierberghe P, De Weer A, Muth D, Westermann F, Speleman F, Vandesompele J (2009) A novel and universal method for microRNA RT-qPCR data normalization. Genome Biol 10(6): R64.
26. Nguyen HT, Jehle JA (2007) Quantitative analysis of the seasonal and tissue-specific expression of Cry1Ab in transgenic maize MON810. J Plant Dis Prot 114(2): 82-87.
27. Onose J, Imai T, Hasumura M, Ueda M, Ozeki Y, Hirose M (2008) Evaluation of subchronic toxicity of dietary administered Cry1Ab protein from Bacillus thuringiensis var. Kurustaki HD-1 in F344 male rats with chemically induced gastrointestinal impairment. Food Chem Toxicol 46(6): 2184-2189.
28. Paul V, Guertler P, Wiedemann S, Meyer HH (2010) Degradation of Cry1Ab protein from genetically modified maize (MON810) in relation to total dietary feed proteins in dairy cow digestion. Transgenic Res 19(4): 683-689.
29. Pfaffl MW (2006) Relative quantification. In: Dorak T (ed) Real-time PCR. International University Line, La Jolla, CA, USA, pp 63-82.
30. Rani R, Kansal V, Kaushal D, De S (2011) Dietary intervention of cow ghee and soybean oil on expression of cell cycle and apoptosis related genes in normal and carcinogen treated rat mammary gland. Mol Biol Rep 38(5): 3299-3307.
31. Reiter M, Walf VM, Christians A, Pfaffl MW, Meyer HH (2007) Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers. Anal Chim Acta 586(1-2): 73-81.
32. Revelo XS, Waldron MR (2012) In vitro effects of Escherichia coli lipopolysaccharide on the function and gene expression of neutrophils isolated from the blood of dairy cows. J Dairy Res 95(5): 2422-2441.
33. Riedmaier I, Tichopad A, Reiter M, Pfaffl MW, Meyer HH (2009) Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells. Anal Chim Acta 638(1): 106-113.
34. Schnepf E, Crickmore N, van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62(3): 775-806.
35. Shimada N, Miyamoto K, Kanda K, Murata H (2006) Bacillus thuringiensis insecticidal crylab toxin does not affect the membrane integrity of the mammalian intestinal epithelial cells: an in vitro study. In Vitro Cell Dev Biol Anim 42(1): 45-49.
36. Steinke K, Guertler P, Paul V, Wiedemann S, Ettle T, Albrecht C, Meyer HH, Spiekers H, Schwarz FJ (2010) Effects of long-term feeding of genetically modified corn (event MON810) on the performance of lactating dairy cows. J Anim Physiol Anim Nutr (Berl) 94(5): e185-e193.
37. Suzuki T, Higgins PJ, Crawford DR (2000) Control selection for RNA quantitation. Biotechniques 29(2): 332-337.
38. Székács A, Lauber É, Juracsek J, Darvas B (2010a) Cry1Ab toxin production of MON 810 transgenic maize. Environ Toxicol Chem 29(1): 182-190.
39. Székács A, Lauber É, Takács E, Darvas B (2010b) Detection of Cry1Ab toxin in the leaves of MON 810 transgenic maize. Anal Bioanal Chem 396(6): 2203-2211.
40. Vázquez-Padrón RI, Moreno-Fierros L, Neri-Bazán L, de la Riva GA, López-Revilla R (1999) Intragastric and intraperitoneal administration of Cry1Ac protoxin from Bacillus thuringiensis induces systemic and mucosal antibody responses in mice. Life Sci 64(21): 1897-1912.
41. Walsh MC, Buzoianu SG, Gardiner GE, Rea MC, Gelencsé E, Jánosi A, Epstein MM, Ross RP, Lawlor PG (2011) Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs. PLoS One 6(11): e27177.
42. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39(1): 75-85.
43. Xu W, Cao S, He X, Luo Y, Guo X, Yuan Y, Huang K (2009) Safety assessment of Cry1Ab/Ac fusion protein. Food Chem Toxicol 47(7): 1459-1465.
44. Zhang X, Candas M, Griko NB, Rose-Young L, Bulla LA (2005) Cytotoxicity of Bacillus thuringiensis Cry1Ab toxin depends on specific binding of the toxin to the cadherin receptor BT-R1 expressed in insect cells. Cell Death Differ 12(11): 1407-1416.