Stabilization of Candida antarctica lipase B in hydrophilic organic solvent by rational design of hydrogen bond

Biotechnology and Bioprocess Engineering

Volumn 17, Issue 4, 2012, Pages 722-728

  Park, Hyun June ^a   Joo, Jeong Chan ^a   Park, Kyungmoon ^b   Yoo, Young Je ^a  

^a Seoul National University   (South Korea)

^b Hong Ik University   (South Korea)

Author keywords

Candida antarctica lipase B; Hydrogen bond interaction; Organic solvent stability; Protein engineering

Indexed keywords

CANDIDA ANTARCTICA LIPASE B; DESIGN RULES; DIRECTED EVOLUTION; ENZYMATIC REACTION; ENZYME STABILITY; HYDROGEN BOND INTERACTION; HYDROPHILIC SOLVENTS; PROTEIN ENGINEERING; PROTEIN SURFACE; RATIONAL DESIGN; SOLVENT MOLECULES; SOLVENT STABILITY; STABLE MUTANTS; SUBSTRATE SPECIFICITY; WATER MOLECULE; WILD TYPES;

ENZYMES; GENETIC ENGINEERING; HYDROGEN BONDS; HYDROPHILICITY; ISOMERS; REACTION KINETICS; STABILITY;

ORGANIC SOLVENTS;

ALCOHOL; DIMETHYL SULFOXIDE; HYDROGEN; LIPASE B; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT;

ARTICLE; CANDIDA ANTARCTICA; CRYSTAL STRUCTURE; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME STABILITY; FAST PROTEIN LIQUID CHROMATOGRAPHY; HYDROGEN BOND; HYDROPHILICITY; MOLECULAR CLONING; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; WILD TYPE;

CANDIDA ANTARCTICA;

EID: 84865738655     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-012-0092-4     Document Type: Article

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